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42.
Nelson C Soares Maria P Cabral José R Parreira Carmen Gayoso Maria J Barba Germán Bou 《Proteome science》2009,7(1):37-10
Background
Acinetobacter baumannii is a nosocomial pathogen that has been associated with outbreak infections in hospitals. Despite increasing awareness about this bacterium, its proteome remains poorly characterised, however recently the complete genome of A. baumannii reference strain ATCC 17978 has been sequenced. Here, we have used 2-DE and MALDI-TOF/TOF approach to characterise the proteome of this strain. 相似文献43.
Copaifera duckei Oleoresin and Its Main Nonvolatile Terpenes: In Vitro Schistosomicidal Properties 下载免费PDF全文
Carly H. G. Borges Michele G. Cruz Luiza J. Carneiro Jonas J. M. da Silva Jairo K. Bastos Denise C. Tavares Pollyanna F. de Oliveira Vanderlei Rodrigues Rodrigo C. S. Veneziani Renato L. T. Parreira Giovanni F. Caramori Gláucio R. Nagurniak Lizandra G. Magalhães Sérgio R. Ambrósio 《化学与生物多样性》2016,13(10):1348-1356
In this article, the in vitro schistosomicidal effects of three Brazilian Copaifera oleoresins (C. duckei, C. langsdorffii, and C. reticulata) are reported. From these botanical sources, the oleoresin of C. duckei (OCd) demonstrated to be the most promising, displaying LC50 values of 75.8, 50.6, and 47.2 μg/ml at 24, 48, and 72 h of incubation, respectively, against adult worms of Schistosoma mansoni, with a selectivity index of 10.26. Therefore, the major compounds from OCd were isolated, and the diterpene, (?)‐polyalthic acid (PA), showed to be active (LC50 values of 41.7, 36.2, and 33.4 μg/ml, respectively, at 24, 48, and 72 h of incubation). Moreover, OCd and PA affected the production and development of eggs, and OCd modified the functionality of the tegument of S. mansoni. Possible synergistic and/or additive effects of this balsam were also verified when a mixture of the two of its main compounds (PA and ent‐labd‐8(17)‐en‐15,18‐dioic acid) in the specific proportion of 3:1 (w/w) was tested. The obtained results indicate that PA should be considered for further investigations against S. mansoni, such as, synergistic (combination with praziquantel (PZQ)) and in vivo studies. It also shows that diterpenes are an important class of natural compounds for the investigation of agents capable of fighting the parasite responsible for human schistosomiasis. 相似文献
44.
Nascimento Filho V. F. Poblete V. H. Parreira P. S. Matsumoto E. Simabuco S. M. Espinoza E. P. Navarro A. A. 《Biological trace element research》1999,(1):423-430
An X-ray tube with a Mo target and Zr filter, operated at 45 kV/20 mA, was used to excite samples (5 ΜL deposited on a quartz
support) and the total reflection angle condition was obtained with a double reflector module built with two 10-cm-long 7-mm-thick
quartz crystals placed 50 Μm apart. A high-resolution spectrometer based on a Si(Li) detector coupled to a multichannel analyzer
was used for X-ray detection and the spectra were interpreted with the AXIL software.
The system was calibrated with standard chemical solutions containing Cr, Fe, Cu, Zn, and Pb, and Y was used as an internal
standard to correct eventual geometric errors and high-voltage instabilities of the X-ray generator. The limits of detection
were 19, 9, 5, and 4 ng/mL for Cr, Fe, Cu, and Zn, respectively, analyzed through characteristicKk
α
X-rays, and 7 ng/mL for Pb, throughLk
α
X-rays, considering 50 ΜL samples deposited and dried on a quartz support, to be excited/ detected for 1000 s. 相似文献
45.
G G Parreira T Ogawa M R Avarbock L R Fran?a C L Hausler R L Brinster L D Russell 《Tissue & cell》1999,31(3):242-254
Mouse-to-mouse transplants were studied at 10 min, 9 h, 24 h, 1 week, 1 month, 2 months, and 3 months post-transplantation. Data from a previous light microscope study were confirmed and extended using morphometric and ultrastructural techniques. As soon as 10 min after introduction of the germ cells from one mouse into the tubule lumen of a recipient mouse they developed relationships with small Sertoli cell processes. The extent of this surface-to-surface relationship increased in animals sacrificed up to 1 week post-transplantation. Most transplanted germ cells retained the characteristics of the donor germ cells after they had been isolated and pelleted. Nearly all transplanted cells eventually underwent phagocytosis by the recipient Sertoli cells. The presence of small apparent clones of germ cells after 1 week of transplantation indicated that some germ cells may divide and survive for short periods within the epithelium. No discernible qualitative subcellular changes in the host Sertoli cell accompanying the development of transplant spermatogenesis were noted. Macrophages were present in the region of the boundary tissue between myoid cells and appeared to increase in number in the peritubular tissue of transplanted testes. Images suggest that they migrated into the tubule to gain entrance to the lumen and there take on the form of activated macrophages. Some macrophages phagocytose sperm at 2 months and 3 months post-transplantation. A testis weight increase previously demonstrate to occur at 24 h post-introduction of germ cells was found to be due to an increase in the volume of the tubular lumen. The increase of lumen size at 24 h was not related to the volume of the injected material. It is suggested that the presence of injected cells, likely germ cells, in the tubule lumen stimulated increased secretion by the Sertoli cell. 相似文献
46.
Ricardo Alcántara-de la Cruz Douglas Silva Parreira Francisco Andreas Rodríguez Dimaté Lorena Duarte Batista Rafael Coelho Ribeiro José Cola Zanuncio 《Journal of Asia》2021,24(2):64-67
Bioactivity of nine essential oils (EOs) was studied on Anagasta kuehniella eggs in relation to the longevity of females, parasitism and emergence rates and sex ratio in the generations parental, F1 and F2 of Trichogramma galloi in comparison to a trade formulation of Azadirachta indica. There was no F1 and F2 progeny with Zingiber officinale being the most harmful. The greatest reductions in the parasitism rates (57, 43 and 28%) in the parental generation was caused by Allium sativum, Carapa guianensis and A. indica, respectively. In addition, A. sativum reduced the longevity (4.7 days) in the parental generation and emergence (33%) of F1. Tested EOs did not affect the sex ratio in the generations F1 and F2 and emergence in the F2. Allium sativum and Z. officinale were non-selective to T. galloi; while A. indica, C. guianensis and P. nigrum oils may compromise the progeny; therefore, their use must be avoided. Citrus sinensis, Mentha piperita, Origanum vulgare, Syzygium aromaticum and Thymus vulgare were selective to T. galloi, and these EOs are promising for IPM programs. 相似文献
47.
Nayara B. R. Neres Daniel Montagnini Daniele S. Ferreira Renato L. T. Parreira Renato P. Orenha Thais C. Lima Eduardo F. Molina Wilson R. Cunha Márcio L. A. Silva Viviane R. Esperandim 《化学与生物多样性》2021,18(6):e2100052
In this study, the trypomastigotes of a Y strain of Trypanosoma cruzi were inoculated intraperitoneally into male BALB/c mice weighing approximately 25 g each, which were divided into groups for evaluation of the trypanocidal activity. For the treatment of experimental groups, encapsulated and unencapsulated (−)-cubebin, Benznidazole, and two groups as negative controls were used. The encapsulated (−)-cubebin showed a 68.1 % encapsulation efficiency. The parasitemia peak of substances remained around the 9th day after the observed reduction in the number of circulating trypomastigotes. The encapsulated (−)-cubebin and (−)-cubebin unloaded showed a decrease of 61.3 % and 58.5 % in the number of parasites as compared to the negative control, respectively. Moreover, animals treated with encapsulated (−)-cubebin had a higher survival time as compared to the other groups. In conclusion, the results obtained were more promising for encapsulated (−)-cubebin as compared to unloaded particles. 相似文献
48.
Carlos DeOcesano-Pereira Murilo S. Amaral Kleber S. Parreira Ana C. Ayupe Jacqueline F. Jacysyn Gustavo P. Amarante-Mendes Eduardo M. Reis Sergio Verjovski-Almeida 《Nucleic acids research》2014,42(13):8343-8355
BCL-X mRNA alternative splicing generates pro-apoptotic BCL-XS or anti-apoptotic BCL-XL gene products and the mechanism that regulates splice shifting is incompletely understood. We identified and characterized a long non-coding RNA (lncRNA) named INXS, transcribed from the opposite genomic strand of BCL-X, that was 5- to 9-fold less abundant in tumor cell lines from kidney, liver, breast and prostate and in kidney tumor tissues compared with non-tumors. INXS is an unspliced 1903 nt-long RNA, is transcribed by RNA polymerase II, 5′-capped, nuclear enriched and binds Sam68 splicing-modulator. Three apoptosis-inducing agents increased INXS lncRNA endogenous expression in the 786-O kidney tumor cell line, increased BCL-XS/BCL-XL mRNA ratio and activated caspases 3, 7 and 9. These effects were abrogated in the presence of INXS knockdown. Similarly, ectopic INXS overexpression caused a shift in splicing toward BCL-XS and activation of caspases, thus leading to apoptosis. BCL-XS protein accumulation was detected upon INXS overexpression. In a mouse xenograft model, intra-tumor injections of an INXS-expressing plasmid caused a marked reduction in tumor weight, and an increase in BCL-XS isoform, as determined in the excised tumors. We revealed an endogenous lncRNA that induces apoptosis, suggesting that INXS is a possible target to be explored in cancer therapies. 相似文献
49.
Satish Kumar Rajasekhara Reddy Ravuri Padmaja Koneru BP Urade BN Sarkar A Chandrasekar VR Rao 《BMC evolutionary biology》2009,9(1):173-5
Background
An early dispersal of biologically and behaviorally modern humans from their African origins to Australia, by at least 45 thousand years via southern Asia has been suggested by studies based on morphology, archaeology and genetics. However, mtDNA lineages sampled so far from south Asia, eastern Asia and Australasia show non-overlapping distributions of haplogroups within pan Eurasian M and N macrohaplogroups. Likewise, support from the archaeology is still ambiguous. 相似文献50.
Cabral MP Soares NC Aranda J Parreira JR Rumbo C Poza M Valle J Calamia V Lasa I Bou G 《Journal of proteome research》2011,10(8):3399-3417
Biofilm formation is one of the main causes for the persistence of Acinetobacter baumannii, a pathogen associated with severe infections and outbreaks in hospitals. Here, we performed comparative proteomic analyses (2D-DIGE and MALDI-TOF/TOF and iTRAQ/SCX-LC-MS/MS) of cells at three different conditions: exponential, late stationary phase, and biofilms. These results were compared with alterations in the proteome resulting from exposure to a biofilm inhibitory compound (salicylate). Using this multiple-approach strategy, proteomic patterns showed a unique lifestyle for A. baumannii biofilms and novel associated proteins. Several cell surface proteins (such as CarO, OmpA, OprD-like, DcaP-like, PstS, LysM, and Omp33), as well as those involved in histidine metabolism (like Urocanase), were found to be implicated in biofilm formation, this being confirmed by gene disruption. Although l-His uptake triggered biofilms efficiently in wild-type A. baumannii, no effect was observed in Urocanase and OmpA mutants, while a slight increase was observed in a CarO deficient strain. We conclude that Urocanase plays a crucial role in histidine metabolism leading to biofilm formation and that OmpA and CarO can act as channels for L-His uptake. Finally, we propose a model in which novel proteins are suggested for the first time as targets for preventing the formation of A. baumannii biofilms. 相似文献