全文获取类型
收费全文 | 5752篇 |
免费 | 561篇 |
国内免费 | 4篇 |
出版年
2021年 | 66篇 |
2020年 | 38篇 |
2019年 | 34篇 |
2018年 | 57篇 |
2017年 | 47篇 |
2016年 | 96篇 |
2015年 | 172篇 |
2014年 | 156篇 |
2013年 | 189篇 |
2012年 | 272篇 |
2011年 | 306篇 |
2010年 | 173篇 |
2009年 | 144篇 |
2008年 | 243篇 |
2007年 | 265篇 |
2006年 | 207篇 |
2005年 | 244篇 |
2004年 | 230篇 |
2003年 | 248篇 |
2002年 | 194篇 |
2001年 | 184篇 |
2000年 | 153篇 |
1999年 | 147篇 |
1998年 | 70篇 |
1997年 | 63篇 |
1996年 | 58篇 |
1995年 | 48篇 |
1994年 | 45篇 |
1993年 | 37篇 |
1992年 | 93篇 |
1991年 | 98篇 |
1990年 | 73篇 |
1989年 | 84篇 |
1988年 | 54篇 |
1987年 | 74篇 |
1986年 | 49篇 |
1985年 | 76篇 |
1984年 | 77篇 |
1983年 | 56篇 |
1982年 | 59篇 |
1981年 | 68篇 |
1980年 | 67篇 |
1979年 | 74篇 |
1978年 | 69篇 |
1977年 | 55篇 |
1976年 | 50篇 |
1975年 | 60篇 |
1974年 | 52篇 |
1973年 | 42篇 |
1972年 | 34篇 |
排序方式: 共有6317条查询结果,搜索用时 15 毫秒
991.
992.
993.
Presence of Acetyl Coenzyme A (CoA) Carboxylase and Propionyl-CoA Carboxylase in Autotrophic Crenarchaeota and Indication for Operation of a 3-Hydroxypropionate Cycle in Autotrophic Carbon Fixation 总被引:2,自引:1,他引:1 下载免费PDF全文
Castor Menendez Zsuzsa Bauer Harald Huber Nasser Gadon Karl-Otto Stetter Georg Fuchs 《Journal of bacteriology》1999,181(4):1088-1098
The pathway of autotrophic CO2 fixation was studied in the phototrophic bacterium Chloroflexus aurantiacus and in the aerobic thermoacidophilic archaeon Metallosphaera sedula. In both organisms, none of the key enzymes of the reductive pentose phosphate cycle, the reductive citric acid cycle, and the reductive acetyl coenzyme A (acetyl-CoA) pathway were detectable. However, cells contained the biotin-dependent acetyl-CoA carboxylase and propionyl-CoA carboxylase as well as phosphoenolpyruvate carboxylase. The specific enzyme activities of the carboxylases were high enough to explain the autotrophic growth rate via the 3-hydroxypropionate cycle. Extracts catalyzed the CO2-, MgATP-, and NADPH-dependent conversion of acetyl-CoA to 3-hydroxypropionate via malonyl-CoA and the conversion of this intermediate to succinate via propionyl-CoA. The labelled intermediates were detected in vitro with either 14CO2 or [14C]acetyl-CoA as precursor. These reactions are part of the 3-hydroxypropionate cycle, the autotrophic pathway proposed for C. aurantiacus. The investigation was extended to the autotrophic archaea Sulfolobus metallicus and Acidianus infernus, which showed acetyl-CoA and propionyl-CoA carboxylase activities in extracts of autotrophically grown cells. Acetyl-CoA carboxylase activity is unexpected in archaea since they do not contain fatty acids in their membranes. These aerobic archaea, as well as C. aurantiacus, were screened for biotin-containing proteins by the avidin-peroxidase test. They contained large amounts of a small biotin-carrying protein, which is most likely part of the acetyl-CoA and propionyl-CoA carboxylases. Other archaea reported to use one of the other known autotrophic pathways lacked such small biotin-containing proteins. These findings suggest that the aerobic autotrophic archaea M. sedula, S. metallicus, and A. infernus use a yet-to-be-defined 3-hydroxypropionate cycle for their autotrophic growth. Acetyl-CoA carboxylase and propionyl-CoA carboxylase are proposed to be the main CO2 fixation enzymes, and phosphoenolpyruvate carboxylase may have an anaplerotic function. The results also provide further support for the occurrence of the 3-hydroxypropionate cycle in C. aurantiacus. 相似文献
994.
Cloning and Characterization of a Gene (mspA) Encoding the Major Sheath Protein of Treponema maltophilum ATCC 51939T 下载免费PDF全文
Klaus Heuner Bong-Kyu Choi Rüdiger Schade Annette Moter Albrecht Otto Ulf B. Gbel 《Journal of bacteriology》1999,181(3):1025-1029
The major sheath protein-encoding gene (mspA) of the oral spirochete Treponema maltophilum ATCC 51939T was cloned by screening a genomic library with an anti-outer membrane fraction antibody. The mspA gene encodes a precursor protein of 575 amino acids with a predicted molecular mass of 62.3 kDa, including a signal peptide of 19 amino acids. The native MspA formed a heat-modifiable, detergent- and trypsin-stable complex which is associated with the outer membrane. Hybridization with an mspA-specific probe showed no cross-reactivity with the msp gene from Treponema denticola. 相似文献
995.
I M Kooter N Moguilevsky A Bollen L A van der Veen C Otto H L Dekker R Wever 《The Journal of biological chemistry》1999,274(38):26794-26802
The heme group of myeloperoxidase is covalently linked via two ester bonds to the protein and a unique sulfonium ion linkage involving Met(243). Mutation of Met(243) into Thr, Gln, and Val, which are the corresponding residues of eosinophil peroxidase, lactoperoxidase, and thyroid peroxidase, respectively, and into Cys was performed. The Soret band in the optical absorbance spectrum in the oxidized mutants is now found at approximately 411 nm. Both the pyridine hemochrome spectra and resonance Raman spectra of the mutants are affected by the mutation. In the Met(243) mutants the affinity for chloride has decreased 100-fold. All mutants have lost their chlorination activity, except for the M243T mutant, which still has 15% activity left. By Fourier transform infared difference spectroscopy it was possible to specifically detect the (13)CD(3)-labeled methionyl sulfonium ion linkage. We conclude that the sulfonium ion linkage serves two roles. First, it serves as an electron-withdrawing substituent via its positive charge, and, second, together with its neighboring residue Glu(242), it appears to be responsible for the lower symmetry of the heme group and distortion from the planar conformation normally seen in heme-containing proteins. 相似文献
996.
Mutation Analysis of Core Binding Factor A1 in Patients with Cleidocranial Dysplasia 总被引:5,自引:0,他引:5 下载免费PDF全文
997.
998.
Das Wasserdefizit von Gefässpflanzen in verschiedenen Klimazonen 总被引:2,自引:0,他引:2
Otto Stocker 《Planta》1929,7(2-3):382-387
Ohne ZusammenfassungMit 1 Textabbildung. 相似文献
999.
The crystal structure of the Physarum polycephalum actin-fragmin kinase: an atypical protein kinase with a specialized substrate-binding domain. 下载免费PDF全文
S Steinbacher P Hof L Eichinger M Schleicher J Gettemans J Vandekerckhove R Huber J Benz 《The EMBO journal》1999,18(11):2923-2929
Coordinated temporal and spatial regulation of the actin cytoskeleton is essential for diverse cellular processes such as cell division, cell motility and the formation and maintenance of specialized structures in differentiated cells. In plasmodia of Physarum polycephalum, the F-actin capping activity of the actin-fragmin complex is regulated by the phosphorylation of actin. This is mediated by a novel type of protein kinase with no sequence homology to eukaryotic-type protein kinases. Here we present the crystal structure of the catalytic domain of the first cloned actin kinase in complex with AMP at 2.9 A resolution. The three-dimensional fold reveals a catalytic module of approximately 160 residues, in common with the eukaryotic protein kinase superfamily, which harbours the nucleotide binding site and the catalytic apparatus in an inter-lobe cleft. Several kinases that share this catalytic module differ in the overall architecture of their substrate recognition domain. The actin-fragmin kinase has acquired a unique flat substrate recognition domain which is supposed to confer stringent substrate specificity. 相似文献
1000.
Crystal structure of MunI restriction endonuclease in complex with cognate DNA at 1.7 A resolution. 下载免费PDF全文
The MunI restriction enzyme recognizes the palindromic hexanucleotide sequence C/AATTG (the '/' indicates the cleavage site). The crystal structure of its active site mutant D83A bound to cognate DNA has been determined at 1.7 A resolution. Base-specific contacts between MunI and DNA occur exclusively in the major groove. While DNA-binding sites of most other restriction enzymes are comprised of discontinuous sequence segments, MunI combines all residues involved in the base-specific contacts within one short stretch (residues R115-R121) located at the N-terminal region of the 3(10)4 helix. The outer CG base pair of the recognition sequence is recognized solely by R115 through hydrogen bonds made by backbone and side chain atoms to both bases. The mechanism of recognition of the central AATT nucleotides by MunI is similar to that of EcoRI, which recognizes the G/AATTC sequence. The local conformation of AATT deviates from the typical B-DNA form and is remarkably similar to EcoRI-DNA. It appears to be essential for specific hydrogen bonding and recognition by MunI and EcoRI. 相似文献