全文获取类型
收费全文 | 418篇 |
免费 | 31篇 |
出版年
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 11篇 |
2020年 | 3篇 |
2019年 | 8篇 |
2018年 | 9篇 |
2017年 | 7篇 |
2016年 | 20篇 |
2015年 | 21篇 |
2014年 | 20篇 |
2013年 | 52篇 |
2012年 | 37篇 |
2011年 | 33篇 |
2010年 | 20篇 |
2009年 | 20篇 |
2008年 | 22篇 |
2007年 | 31篇 |
2006年 | 20篇 |
2005年 | 19篇 |
2004年 | 17篇 |
2003年 | 18篇 |
2002年 | 15篇 |
2001年 | 1篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1997年 | 3篇 |
1996年 | 3篇 |
1995年 | 5篇 |
1994年 | 5篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1962年 | 1篇 |
1957年 | 1篇 |
排序方式: 共有449条查询结果,搜索用时 15 毫秒
441.
442.
443.
444.
Helicobacter pylori is a major chronic health problem, infecting more than half of the population worldwide. H. pylori infection is linked with various clinical complications ranging from gastritis to gastric cancer. The resolution of gastritis and peptic ulcer appears to be linked with the eradication of H. pylori. However, resistance to antibiotics and eradication failure rates are reaching alarmingly high levels. This calls for urgent action in finding alternate methods for H. pylori eradication. Here, we discuss the recently identified mechanism of H. pylori known as cholesterol glucosylation, mediated by the enzyme cholesterol-α-glucosyltransferase, encoded by the gene cgt. Cholesterol glucosylation serves several functions that include promoting immune evasion, enhancing antibiotic resistance, maintaining the native helical morphology, and supporting functions of prominent virulence factors such as CagA and VacA. Consequently, strategies aiming at inhibition of the cholesterol glucosylation process have the potential to attenuate the potency of H. pylori infection and abrogate H. pylori immune evasion capabilities. Knockout of H. pylori cgt results in unsuccessful colonization and elimination by the host immune responses. Moreover, blocking cholesterol glucosylation can reverse antibiotic susceptibility in H. pylori. In this work, we review the main roles of cholesterol glucosylation in H. pylori and evaluate whether this mechanism can be targeted for the development of alternate methods for eradication of H. pylori infection. 相似文献
445.
446.
Jennifer D. Whitesell Adam Liska Ludovico Coletta Karla E. Hirokawa Phillip Bohn Ali Williford Peter A. Groblewski Nile Graddis Leonard Kuan Joseph E. Knox Anh Ho Wayne Wakeman Philip R. Nicovich Thuc Nghi Nguyen Cindy T.J. van Velthoven Emma Garren Olivia Fong Maitham Naeemi Julie A. Harris 《Neuron》2021,109(3):545-559.e8
447.
Ermanno Florio Simona Keller Lorena Coretti Ornella Affinito Giovanni Scala Francesco Errico 《Epigenetics》2017,12(1):41-54
We performed ultra-deep methylation analysis at single molecule level of the promoter region of developmentally regulated D-Aspartate oxidase (Ddo), as a model gene, during brain development and embryonic stem cell neural differentiation. Single molecule methylation analysis enabled us to establish the effective epiallele composition within mixed or pure brain cell populations. In this framework, an epiallele is defined as a specific combination of methylated CpG within Ddo locus and can represent the epigenetic haplotype revealing a cell-to-cell methylation heterogeneity. Using this approach, we found a high degree of polymorphism of methylated alleles (epipolymorphism) evolving in a remarkably conserved fashion during brain development. The different sets of epialleles mark stage, brain areas, and cell type and unravel the possible role of specific CpGs in favoring or inhibiting local methylation. Undifferentiated embryonic stem cells showed non-organized distribution of epialleles that apparently originated by stochastic methylation events on individual CpGs. Upon neural differentiation, despite detecting no changes in average methylation, we observed that the epiallele distribution was profoundly different, gradually shifting toward organized patterns specific to the glial or neuronal cell types. Our findings provide a deep view of gene methylation heterogeneity in brain cell populations promising to furnish innovative ways to unravel mechanisms underlying methylation patterns generation and alteration in brain diseases. 相似文献
448.
449.
Alberto Conforti Ludovico Medolago-Albani Luciano Alessio 《Virchows Archiv. B, Cell pathology including molecular pathology》1976,22(1):143-149
The ultrastructural changes of human leukemic cell nuclei have been investigated.
Particular attention is paid to the alteration of the nuclear envelope and its constituents, i.e., the pores and the Zonula
Nucleum Limitans which appear constantly involved in these pathologic processes. An alteration of the relationships between
the components of the nuclear envelope and the chromatin itself may be responsible for the appearance of the most nuclear
changes. 相似文献