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21.
Plants of the fern Matteuccia struthiopteris from northern and southern populations in Norway were studied in a phytotron. Relative growth rate (RGR), growth period, and sporophyll production were measured under different photoperiod (12–24 h) day-length and temperature (9–21 °C) treatments. For the southern plants, there were no significant differences between the different light treatments, but for the northern plants there was a significant (p<0.01) linear increase in the mean maximum RGR with increasing day-length. Small, but statistically significant (p<0.01) differences were found between northern and southern plants when the mean maximum RGR-values were compared. When plants from different origin were exposed to different treatment, there were major differences between the populations in the production of sporophylls (p<0.0001). Southern plants produced in average three times more sporophylls than the northern plants, and they had also higher proportions of fertile plants. Diurnal alternating temperature treatments gave no significant (p>0.05) effect on the mean maximum RGR compared with constant temperatures, but they gave significantly higher production of sporophylls. In general, the northern plants had a higher temperature threshold (approximately 12 °C) for sporophyll production than the southern plants (approximately 9 °C). Plants exposed to 24 h with natural light were generally more often fertile than plants exposed to a shorter photoperiod. The mean maximum RGR-values and time needed to develop the fronds at the 9 °C treatment were fairly equal to those found under natural conditions close to the altitudinal distribution limit of M.struthiopteris in W Norway. In general, the investigation showed that the applied temperature and light treatments affected sporophyll production more than vegetative growth. 相似文献
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Tolerance to grazing is a plant trait that can be adaptive in systems where plants are subjected to a diversity of herbivore attack types. To test the tolerance ability of the clonal sedge Carex bigelowii, which is food plant to several herbivores in alpine and arctic areas, and the potential fitness costs of this tolerance, replicated units of genets were subjected to three levels of damage throughout three consecutive seasons. The three levels of treatment were no damage, light damage and heavy damage, and the damage was conducted by tearing off all plant material at 3 and 0 cm above-ground respectively. The genets had no tolerance under damage in terms of sexual reproduction. In terms of clonal reproduction the genets showed tolerance under light damage but not under heavy damage. However, no fitness cost was found for this tolerance ability, i.e. genets had higher reproduction and growth under no damage. The average ramet weight had a similar decrease under both a low and high damage treatment. Changed partitioning of biomass between plant parts and reduced concentration of total non-structural carbohydrates (TNC) in storage organs are possible mechanisms for the ability to uphold clonal reproduction in response to damage. There were no significant indications that tolerance ability or its fitness cost differed between genets. Our results suggest that when subjected to heavy damage genets will only reproduce vegetatively. Consequently, it seems C. bigelowii has evolved to allocate resources to the survival of an already successful genet rather than to a potential new genet of unknown success. 相似文献
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Coecke S Ahr H Blaauboer BJ Bremer S Casati S Castell J Combes R Corvi R Crespi CL Cunningham ML Elaut G Eletti B Freidig A Gennari A Ghersi-Egea JF Guillouzo A Hartung T Hoet P Ingelman-Sundberg M Munn S Janssens W Ladstetter B Leahy D Long A Meneguz A Monshouwer M Morath S Nagelkerke F Pelkonen O Ponti J Prieto P Richert L Sabbioni E Schaack B Steiling W Testai E Vericat JA Worth A 《Alternatives to laboratory animals : ATLA》2006,34(1):49-84
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Sevior DK Pelkonen O Ahokas JT 《The international journal of biochemistry & cell biology》2012,44(2):257-261
Liver is the most important organ involved in biotransformation of xenobiotics. Within the main organisational unit, the hepatocyte, is an assembly of enzymes commonly classified as phase I and phase II enzymes. The phase I enzymes principally cytochrome P450 catalyse both oxidative and reductive reactions of a bewildering number of xenobiotics. Many of the products of phase I enzymes become substrates for the phase II enzymes, which catalyse conjugation reactions making use of endogenous cofactors. As xenobiotic metabolising enzymes are responsible for the toxicity of many chemicals and drugs, testing the role of the biotransformation enzymes and the transporters within the hepatocyte is critical. New methodologies may be able to provide information to allow for better in vitro to in vivo extrapolation of data. 相似文献
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In searching for possible determinants of the ability of compounds to induce microsomal drug metabolizing enzymes we studied relationships between the relative inducing activity of eleven barbiturates and their lipid solubility and pharmacokinetic behaviour. Allyl derivatives of barbituric acid were poor inducers regardless of their lipid solubility or half-life. The ability of other barbiturate derivatives to induce drug metabolism was directly related to biological half-life or time/concentration area value and inversely correlated to lipid solubility. The results suggest that the ability of a given compound to induce drug metabolism is not only related to lipid solubility and pharmacokinetic characteristics but also to the nature of chemical groups present in the molecule. 相似文献
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Cold-Induced Freezing Tolerance in Arabidopsis 总被引:34,自引:0,他引:34
Changes in the physiology of plant leaves are correlated with enhanced freezing tolerance and include accumulation of compatible solutes, changes in membrane composition and behavior, and altered gene expression. Some of these changes are required for enhanced freezing tolerance, whereas others are merely consequences of low temperature. In this study we demonstrated that a combination of cold and light is required for enhanced freezing tolerance in Arabidopsis leaves, and this combination is associated with the accumulation of soluble sugars and proline. Sugar accumulation was evident within 2 h after a shift to low temperature, which preceded measured changes in freezing tolerance. In contrast, significant freezing tolerance was attained before the accumulation of proline or major changes in the percentage of dry weight were detected. Many mRNAs also rapidly accumulated in response to low temperature. All of the cold-induced mRNAs that we examined accumulated at low temperature even in the absence of light, when there was no enhancement of freezing tolerance. Thus, the accumulation of these mRNAs is insufficient for cold-induced freezing tolerance. 相似文献