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排序方式: 共有137条查询结果,搜索用时 691 毫秒
81.
Antonio Cabrera Mary Guttieri Nathan Smith Edward Souza Anne Sturbaum Duc Hua Carl Griffey Marla Barnett Paul Murphy Herb Ohm Jim Uphaus Mark Sorrells Elliot Heffner Gina Brown-Guedira David Van Sanford Clay Sneller 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2015,128(11):2227-2242
82.
Geranylgeranyl pyrophosphate is crucial for neuronal survival but has no special role in Purkinje cell degeneration in Niemann Pick type C1 disease 下载免费PDF全文
Nils Marschalek Frank Albert Sarah Afshordel Volker Meske Gunter P. Eckert Thomas G. Ohm 《Journal of neurochemistry》2015,133(1):153-161
Niemann Pick type C (NPC1) is a rare fatal hereditary cholesterol storage disease associated with a massive Purkinje cells loss. The mechanisms leading to neurodegeneration are still poorly understood. Different laboratories pointed to hypersensitivity to cytotoxic effects of statins (HMG‐CoA reductase inhibitors) in NPC1 and suggested an underlying lack of geranylgeranyl pyrophosphate (GGPP). GGPP is a non‐sterol isoprenoid essential for cell survival and differentiation. We measured GGPP levels in cerebella of a NPC1 mouse model and of wild‐type littermates and found a physiological increase of GGPP levels between post‐natal days 21 and 49 in wild‐type mice but not in NPC mice. This further supports the hypothesis that Purkinje cell loss may be due to an extremely low level of GGPP. The progressive Purkinje cell loss in NPC starts between p21 and p49. To test the hypothesis, we used long‐term organotypic slice cultures of NPC1 mice that display the natural history of NPC1 disease in vitro and tested if chronic administration of GGPP might prevent Purkinje cell loss. We did not see a beneficial effect. This suggests, in contrast to the expectations, that the relative lack of GGPP may not significantly contribute to mechanisms of Purkinje cell loss in NPC1.
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86.
Mapping QTL for popping expansion volume in popcorn with simple sequence repeat markers 总被引:9,自引:0,他引:9
Lu HJ Bernardo R Ohm HW 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,106(3):423-427
Popping expansion volume is the most important quality trait in popcorn ( Zea mays L.), but its genetics is not well understood. The objectives of this study were to map quantitative trait loci (QTLs) responsible for popping expansion volume in a popcorn x dent corn cross, and to compare the predicted efficiencies of phenotypic selection, marker-based selection, and marker-assisted selection for popping expansion volume. Of 259 simple sequence repeat (SSR) primer pairs screened, 83 pairs were polymorphic between the H123 (dent corn) and AG19 (popcorn) parental inbreds. Popping test data were obtained for 160 S(1) families developed from the [AG19(H123 x AG19)] BC(1) population. The heritability ( h(2)) for popping expansion volume on an S(1) family mean basis was 0.73. The presence of the gametophyte factor Ga1(s) in popcorn complicates the analysis of popcorn x dent corn crosses. But, from a practical perspective, the linkage between a favorable QTL allele and Ga1(s) in popcorn will lead to selection for the favorable QTL allele. Four QTLs, on chromosomes 1S, 3S, 5S and 5L, jointly explained 45% of the phenotypic variation. Marker-based selection for popping expansion volume would require less time and work than phenotypic selection. But due to the high h(2) of popping expansion volume, marker-based selection was predicted to be only 92% as efficient as phenotypic selection. Marker-assisted selection, which comprises index selection on phenotypic and marker scores, was predicted to be 106% as efficient as phenotypic selection. Overall, our results suggest that phenotypic selection will remain the preferred method for selection in popcorn x dent corn crosses. 相似文献
87.
Peter?Van LeeuwenEmail author Daniel?Geue Silke?Lange Dirk?Cysarz Henrik?Bettermann Dietrich?HW?Gr?nemeyer 《BMC physiology》2003,3(1):2
Background
The prenatal condition offers a unique possibility of examining physiological interaction between individuals. Goal of this work was to look for evidence of coordination between fetal and maternal cardiac systems.Methods
177 magnetocardiograms were recorded in 62 pregnancies (16th–42nd week of gestation). Fetal and maternal RR interval time series were constructed and the phases, i.e. the timing of the R peaks of one time series in relation to each RR interval of the other were determined. The distributions of these phases were examined and synchrograms were constructed for real and surrogate pairs of fetal and maternal data sets. Synchronization epochs were determined for defined n:m coupling ratios.Results
Differences between real and surrogate data could not be found with respect to number of synchronization epochs found (712 vs. 741), gestational age, subject, recording or n:m combination. There was however a preference for the occurrence of synchronization epochs in specific phases in real data not apparent in the surrogate for some n:m combinations.Conclusion
The results suggest that occasional coupling between fetal and maternal cardiac systems does occur.88.
Dweikat I Zhang W Ohm H 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,105(5):766-770
Hessian fly is one of the world's most destructive insect pests of wheat Triticum aestivum L. We have used the combination of near-isogenic lines (NIL) and random amplified polymorphic DNA (RAPD) analysis to screen up to 2,000 primers to identify DNA markers that are linked to gene H6 that confers resistance to biotype B of the insect. This screen produced six primers that show polymorphic fragments associated with resistance by H6. We have screened 440 F2 individuals from a cross of the susceptible cultivar Newton and a NIL that contains H6 to verify the linkage between these markers and the resistance gene. A high-resolution genetic map was constructed based on recombination frequency. Two of the markers were tightly linked to the gene with no recombination observed, three were within 2.0 cM, and one was 11 cM from the gene. Three of the six markers were successfully converted to sequence tagged site (STS) markers. Both RAPD and STS primers were used to screen for the presence or absence of the resistance gene in wheat varieties. The identification of markers and construction of the genetic high resolution map provide the first steps toward localization of this resistance gene. 相似文献
89.
Francki MG Berzonsky WA Ohm HW Anderson JM 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(2-3):184-191
Cereal centromeres consist of a complex organization of repetitive DNA sequences. Several repetitive DNA sequences are common
amongst members of the Triticeae family, and others are unique to particular species. The organization of these repetitive elements and the abundance of other
types of DNA sequences in cereal centromeres are largely unknown. In this study, we have used wheat-rye translocation lines
to physically map 1BL.1RS centromeric breakpoints and molecular probes to obtain further information on the nature of other
types of centromeric DNA sequences. Our results, using the rye-specific centromeric sequence, pAWRC.1, indicate that 1BL.1RS
contains a small portion of the centromere from 1R of rye. Further studies used molecular markers to identify centromeric
segments on wheat group-1 chromosomes. Selected RFLP markers, clustered around the centromere of wheat homoeologous group-1S
chromosomes, were chosen as probes during Southern hybridization. One marker, PSR161, identified a small 1BS segment in all
1BL.1RS lines. This segment maps proximal to pAWRC.1 in 1BL.1RS and on the centromere of 1B. Sequence analysis of PSR161 showed
high homology to HSP70 genes and Northern hybridization showed that this gene is constitutively expressed in leaf tissue and induced by heat shock
and light stimuli. The significance of this work with respect to centromere organization and the possible significance of
this HSP70 gene homologue are discussed.
Received: 12 March 2001 / Accepted: 14 June 2001 相似文献
90.
In 1998–2000 a monitoring of the spectrum of Fusarium species on winter wheat was carried out in the Rhineland. The epidemic spread ofFusarium spp. on wheat plants during growing season was investigated as well as the grain contamination after harvest.F avenaceum was the Fusarium species isolated most frequently followed byF culmorum, F poae andF graminearum. Microdochium nivale occurred considerably only in 1998. Both, susceptibility and plant height of the cultivars were correlated to the incidence of Fusarium species /M nivale on harvested kernels; interactions with cropping intensities were detected. The incidence ofF poae seemed to be independent of the cultivar-specific Fusarium susceptibility. Despite the lack of disease symptoms, between growth stages 45–85 mycelium ofFusarium spp. was detectable in the leaves as well as conidia on the leaf surfaces. 相似文献