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DAVID G. GARBUZ OLGA G. ZATSEPINA ANDREY A. PRZHIBORO IRINA YUSHENOVA IRINA V. GUZHOVA MICHAEL B. EVGEN’EV 《Molecular ecology》2008,17(21):4763-4777
A population of Stratiomys japonica, a species belonging to the family Stratiomyidae (Diptera), common name ‘soldier flies’, occurs in a hot volcanic spring, which is apparently among the most inhospitable environments for animals because of chemical and thermal conditions. Larvae of this species, which naturally often experience temperatures more than 40 °C, have constitutively high concentrations of the normally inducible heat-shock protein Hsp70, but very low level of corresponding mRNA. Larvae of three other species of the same family, Stratiomys singularior, Nemotelus bipunctatus and Oxycera pardalina, are confined to different type semi-aquatic habitats with contrasting thermal regime. However, all of them shared the same pattern of Hsp70 expression. Interestingly, heat-shock treatment of S. japonica larvae activates heat-shock factor and significantly induces Hsp70 synthesis, whereas larvae of O. pardalina, a species from constant cold environment, produce significantly less Hsp70 in response to heat shock. Adults of the four species also exhibit lower, but detectable levels of Hsp70 without heat shock. Larvae of all species studied have very high tolerance to temperature stress in comparison with other Diptera species investigated, probably representing an inherent adaptive feature of all Stratiomyidae enabling successful colonization of highly variable and extreme habitats. 相似文献
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Detailed knowledge of the composition of protein complexes is crucial for the understanding of their structure and function; however, appropriate techniques for compositional analyses of complexes largely rely on elaborate tagging, immunoprecipitation, cross-linking and purification strategies. The proteasome is a prototypical protein complex and therefore an excellent model to assess new methods for protein complex characterisation. Here we evaluated the applicability of Blue Native (BN) PAGE in combination with label-free protein quantification and protein correlation profiling (PCP) for the investigation of proteasome complexes directly from biological samples. Using the purified human 20S proteasome we showed that the approach can accurately detect members of a complex by clustering their gel migration profiles. We applied the approach to address proteasome composition in the schizont stage of the malaria parasite Plasmodium falciparum. The analysis, performed in the background of the whole protein extract, revealed that all subunits comigrated and formed a tight cluster with a single maximum, demonstrating presence of a single form of the 20S proteasome. This study shows that BN PAGE in combination with label-free quantification and PCP is applicable to the analysis of multiprotein complexes directly from complex protein mixtures. 相似文献
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Benjamin J. Taylor Erik V. Nordheim Robert L. Jeanne 《Ethology : formerly Zeitschrift fur Tierpsychologie》2012,118(6):594-605
In social insects, selection takes place primarily at the level of the colony. Therefore, unlike solitary insects, social species are expected to forage at rates that maximize colony fitness rather than individual fitness. Workers can increase the net benefit of foraging by responding to increased resource availability, by responding more strongly to higher‐quality resources, and by decreasing the uncertainty with which nestmates find resources. Unlike many ants and social bees, no social wasp is known to utilize a nest‐based recruitment signal to inform nestmates of food location. On the other hand, wasps do learn the odor of food brought to the nest and use this cue to locate the food source outside the nest. Here, we quantify the effects of three food‐associated variables on the allocation of foraging effort in the yellowjacket Vespula germanica. We used an experimental approach to assess whether resource quantity, quality, or associated olfactory information affect the probability that a forager will leave the nest on a foraging trip. We addressed these questions by inserting a known amount of sucrose solution directly into nests and recording foraging effort (departure rate) over the subsequent hour‐long observation period. No differences were found in foraging effort because of the presence/absence of olfactory cues, but there was strong evidence that foraging effort increased in response to resource influx and resource quality. Thus, while olfactory cues are learned in the nest, only resource quality and the cue of increased amount of food in the nest factor into a forager's decision of whether or not to depart on a foraging trip. However, as prior work has shown, once a wasp forager leaves the nest, it uses the learned olfactory cues to aid in finding resources. 相似文献
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Andrew M. Bouwma Peter E. Bouwma Erik V. Nordheim Robert L. Jeanne 《Journal of Insect Behavior》2003,16(3):439-452
When colonies of swarm-founding wasps lose their nests to predation or accident, the entire adult population escapes, emigrates as an absconding swarm, and renests elsewhere. Such an event causes a reduction in the adult population due to losses during the emigration itself and to adult attrition without replacement during the subsequent preemergence growth period in the new nest. We addressed the first of these sources of mortality for 27 absconding swarms of Polybia occidentalis in Costa Rica. Adult mortality over the day that included swarm emigration averaged 0.044 ± 0.039 (SD) of the original population and was a weak positive function of distance moved, but not of swarm size. A larger data set showed that emigration distance increased with swarm size. This is the first study to measure mortality rates during emigration in a swarm-founding social insect. 相似文献
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David J. Mladenoff Sally E. Dahir Eric V. Nordheim Lisa A. Schulte Glenn G. Guntenspergen 《Ecosystems》2002,5(6):539-553
Historical data have increasingly become appreciated for insight into the past conditions of ecosystems. Uses of such data include assessing the extent of ecosystem change; deriving ecological baselines for management, restoration, and modeling; and assessing the importance of past conditions on the composition and function of current systems. One historical data set of this type is the Public Land Survey (PLS) of the United States General Land Office, which contains data on multiple tree species, sizes, and distances recorded at each survey point, located at half-mile (0.8-km) intervals on a 1-mi (1.6 km) grid. This survey method was begun in the 1790s on US federal lands extending westward from Ohio. Thus, the data have the potential of providing a view of much of the US landscape from the mid-1800s, and they have been used extensively for this purpose. However, historical data sources, such as those describing the species composition of forests, can often be limited in the detail recorded and the reliability of the data, since the information was often not originally recorded for ecological purposes. Forest trees are sometimes recorded ambiguously, using generic or obscure common names. For the PLS data of northern Wisconsin, USA, we developed a method to classify ambiguously identified tree species using logistic regression analysis, using data on trees that were clearly identified to species and a set of independent predictor variables to build the models. The models were first created on partial data sets for each species and then tested for fit against the remaining data. Validations were conducted using repeated, random subsets of the data. Model prediction accuracy ranged from 81% to 96% in differentiating congeneric species among oak, pine, ash, maple, birch, and elm. Major predictor variables were tree size, associated species, landscape classes indicative of soil type, and spatial location within the study region. Results help to clarify ambiguities formerly present in maps of historic ecosystems for the region and can be applied to PLS datasets elsewhere, as well as other sources of ambiguous historical data. Mapping the newly classified data with ecological land units provides additional information on the distribution, abundance, and associations of tree species, as well as their relationships to environmental gradients before the industrial period, and clarifies the identities of species formerly mapped only to genus. We offer some caveats on the appropriate use of data derived in this way, as well as describing their potential. 相似文献
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