全文获取类型
收费全文 | 1149篇 |
免费 | 85篇 |
出版年
2022年 | 6篇 |
2021年 | 24篇 |
2020年 | 10篇 |
2019年 | 12篇 |
2018年 | 19篇 |
2017年 | 11篇 |
2016年 | 29篇 |
2015年 | 31篇 |
2014年 | 36篇 |
2013年 | 39篇 |
2012年 | 77篇 |
2011年 | 115篇 |
2010年 | 85篇 |
2009年 | 57篇 |
2008年 | 40篇 |
2007年 | 58篇 |
2006年 | 51篇 |
2005年 | 36篇 |
2004年 | 43篇 |
2003年 | 29篇 |
2002年 | 36篇 |
2001年 | 30篇 |
2000年 | 33篇 |
1999年 | 23篇 |
1998年 | 9篇 |
1997年 | 11篇 |
1996年 | 7篇 |
1995年 | 9篇 |
1992年 | 17篇 |
1991年 | 18篇 |
1990年 | 19篇 |
1989年 | 11篇 |
1988年 | 15篇 |
1987年 | 10篇 |
1986年 | 7篇 |
1985年 | 18篇 |
1984年 | 7篇 |
1983年 | 8篇 |
1982年 | 10篇 |
1981年 | 7篇 |
1980年 | 7篇 |
1979年 | 8篇 |
1978年 | 12篇 |
1977年 | 10篇 |
1976年 | 7篇 |
1975年 | 9篇 |
1974年 | 9篇 |
1973年 | 13篇 |
1972年 | 6篇 |
1971年 | 8篇 |
排序方式: 共有1234条查询结果,搜索用时 46 毫秒
991.
992.
993.
Genetic and physical mapping around the properdin P gene. 总被引:6,自引:0,他引:6
M P Coleman J C Murray H F Willard K F Nolan K B Reid D J Blake S Lindsay S S Bhattacharya A Wright K E Davies 《Genomics》1991,11(4):991-996
A CA repeat has been found on the human X chromosome within 16 kb of the gene encoding properdin P factor (PFC) and has been shown to be a highly informative marker. Two more polymorphic CA repeats were found in a cosmid containing DXS228. The CA repeats, and other markers from proximal Xp, were mapped genetically in CEPH families and the likely order of markers was established as Xpter-(DXS7, MAO-A, DXS228)-(PFC, DXS426)-(TIMP, OATL1)-DXS255-Xcen. This places PFC in the region Xp11.3-Xp11.23, thus refining previous in situ hybridization data. Two yeast artificial chromosomes (YACs) (440 and 390 kb) contain both PFC and DXS426, and one of them (440 kb) also contains TIMP. This confirms the genetic order TIMP-(PFC, DXS426). PFC and TIMP are located on the same 100-kb SalI/PvuI fragment of the 440-kb YAC. Given the genetic orientation of TIMP and (PFC, DXS426), this YAC can now serve as a starting point for directional walking toward disease genes located in Xp11.3-Xp11.2 such as retinitis pigmentosa (RP2) and Wiskott-Aldrich syndrome. 相似文献
994.
The molecular basis of circadian homeostasis has proven to be amenable to genetic dissection in many model organisms. Surprisingly,
additional factors contributing to an organism’s “chronotype” continue to be identified using both forward and reverse genetics.
As more factors are identified, the importance of rhythm regulation in all body systems is becoming apparent. Moreover, recent
evidence confirms that the regulation of circadian homeostasis can be fine-tuned at a number of molecular levels. This not
only ensures that biological rhythms are maintained at a robust level in all cells but also allows for the precise and rapid
readjustment of rhythms in response to environmental factors. 相似文献
995.
Margarita Vigodner Jeffrey H. Weisburg Vibha Shrivastava Rebecca A. Marmor Jennifer Fathy Nolan Skop 《Cell and tissue research》2009,336(2):277-286
Small ubiquitin-like modifier (SUMO) proteins are involved in a variety of cellular processes. Alterations in SUMO conjugation
have been implicated in several human diseases, including cancer. Although the main cause of failure in cancer treatment is
the development of drug resistance by cancer cells, the mechanisms of drug resistance are not fully understood. SUMO proteins
are thought to play roles in various cellular pathways, but no studies have as yet compared the expression of the different
SUMO proteins in chemosensitive and drug-resistant cancer cells. To determine the relationship between protein sumoylation
and drug resistance, the expression of various SUMO isoforms has been studied and compared in the HL-60 cell line (a model
for leukemic cells) and in HL-60RV cells (resistant to vincristine). Co-immunostaining of cells by anti-SUMO antibodies and
antibodies against various nuclear subdomains has been examined by an advanced type of bioimaging analysis. Whereas SUMO-2/3
co-localizes exclusively with nuclear bodies containing promyelocytic leukemia protein in both cell types, SUMO-1 has also
been seen in nucleolar regions of HL-60, but not in HL-60RV, cells. In HL-60 cells, SUMO-1 occurs adjacent to, but not co-localized
with, the nucleolar marker fibrillarin. Western blot analysis has revealed higher levels of free SUMO and sumoylated products
in drug-resistant cells and the presence of specific SUMO-1 conjugates in drug-sensitive HL-60 cells, possibly consistent
with a specific nucleolar signal. Shortly after the induction of ethanol and oxidative stress, HL-60RV, but not HL-60, cells
show increased accumulation of high-molecular-weight SUMO-2/3 conjugates. Thus, SUMO-1 probably has a specific role in the
nucleoli of HL-60 cells, and the alteration of sumoylation might be a contributing factor in the development of drug resistance
in leukemia cells.
The author thanks Stern College for Women, Yeshiva University and the Joseph Alexander Foundation for supporting this research
project. 相似文献
996.
Tindall BJ Schneider S Lapidus A Copeland A Glavina Del Rio T Nolan M Lucas S Chen F Tice H Cheng JF Saunders E Bruce D Goodwin L Pitluck S Mikhailova N Pati A Ivanova N Mavrommatis K Chen A Palaniappan K Chain P Land M Hauser L Chang YJ Jeffries CD Brettin T Han C Rohde M Göker M Bristow J Eisen JA Markowitz V Hugenholtz P Klenk HP Kyrpides NC Detter JC 《Standards in genomic sciences》2009,1(3):270-277
Halomicrobium mukohataei (Ihara et al. 1997) Oren et al. 2002 is the type species of the genus Halomicrobium. It is of phylogenetic interest because of its isolated location within the large euryarchaeal family Halobacteriaceae. H. mukohataei is an extreme halophile that grows essentially aerobically, but can also grow anaerobically under a change of morphology and with nitrate as electron acceptor. The strain, whose genome is described in this report, is a free-living, motile, Gram-negative euryarchaeon, originally isolated from Salinas Grandes in Jujuy, Andes highlands, Argentina. Its genome contains three genes for the 16S rRNA that differ from each other by up to 9%. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first completed genome sequence from the poorly populated genus Halomicrobium, and the 3,332,349 bp long genome (chromosome and one plasmid) with its 3416 protein-coding and 56 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
997.
Copeland A Lapidus A Glavina Del Rio T Nolan M Lucas S Chen F Tice H Cheng JF Bruce D Goodwin L Pitluck S Mikhailova N Pati A Ivanova N Mavromatis K Chen A Palaniappan K Chain P Land M Hauser L Chang YJ Jeffries CD Chertkov O Brettin T Detter JC Han C Ali Z Tindall BJ Göker M Bristow J Eisen JA Markowitz V Hugenholtz P Kyrpides NC Klenk HP 《Standards in genomic sciences》2009,1(2):119-125
Catenulispora acidiphila Busti et al. 2006 is the type species of the genus Catenulispora, and is of interest because of the rather isolated phylogenetic location it occupies within the scarcely explored suborder Catenulisporineae of the order Actinomycetales. C. acidiphilia is known for its acidophilic, aerobic lifestyle, but can also grow scantly under anaerobic conditions. Under regular conditions, C. acidiphilia grows in long filaments of relatively short aerial hyphae with marked septation. It is a free living, non motile, Gram-positive bacterium isolated from a forest soil sample taken from a wooded area in Gerenzano, Italy. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first complete genome sequence of the actinobacterial family Catenulisporaceae, and the 10,467,782 bp long single replicon genome with its 9056 protein-coding and 69 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
998.
Lang E Lapidus A Chertkov O Brettin T Detter JC Han C Copeland A Glavina Del Rio T Nolan M Chen F Lucas S Tice H Cheng JF Land M Hauser L Chang YJ Jeffries CD Kopitz M Bruce D Goodwin L Pitluck S Ovchinnikova G Pati A Ivanova N Mavrommatis K Chen A Palaniappan K Chain P Bristow J Eisen JA Markowitz V Hugenholtz P Göker M Rohde M Kyrpides NC Klenk HP 《Standards in genomic sciences》2009,1(2):133-140
Dyadobacter fermentans (Chelius and Triplett, 2000) is the type species of the genus Dyadobacter. It is of phylogenetic interest because of its location in the Cytophagaceae, a very diverse family within the order 'Sphingobacteriales'. D. fermentans has a mainly respiratory metabolism, stains Gram-negative, is non-motile and oxidase and catalase positive. It is characterized by the production of cell filaments in aging cultures, a flexirubin-like pigment and its ability to ferment glucose, which is almost unique in the aerobically living members of this taxonomically difficult family. Here we describe the features of this organism, together with the complete genome sequence, and its annotation. This is the first complete genome sequence of the sphingobacterial genus Dyadobacter, and this 6,967,790 bp long single replicon genome with its 5804 protein-coding and 50 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
999.
Han C Sikorski J Lapidus A Nolan M Glavina Del Rio T Tice H Cheng JF Lucas S Chen F Copeland A Ivanova N Mavromatis K Ovchinnikova G Pati A Bruce D Goodwin L Pitluck S Chen A Palaniappan K Land M Hauser L Chang YJ Jeffries CD Chain P Saunders E Brettin T Göker M Tindall BJ Bristow J Eisen JA Markowitz V Hugenholtz P Kyrpides NC Klenk HP Detter JC 《Standards in genomic sciences》2009,1(3):226-233
Kangiella koreensis (Yoon et al. 2004) is the type species of the genus and is of phylogenetic interest because of the very isolated location of the genus Kangiella in the gammaproteobacterial order Oceanospirillales. K. koreensis SW-125(T) is a Gram-negative, non-motile, non-spore-forming bacterium isolated from tidal flat sediments at Daepo Beach, Yellow Sea, Korea. Here we describe the features of this organism, together with the complete genome sequence, and annotation. This is the first completed genome sequence from the genus Kangiella and only the fourth genome from the order Oceanospirillales. This 2,852,073 bp long single replicon genome with its 2647 protein-coding and 48 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
1000.
Ivanova N Sikorski J Sims D Brettin T Detter JC Han C Lapidus A Copeland A Glavina Del Rio T Nolan M Chen F Lucas S Tice H Cheng JF Bruce D Goodwin L Pitluck S Pati A Mavromatis K Chen A Palaniappan K D'haeseleer P Chain P Bristow J Eisen JA Markowitz V Hugenholtz P Göker M Pukall R Klenk HP Kyrpides NC 《Standards in genomic sciences》2009,1(2):110-118
Sanguibacter keddieii is the type species of the genus Sanguibacter, the only genus within the family of Sanguibacteraceae. Phylogenetically, this family is located in the neighborhood of the genus Oerskovia and the family Cellulomonadaceae within the actinobacterial suborder Micrococcineae. The strain described in this report was isolated from blood of apparently healthy cows. Here we describe the features of this organism, together with the complete genome sequence, and annotation. This is the first complete genome sequence of a member of the family Sanguibacteraceae, and the 4,253,413 bp long single replicon genome with its 3735 protein-coding and 70 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献