全文获取类型
收费全文 | 832篇 |
免费 | 33篇 |
出版年
2021年 | 7篇 |
2020年 | 4篇 |
2018年 | 4篇 |
2017年 | 9篇 |
2016年 | 13篇 |
2015年 | 24篇 |
2014年 | 27篇 |
2013年 | 48篇 |
2012年 | 37篇 |
2011年 | 45篇 |
2010年 | 15篇 |
2009年 | 25篇 |
2008年 | 46篇 |
2007年 | 51篇 |
2006年 | 50篇 |
2005年 | 53篇 |
2004年 | 56篇 |
2003年 | 39篇 |
2002年 | 27篇 |
2001年 | 8篇 |
2000年 | 13篇 |
1999年 | 9篇 |
1998年 | 9篇 |
1997年 | 10篇 |
1996年 | 3篇 |
1995年 | 7篇 |
1994年 | 5篇 |
1993年 | 5篇 |
1992年 | 15篇 |
1991年 | 14篇 |
1990年 | 11篇 |
1989年 | 11篇 |
1988年 | 24篇 |
1987年 | 11篇 |
1986年 | 13篇 |
1985年 | 9篇 |
1984年 | 9篇 |
1983年 | 12篇 |
1982年 | 7篇 |
1981年 | 11篇 |
1980年 | 8篇 |
1979年 | 7篇 |
1978年 | 7篇 |
1977年 | 5篇 |
1976年 | 4篇 |
1975年 | 5篇 |
1974年 | 4篇 |
1973年 | 4篇 |
1970年 | 4篇 |
1966年 | 4篇 |
排序方式: 共有865条查询结果,搜索用时 15 毫秒
11.
Effect of proteinase inhibitors on intracellular processing of cathepsin B, H and L in rat macrophages 总被引:2,自引:0,他引:2
The effects of various proteinase inhibitors on the processing of lysosomal cathepsins B, H and L were investigated in cultured rat peritoneal macrophages. The processing of newly synthesized pro-cathepsins B, H and L to the mature single-chain enzymes was sensitive to a metal chelator,1,10-phenanthroline, and a synthetic metalloendopeptidase substrate, Z-Gly-Leu-NH2, and insensitive to inhibitors of serine proteinases, aspartic proteinases and cysteine proteinases. Inhibitors of cysteine proteinases, E-64-d and leupeptin, inhibited the processing of the single-chain forms of cathepsins B, H and L to the two-chain forms. These results suggest that (a) metal endopeptidase(s) is (are) involved in the propeptide processing of cathepsin B, H and L, and that proteolytic cleavages of the mature single-chain cathepsins are accomplished by cysteine proteinases in lysosomes. 相似文献
12.
Inhibition of cathepsin L-induced degradation of epidermal growth factor receptors by c-Ha-ras gene products 总被引:1,自引:0,他引:1
T Hiwasa S Sakiyama S Yokoyama J M Ha J Fujita S Noguchi Y Bando E Kominami N Katunuma 《Biochemical and biophysical research communications》1988,151(1):78-85
The inhibitory activities of c-Ha-ras gene products (p21s) toward several cysteine proteinases have been investigated. The activity of cathepsin L was inhibited by p21s most effectively while those of cathepsin B and papain were slightly inhibited by p21s. p21s did not show any inhibitory activity toward cathepsin H. In order to connect the protease-inhibitor activity of p21s with cell growth, the degradation of epidermal growth factor receptors (EGF-receptors) was investigated. EGF-receptors were preferentially cleaved by cathepsin L but not by cathepsin B or H. The cleavage of EGF-receptors by cathepsin L was inhibited by p21s dose-dependently. These results raise the possibility that p21s can suppress the degradation of growth-related proteins such as EGF-receptors and thereby affect cell growth. 相似文献
13.
A chymotrypsin-type serine protease in rat basophilic leukemia cells: evidence for its immunologic identity with atypical mast cell protease 总被引:2,自引:0,他引:2
H Kido K Izumi H Otsuka N Fukusen Y Kato N Katunuma 《Journal of immunology (Baltimore, Md. : 1950)》1986,136(3):1061-1065
Activity of a chymotrypsin-type serine protease was found in a subline of rat basophilic leukemia (RBL-2H3) cells. The protease was immunologically cross-reactive with anti-atypical mast cell protease immunoglobulin (Ig) G, and its activity was inhibited in a dose-dependent manner by the antibody. The apparent m.w. of the protease that reacted with the antibody was 25,000, which was identical with that of atypical mast cell protease in rat mucosal mast cells. These results show that the chymotrypsin type serine protease in RBL-2H3 cells is immunologically identical with atypical mast cell protease, which was first purified from rat small intestine. Immunohistochemical studies showed that the protease was located not only in intracytoplasmic granules but also in organelles synthesizing protein, such as cisternae of the rough endoplasmic reticulum, perinuclear spaces, and the Golgi apparatus. However, no immunoreactivity was demonstrated in rat basophils. The activity of the protease increased in the exponential phase of growth of RBL-2H3 cells in which some activity was also detected in the medium, and it decreased in the late stationary phase. 相似文献
14.
gamma-Glutamyltranspeptidase purified from human kidneys contains 4-5 asparagine-linked sugar chains in each molecule. The sugar chains were released from the polypeptide portion of the enzyme by hydrazinolysis as oligosaccharides and separated by paper electrophoresis into one neutral and two acidic fractions. By sequential exoglycosidase digestion and methylation analysis, the neutral fraction, which comprised 69% of total oligosaccharides, was shown to be a mixture of bisected bi- and triantennary complex-type sugar chains with and without a fucose on the proximal N-acetylglucosamine residue and with Gal beta 1----4GlcNAc and/or Gal beta 1----4(Fuc alpha 1----3)GlcNAc groups in their outer chain moieties. The acidic oligosaccharide fractions were mixtures of mono- and disialyl derivatives of bisected triantennary complex-type oligosaccharides with Gal beta 1----4GlcNAc and/or Gal beta 1----4(Fuc alpha 1----3)GlcNAc group in their outer chain moieties. Some of the outer chains of the acidic oligosaccharides were considered to be sialylated X-antigenic structures. 相似文献
15.
K Ii K Hizawa E Kominami Y Bando N Katunuma 《The journal of histochemistry and cytochemistry》1985,33(11):1173-1175
Different localizations of cathepsin B, H, and L in normal rat liver were revealed immunohistochemically with anticathepsin Fab'-horseradish peroxidase conjugates. Staining of cathepsin B was strong in the periportal sinusoids, possibly in Kupffer cells; and weaker in panlobular hepatocytes. Staining of cathepsin H was strong in panlobular hepatocytes, especially in the periphery of the cytoplasm, possibly representing the peribiliary dense bodies; and weaker in periportal sinusoidal cells, possibly Kupffer cells. Staining of cathepsin L was strongest in centrilobular hepatocytes and weaker in periportal sinusoidal cells, possibly Kupffer cells. These findings, revealed for the first time in the present study, show that the histologic and intracellular localizations of the three cathepsins are different, suggesting that they have different roles in degradation of exogenous and endogenous proteins. 相似文献
16.
Kaoru Miyazaki Keisuke Mashima Nobuhiko Yamashita Jinpei Yamashita Takekazu Horio 《In vitro cellular & developmental biology. Plant》1985,21(1):62-66
Summary We have previously reported the transformation by Rous sarcoma virus of a cloned epithelial cell line (BRL) established from
Buffalo rat liver by H. Coon. The nontransformed (BRL) and transformed (RSV-BRL) cells grew at comparable rates in culture,
whereas only the transformed cells were tumorigenic in vivo. We report here on the existence in rat and mouse sera of a growth
inhibitor for the nontransformed BRL cells. The transformed BRL cells (RSV-BRL) were insensitive to this inhibitor. The inhibitory
activity was not prominent in sera from other species of animals tested except for rabbit; this serum inhibited the growth
of RSV-BRL cells more strongly than that of BRL cells. The growth inhibitor was partially purified from rat serum. It is a
protein free of lipid and has a molecular weight of about 220 000. The inhibitor could be separated into three components
of pI 4.6, 5.2 (major) and 5.6 by isoelectric electrophoresis.
EDITOR'S STATEMENT Although compelling theoretical arguments sometimes can be made for the likely existence of growth-inhibitory
substances of physical relevance in the control of cell proliferation, experiments aimed at identifying and studying such
factors often are difficult to design and interpret, and little strong data exists to suggest that growth-inhibitory substances
are important regulatorsin vivo. The information presented in this paper represents a start toward developing a useful system for studying growth-inhibitory
factor. David W. Barnes 相似文献
17.
Nobuhiko Saitô 《Cell biochemistry and biophysics》1987,11(1):321-329
The metling behavior of DNA and formation of α-helices and β-strands in protein can be discussed rigorously by statistical mechanical treatment. To do this, however, appropriate formulations are required for fast computer calculation. New formalisms for DNA and protein in terms of recurrence relations suitable for computer calculation are presented. 相似文献
18.
Among several intracellular protease tested, cathepsin H transformed leukotriene D4 to E4 with a release of glycine in a stoichiometric quantity. Under the optimal conditions the rate of leukotriene D4 transformation by cathepsin H was about 3% of the hydrolysis rate of alpha-N-benzoyl-DL-arginine-2-naphthylamide which is commonly utilized as a very efficient substrate to test the peptidase activity of the enzyme. Leukotriene C4 was not transformed to leukotriene D4 by cathepsin H. Neither cathepsin B nor C was active with leukotrienes C4 and D4. 相似文献
19.
20.