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31.
32.
ABSTRACT. Cell division in higher eukaryotes is mainly controlled by CDK (serine/threonine) protein kinases and by other components of these kinase complexes. Previously, we showed homologous kinases also occur in the ciate Paramecium tetraurelia . In this report, a polyclonal antibody was produced against a GST fusion protein which contained the N-terminal third of the previously isolated CDC2FTA protein. It recognized a 36 kDa polypeptide on western blots and did not cross-react with the related 34 kDa polypeptide. This 36 kDa polypeptide showed no affinity for yeast P13suc1 protein. The CDC2PTA level was invariant throughout the cell cycle and decreased slightly when cells entered stationary phase. Indirect immunofluorescence showed CDC2PTA localized in the macronucleus, but not in micronuclei. Native CDC2FTA was immunoprecipitated and the kinase activity was assayed using histone HI as substrate in elutriation synchronized samples. The kinase activity was high during the early stages of the cell cycle and reached a peak at 2.5 h after elutriation, which corresponds to the time of initiation of macronuclear DNA synthesis. This suggests CDC2PTA kinase may be associated with the regulation of macronuclear DNA synthesis. These results allow us to draw concrete comarisons for the first time between the role of CDK in ciliates and higher eukaryotes. 相似文献
33.
Near the end of the cell cycle, ciliates commit irreversibly to cell division. The point of commitment occurs at the time of oral polykinetid assembly and micronuclear anaphase. The commitment is a checkpoint which requisites a threshold cell mass/ DNA ratio and stomatogenesis. It is also a physiological transition point, involving cdk protein kinases similar to those of other eukaryotes. Both P34 kD and P36 kD kinases, similar to the S. pombe cdc2 kinases, have been described to have activity as monomers. Subsequent to commitment to division, dramatic cytoskeletal modifications occur for separation of organelles, cortex morphogenesis and cytokinesis. Numerous mutants affecting cytoskeletal function associated with the division process have been obtained in several species. Of these, only the ccl mutant in Paramecium affects cell cycle progression prior to commitment to division. The material reviewed is used to speculate about the mechanisms of regulation of pre-fission morphogenesis and cell division related processes in ciliates. 相似文献
34.
The morphology and morphogenesis of the kinetofragminophoran soil ciliates, Fuscheria terricola n. sp. and Spathidium muscorum Dragesco & Dragesco-Kerneis, 1979, are described. Stained specimens (protargol) are characterized biometrically. The new species differs from the other species of the genus in its body size, body shape, number of kineties, length of extrusomes, and habitat. Both species have telokinetal stomatogenesis, which commences with a proliferation of kinetosomes at those kineties which bear the brosse. Fuscheria terricola does not have a complex perioral ciliature; indeed, it might be that this species has only monokinetids. Thus only a proliferation of kinetosomes and the separation of the kineties takes place in the prospective division furrow. In contrast, S. muscorum differentiates short dikinetid kinetofragments in the region of the division furrow, which are arranged to form the perioral kinety of the opisthe in the intermediate and late stages of the stomatogenesis. The right part of the perioral kinety develops first. This and other studies show that telokinetal stomatogenesis proceeds very differently depending on the differentiation of the oral ciliature; however, detailed studies on the morphogenesis of kinetofragminophoran ciliates are still too few in number for subtypes to be defined. 相似文献
35.
36.
RAINER BERGER 《American anthropologist》1975,77(4):910-911
Book reviewed in this article:
LITHIC TECHNOLOGY FILMS: A Tale of Two Rivers . Produced by CLYDE B. SMITH.
LITHIC TECHNOLOGY FILMS: Lascaux: Cradle of Man's Art . Directed by WILLIAM C. CHAPMAN.
LITHIC TECHNOLOGY FILMS: Prehistoric Images . 1955. Directed by THOMAS L. ROWE 相似文献
LITHIC TECHNOLOGY FILMS: A Tale of Two Rivers . Produced by CLYDE B. SMITH.
LITHIC TECHNOLOGY FILMS: Lascaux: Cradle of Man's Art . Directed by WILLIAM C. CHAPMAN.
LITHIC TECHNOLOGY FILMS: Prehistoric Images . 1955. Directed by THOMAS L. ROWE 相似文献
37.
T. Rütting H. Brandt W. Clauβ V. Dzapo D. Selzer 《European Journal of Wildlife Research》2007,53(3):171-177
The aim of the study was to determine differences in the behaviour of chicks of three different pheasant subspecies. The timidity
of the pheasant chicks was of special interest, therefore behaviour traits were analysed using an open-field test and a tonic
immobility test. In total, 137 pheasant chicks were tested including the copper pheasant (Ph. c. colchicus; n = 87), the green pheasant (Ph. c. versicolor; n = 12) and the melanistic pheasant (Ph. c. tenebrosus; n = 38). In both tests, the chicks of the versicolor group showed the highest timidity, whereas the other two groups were similar
in timidity. In the open-field test, the chicks of the colchicus group showed the lowest timidity, and in the tonic immobility
test, the tenebrosus group showed the lowest values. Assuming a relationship between the test results and predator avoidance
behaviour, it can be concluded that the chicks of the versicolor group show the best predator avoidance behaviour. Further
investigations are needed to find out whether the differences in the analysed behaviour traits result in higher survival rates
after releasing to the wild. 相似文献
38.
Tolerance in banana to Fusarium wilt is associated with early up-regulation of cell wall-strengthening genes in the roots 总被引:1,自引:0,他引:1
NOËLANI VAN DEN BERG DAVE K. BERGER INGO HEIN PAUL R. J. BIRCH MICHAEL J. WINGFIELD ALTUS VILJOEN 《Molecular Plant Pathology》2007,8(3):333-341
Fusarium wilt, caused by the fungal pathogen Fusarium oxysporum f. sp. cubense ( Foc ), is one of the most destructive diseases of bananas. In the tropics and subtropics, Cavendish banana varieties are highly susceptible to Foc race 4 (VCG 0120). Cavendish selection GCTCV-218 was shown to have significantly lower disease severity and incidence compared with susceptible cultivar Williams in replicated greenhouse and field trials. Suppression subtractive hybridization (SSH) was previously carried out to identify genes induced in roots of GCTCV-218, but not in Williams, after infection with Foc 'subtropical' race 4 . Seventy-nine SSH clones were sequenced and revealed 13 non-redundant gene fragments, several of which showed homology to defence-associated genes, including cell wall-strengthening genes. Quantitative RT-PCR was used to confirm up-regulation and differential expression of a number of genes throughout a time-course, following Foc infection in the tolerant GCTCV-218 when compared with susceptible cv. Williams . Tolerance of GCTCV-218 was linked to significantly increased induction of cell wall-associated phenolic compounds. 相似文献
39.
J. Fiβler C. Schirra G. -W. Kohring F. Giffhorn 《Applied microbiology and biotechnology》1994,41(4):395-399
The capability of five strains of the phototrophic bacteriumRhodopseudomonas palustris to produce molecular hydrogen (H2) from the aromatic acids benzoate,p-hydroxybenzoate, cinnamate and D- and L-mandelate was investigated. Optimal H2 production was achieved when the strains were grown anaerobically in the light at 10,000 lx under nitrogen (N) limitation using 1 mM L-glutamate as an N source. In the presence of 2 mM benzoate or L-mandelate as carbon and electron sources, strain DSM 131 produced 45% H2 of the maximal theoretical value and strain F2 32%, respectively. Increased H2 production correlated with increased nitrogenase activities, but H2 formation was not further stimulated by inhibition of the H2 uptake (hup) hydrogenase with ethylenediaminetetraacetic acid (EDTA). 相似文献
40.
LIREN TANG STEVEN L. PELECH JAMES D. BERGER 《The Journal of eukaryotic microbiology》1994,41(4):381-387
ABSTRACT. Cell division in higher eukaryotes is mainly controlled by p34 cdc2 or related kinases and by other components of these kinase complexes. We present evidence that cdc2 -like kinases also occur in Paramecium. Two polypeptides reacted with an antibody directed against the perfectly conserved PSTAIR region found in cdc2 kinases in other eukaryotes. Only the less abundant peptide bound to p13 suc1 from Schizosaccharomyces pombe. Using centrifugal elutriation to select cells on the basis of size, we isolated highly synchronous Paramecium G1 cells. With this procedure, we demonstrated that the p13suc1 -associated cdc2 -like histone H1 kinase was activated before cell division at the point of commitment to division in Paramecium. Further, we show that Paramecium cdc2 -like proteins occurred principally as monomers and that these monomers were active as histone H1 kinases in vitro. 相似文献