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31.
32.
Identification of a strawberry gene encoding a non-specific lipid transfer protein that responds to ABA,wounding and cold stress 总被引:6,自引:0,他引:6
Yubero-Serrano EM Moyano E Medina-Escobar N Muñoz-Blanco J Caballero JL 《Journal of experimental botany》2003,54(389):1865-1877
cDNA and genomic clones encoding a strawberry (Fragariaxananassa cv. Chandler) non-specific lipid transfer protein (Fxaltp gene) were isolated and characterized. The spatio-temporal expression pattern and structural features of this gene were studied for the first time in strawberry, a non-climacteric fruit of agricultural importance. The architecture and the encoded amino acid sequence of this non-climacteric fruit ltp gene were similar to those of other plant LTPs previously reported, and presents the eight cysteine residues and other features characteristic of plant LTPs. In addition, the deduced protein posseses an N-terminal signal peptide and lacks the K/HDEL retention signal, indicating that the strawberry LTP protein would enter the secretory pathway. In situ studies have shown that the Fxaltp gene is expressed in the epidermal cell layer of the strawberry fruit receptacle and achenes, flowers, and within the cell layer surrounding the endosperm. These results suggest that this Fxaltp gene promoter could be used as an endogenous promoter for biotechnological purposes in strawberry. Computer analysis using the PLACE database predicted the presence of several putative cis-regulatory sequences in response to abscisic acid and cold or wounding stresses within the Fxaltp 5'-flanking region. Accordingly, the strawberry gene responds to ABA and SA, but not to salt and heat stresses. It is also reported that ltp gene expression in strawberry is stimulated by wounding and repressed by cold stresses. 相似文献
33.
Wang F Miles RW Kicska G Nieves E Schramm VL Angeletti RH 《Protein science : a publication of the Protein Society》2000,9(9):1660-1668
The rate and extent of hydrogen/deuterium (H/D) exchange into purine nucleoside phosphorylase (PNP) was monitored by electrospray ionization mass spectrometry (ESI-MS) to probe protein conformational and dynamic changes induced by a substrate analogue, products, and a transition state analogue. The genetic deficiency of PNP in humans is associated with severe T-cell immunodeficiency, while B-cell immunity remains functional. Inhibitors of PNP have been proposed for treatment of T-cell leukemia, to suppress the graft-vs.-host response, or to counter type IV autoimmune diseases without destroying humoral immunity. Calf spleen PNP is a homotrimer of polypeptide chains with 284 amino residues, molecular weight 31,541. Immucillin-H inhibits PNP with a Kd of 23 pM when only one of the three catalytic sites is occupied. Deuterium exchange occurs at 167 slow-exchange sites in 2 h when no catalytic site ligands are present. The substrate analogue and product prevented H/D exchange at 10 of the sites. Immucillin-H protected 32 protons from exchange at full saturation. When one of the three subunits of the homotrimer is filled with immucillin-H, and 27 protons are protected from exchange in all three subunits. Deuterium incorporation in peptides from residues 132-152 decreased in all complexes of PNP. The rate and/or extent of deuterium incorporation in peptides from residues 29-49, 50-70, 81-98, and 112-124 decreased only in the complex with the transition state analogue. The peptide-specific H/D exchange demonstrates that (1) the enzyme is most compact in the complex with immucillin-H, and (2) filling a single catalytic site of the trimer reduces H/D exchange in the same peptides in adjacent subunits. The peptides most highly influenced by the inhibitor surround the catalytic site, providing evidence for reduced protein dynamic motion caused by the transition state analogue. 相似文献
34.
R.A. Nieves C.I. Ehrman W.S. Adney R.T. Elander M.E. Himmel 《World journal of microbiology & biotechnology》1998,14(2):301-304
Commercial cellulase enzymes have been used in the food, detergent, and textile industries, and are potentially effective for processing biomass feedstocks. A survey was undertaken to identify major manufacturers/distributors of cellulases in the USA and to evaluate 13 representative commercial preparations for enzyme activity, protein concentration, and chemical composition. Samples were subjected to activity measurements using filter paper, carboxymethylcellulose, cellobiose, and p-nitrophenyl--d-glucopyranoside as substrates. To ascertain the microbial origin of the commercial preparations, Western blots utilizing monoclonal antibodies specific for Trichoderma reesei CBH I and Aspergillus niger -d-glucosidase were developed. Eleven of the cellulases tested were of T. reesei or T. viride origin and two were from A. niger. 相似文献
35.
Artificial endosperm of Cleopatra tangerine zygotic embryos: a model for somatic embryo encapsulation 总被引:1,自引:0,他引:1
Nieves Nadina Lorenzo Jose C. Blanco Maria de los A. González Justo Peralta Hipólito Hernández Martha Santos Ramón Concepción Oscar Borroto Carlos G. Borroto Eduviges Tapia Raúl Martinez Marcos E. Fundora Zaida González Alfredo 《Plant Cell, Tissue and Organ Culture》1998,54(2):77-83
Synthetic seed technology may be of value in breeding programs and allow the propagation of many elite genotype-derived plants
in a short time. In this work, a range of artificial endosperm treatments of Cleopatra tangerine zygotic embryos were evaluated
for suitability for encapsulation of somatic embryos. Different complexing ions in the form of alginate capsules, zeolite
as an ion exchanger and the relationship between capsule-nutrient gel on germination of zygotic embryos, were evaluated. Artificial
endosperm assays showed that abscisic acid (1 μM) and mannitol (0.25 M) delayed germination and conversion of zygotic embryos,
whereas amino acid supplements (proline, glutamic acid and arginine) accelerated the conversion process. An artificial endosperm
was used to encapsulate somatic and zygotic embryos. After encapsulation, zygotic embryos germinated after four days of culture
while somatic embryos germinated asynchronously after 20 days. Somatic embryo-derived plantlets showed greater vigour than
zygotic embryo-derived plantlets. Results showed that this artificial endosperm is adequate for Cleopatra tangerine somatic
embryo germination and conversion into plants.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
36.
María G. Barderas José Tuñón Verónica M. Dardé Fernando De la Cuesta José J. Jiménez‐Nácher Nieves Tarín Lorenzo López‐Bescós Jesús Egido Fernando Vivanco Professor 《Proteomics》2009,9(7):1982-1993
Aggressive treatment with high‐dose atorvastatin reduces more effectively the incidence of cardiovascular events than moderate statin therapy. The mechanism of this benefit has not been fully elucidated. In order to know the potential effects of statin treatment on the protein expression of circulating monocytes in acute coronary syndrome (ACS) patients, a proteomic analysis of these cells was carried out by 2‐DE and MS. Twenty‐five patients with non‐ST‐elevation acute coronary syndrome (NSTEACS) were randomized, the fourth day after admission, to receive ATV 80 mg/dL (n = 14) or conventional treatment (CT) (n = 11), for two months. Blood was withdrawn at the end of the treatment, and monocytes were extracted for proteomic analysis and their protein expression patterns determined. Age, sex, total cholesterol, LDL, HDL, triglycerides, body mass index, presence of hypertension, diabetes, and smoking status were not significantly different between the two groups of patients. The expression of 20 proteins was modified by intensive ATV. Among the most relevant results stand out the normalization by intensive ATV treatment of the expression of proteins that modulate inflammation and thrombosis such as protein disulfide isomerase ER60 (PDI), Annexin I, and prohibitin, or that have other protective effects as HSP‐70. Thus, this approach shed light at the molecular level of the beneficial mechanisms of anti‐atherothrombotic drugs. 相似文献
37.
Javier Quinteiro Pablo Manent Lois Pérez-Diéguez José A. González Corrine Almeida Evandro Lopes Ricardo Araújo Gilberto P. Carreira Manuel Rey-Méndez Nieves González-Henríquez 《PloS one》2015,10(4)
The Azorean barnacle, Megabalanus azoricus (Pilsbry, 1916), is a Macaronesian endemic whose obscure taxonomy and the unknown relationships among forms inhabiting isolated Northern Atlantic oceanic islands is investigated by means of molecular analysis herein. Mitochondrial data from the 16S rRNA and COX1 genes support its current species status, tropical ancestry, and the taxonomic homogeneity throughout its distribution range. In contrast, at the intraspecific level and based on control region sequences, we detected an overall low level of genetic diversity and three divergent lineages. The haplogroups α and γ were sampled in the Azores, Madeira, Canary, and Cabo Verde archipelagos; whereas haplogroup β was absent from Cabo Verde. Consequently, population analysis suggested a differentiation of the Cabo Verde population with respect to the genetically homogenous northern archipelagos generated by current oceanographic barriers. Furthermore, haplogroup α, β, and γ demographic expansions occurred during the interglacial periods MIS5 (130 Kya - thousands years ago -), MIS3 (60 Kya), and MIS7 (240 Kya), respectively. The evolutionary origin of these lineages is related to its survival in the stable southern refugia and its demographic expansion dynamics are associated with the glacial-interglacial cycles. This phylogeographic pattern suggests the occurrence of genetic discontinuity informative to the delimitation of an informally defined biogeographic entity, Macaronesia, and its generation by processes that delineate genetic diversity of marine taxa in this area. 相似文献
38.
Félix Hernández Elena Langa Raquel Cuadros Jesús Avila Nieves Villanueva 《Molecular and cellular biochemistry》2010,344(1-2):211-215
Dephosphorylation of phospho GSK3 isoforms, from COS-7 cells, was determined in vitro and in cultured cells in the absence or the presence of okadaic acid and lithium. Our results indicate a preferential dephosphorylation of phospho GSK3α by PP2A phosphatase, whereas dephosphorylation of phospho GSK3β mainly takes place by PP1 phosphatase. 相似文献
39.
Iván Closa Juan José Irigoyen Nieves Goicoechea 《Trees - Structure and Function》2010,24(6):1029-1043
Beech forests naturally regenerating from clear-cutting can exhibit different microclimates depending on size of saplings
and stem density. When beech trees are young and stem density is low, the level of radiation inside the ecosystem reaching
the soil surface is high; consequently, air and soil temperatures rise and the soil water content may decrease. These microclimatic
parameters presumably will affect the anatomy, photosynthesis, and carbon metabolism of beech leaves. We studied the morphology
and physiology of sun and shade leaves of beech trees differing in age and growing within clear-cut areas with distinct microclimate.
Results were compared with those of adult trees in an unmanaged forest. We selected a stand clear-cut in 2001 (14,000 trees
ha−1), another clear-cut in 1996 (44,000 trees ha−1) and an unmanaged forest (1,000 trees ha−1). Photosynthetic photon flux density (PPFD) incident on sun leaves, air temperature, soil moisture, and soil temperature
within the forests affected water status and carbohydrate storage in all trees. As trees became older, PPFD also influenced
pigment composition and Rubisco activity in sun leaves. On the other hand, shade leaves from the oldest trees were the most
sensitive to PPFD, air temperature, and soil moisture and temperature inside the forest. Contrariwise, microclimatic parameters
slightly affected the physiology of shade leaves of the beech in the stand with the highest light attenuation. Air and soil
temperatures were the parameters that most affected the photosynthetic pigments and carbohydrate storage in shade leaves of
the youngest trees. 相似文献
40.
Fernández-Arrojo L Guazzaroni ME López-Cortés N Beloqui A Ferrer M 《Current opinion in biotechnology》2010,21(6):725-733
Microbial enzymes have many known applications as biocatalysts. However, only a few of them are currently employed for biocatalysis even though an annotated collection of more than 190 billion bases is available in metagenome sequence databases from uncultured and highly diverse microbial populations. This review aims at providing conceptual and technical bases for the translation of metagenome data into both experimental and computational frameworks that facilitates a comprehensive analysis of the biocatalysts diversity space. We will also briefly present the status of the current capabilities that assess and predict catalytic potential of environmental sites and track its diversity and evolution in large-scale biocatalysis process resulting from studies applying metagenomics in association with gene fingerprinting, catabolic arrays and complementary '-omics'. 相似文献