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131.
William Slikker George W. Lipe Glenn D. Newport 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1981,224(2):205-219
A method was developed to resolve radiolabeled estradiol-17β and its various metabolites in biological fluids and tissues. After a rapid initial clean-up step, samples were analyzed with the sequential use of reversed-phase and normal-phase high-performance liquid chromatographic systems. Approximately 25 conjugated and non-conjugated standards could be resolved by the combined use of six systems. Radiolabeled parent compound and metabolites from biological samples were separated and tentatively identified by comparing their retention times to those of known standards. The method was found to be reproducible and quantitative for the majority of the estrogens and their conjugates, and semiquantitative for some of the more polar and di-conjugated estrogens. 相似文献
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Hydrogels that mimic the natural extracellular matrix (ECM) are used in three-dimensional cell culture, cell therapy, and tissue engineering. A semi-synthetic ECM based on cross-linked hyaluronana offers experimental control of both composition and gel stiffness. The mechanical properties of the ECM in part determine the ultimate cell phenotype. We now describe a rheological study of synthetic ECM hydrogels with storage shear moduli that span three orders of magnitude, from 11 to 3 500 Pa, a range important for engineering of soft tissues. The concentration of the chemically modified HA and the cross-linking density were the main determinants of gel stiffness. Increase in the ratio of thiol-modified gelatin reduced gel stiffness by diluting the effective concentration of the HA component. 相似文献