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151.
Radomirka Nikolić Nevena Mitić Slavica Ninković Branka Vinterhalter Snežana Zdravković-Korać Mirjana Nešković 《Plant Growth Regulation》2010,62(2):181-188
Shoots of Lotus
corniculatus L., previously transformed with Agrobacterium
tumefaciens LBA4404/pTOK233, were grown in gibberellic acid (GA3)-containing media in an attempt to improve their growth and multiplication. Nodal stem segments of four poorly multiplying
clones, a clone with very good multiplication, and two non-transformed clones were incubated in media containing GA3 for 3 weeks, and then returned to a hormone-free medium for a further 3–5 subculture periods. Gibberellic acid increased
the number of axillary buds from one in controls to 2–13 shoots during the first 3 weeks of subculture in GA3-containing media. The multiplied buds, which apparently derived from additional axillaries, continued to multiply in hormone-free
medium, producing bunches of more than 50 shoots after 9 weeks. In addition, many nodal segments also produced callus tissues
at the basal end, or along the internode below the node, in which abundant shoot meristems formed de novo. Histological examination confirmed their origin via organogenesis. About 25% of regenerated shoots rooted spontaneously.
The application of auxins significantly improved rooting in more than 75% of all clones. Well-developed plantlets were produced
upon the transfer of rooted shoots to pots. No differences in responses to hormones were observed between LBA4404/pTOK233-transformed
clones and non-transformed clones, indicating that the reaction to GA3 was not the consequence of transformation events. The results point to a possible involvement of GA3 in lateral branching. They might also be recommended as a procedure suitable for increasing the production of transformed
plants in L. corniculatus. 相似文献
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Predicting the Open‐Circuit Voltage of CH3NH3PbI3 Perovskite Solar Cells Using Electroluminescence and Photovoltaic Quantum Efficiency Spectra: the Role of Radiative and Non‐Radiative Recombination 下载免费PDF全文
156.
Stefanović Milomir Djan Mihajla Veličković Nevena Demirbaş Yasin Paule Ladislav Gedeon Csongor István Posautz Annika Beiglböck Christoph Kübber-Heiss Anna Suchentrunk Franz 《Molecular biology reports》2020,47(4):2975-2984
Molecular Biology Reports - Toll-like receptors (TLRs) are transmembrane proteins of the innate immune system, composed of the ectodomain involved in pathogen recognition and the intracellular... 相似文献
157.
Nataša Ž. Šekuljica Jelena R. Jovanović Sonja M. Jakovetić Tanasković Nevena D. Ognjanović Ivana V. Gazikalović Zorica D. Knežević-Jugović Dušan Ž. Mijin 《Biotechnology progress》2020,36(4):e2991
Horseradish peroxidase (HRP) is a highly specific enzyme with great potential for use in the decolorization of synthetic dyes. A comprehensive study of HRP immobilization using various techniques such as adsorption and covalent immobilization on the novel carrier Purolite® A109 with a special focus on enzymatic decolorization and toxicity of artificially colored wastewater. The immobilized preparations with an activity of 156.21 ± 1.41 U g−1 and 85.71 ± 1.62 U g−1 after the HRP adsorption and covalent immobilization, respectively, were obtained. Stability and reusability of the immobilized preparations were also evaluated. A noteworthy decolorization level (~90%) with immobilized HRP was achieved. Phytotoxicity testing using Mung bean seeds and acute toxicity assay with Artemia salina has confirmed the applicability of the obtained immobilized preparation in industrial wastewater plants for the treatment of colored wastewater. 相似文献
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Ljiljana Tubić Goran Anačkov Jelena Milojević Nabil Ghalawenji Nevena Mitić Ružica Igić Snežana Zdravković-Korać 《Plant Cell, Tissue and Organ Culture》2014,118(1):101-110
This study assessed the shoot regeneration capacity of root-tips isolated from single seed-derived individual plants, obtained from a wild, open-pollinated micropopulation of shallot (Allium ascalonicum). Considerable variation was observed in the regeneration capacities of individual lines, ranging from 0.93 to 100 %, and a mean bud number per root explant between 0.09 and 20.67. One line was found to be superior, and was chosen for protocol optimization, focusing on the 2,4-D/BA ratio, duration of the CI phase and light conditions. The application of the optimized protocol to other lines, selected for their variable regeneration capacities, enhanced the process of regeneration and shortened the time required to obtain healthy plantlets, even in inferior lines. However, highly responsive lines remained superior, indicating that differences at the individual level must not be overlooked. The conditions employed in this study may serve as a base to facilitate the application of molecular breeding methods in shallot. 相似文献