全文获取类型
收费全文 | 2387篇 |
免费 | 226篇 |
出版年
2023年 | 21篇 |
2022年 | 23篇 |
2021年 | 88篇 |
2020年 | 48篇 |
2019年 | 59篇 |
2018年 | 68篇 |
2017年 | 53篇 |
2016年 | 94篇 |
2015年 | 182篇 |
2014年 | 179篇 |
2013年 | 181篇 |
2012年 | 232篇 |
2011年 | 208篇 |
2010年 | 136篇 |
2009年 | 105篇 |
2008年 | 146篇 |
2007年 | 130篇 |
2006年 | 107篇 |
2005年 | 103篇 |
2004年 | 103篇 |
2003年 | 82篇 |
2002年 | 81篇 |
2001年 | 12篇 |
2000年 | 13篇 |
1999年 | 10篇 |
1998年 | 10篇 |
1997年 | 15篇 |
1996年 | 9篇 |
1995年 | 9篇 |
1994年 | 6篇 |
1993年 | 8篇 |
1992年 | 7篇 |
1991年 | 4篇 |
1990年 | 5篇 |
1989年 | 4篇 |
1984年 | 3篇 |
1983年 | 7篇 |
1982年 | 5篇 |
1981年 | 4篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1978年 | 5篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1972年 | 2篇 |
1971年 | 6篇 |
1969年 | 4篇 |
1948年 | 2篇 |
1941年 | 2篇 |
1915年 | 2篇 |
排序方式: 共有2613条查询结果,搜索用时 31 毫秒
101.
de Souza N 《Nature methods》2007,4(2):116
Peptide counting in mass spectrometry allows researchers to draw a quantitative proteomic map of the ER and Golgi. 相似文献
102.
Dong A Xu X Edwards AM;Midwest Center for Structural Genomics;Structural Genomics Consortium Chang C Chruszcz M Cuff M Cymborowski M Di Leo R Egorova O Evdokimova E Filippova E Gu J Guthrie J Ignatchenko A Joachimiak A Klostermann N Kim Y Korniyenko Y Minor W Que Q Savchenko A Skarina T Tan K Yakunin A Yee A Yim V Zhang R Zheng H Akutsu M Arrowsmith C Avvakumov GV Bochkarev A Dahlgren LG Dhe-Paganon S Dimov S Dombrovski L Finerty P Flodin S Flores A Gräslund S Hammerström M Herman MD Hong BS 《Nature methods》2007,4(12):1019-1021
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain. 相似文献
103.
Natalie Krahn Markus Meier Raphael Reuten Manuel Koch Joerg Stetefeld Trushar R. Patel 《Biophysical journal》2019,116(11):2121-2130
UNCoordinated-6 (UNC-6) was the first member of the netrin family to be discovered in Caenorhabditis elegans. With homology to human netrin-1, it is a key signaling molecule involved in directing axon migration in nematodes. Similar to netrin-1, UNC-6 interacts with multiple receptors (UNC-5 and UNC-40, specifically) to guide axon migration in development. As a result of the distinct evolutionary path of UNC-6 compared to vertebrate netrins, we decided to employ an integrated approach to study its solution behavior and compare it to the high-resolution structure we previously published on vertebrate netrins. Dynamic light scattering and analytical ultracentrifugation on UNC-6 (with and without its C-domain) solubilized in a low-ionic strength buffer suggested that UNC-6 forms high-order oligomers. An increase in the buffer ionic strength resulted in a more homogeneous preparation of UNC-6, that was used for subsequent solution x-ray scattering experiments. Our biophysical analysis of UNC-6 ΔC solubilized in a high-ionic strength buffer suggested that it maintains a similar head-to-stalk arrangement as netrins ?1 and ?4. This phenomenon is thought to play a role in the signaling behavior of UNC-6 and its ability to move throughout the extracellular matrix. 相似文献
104.
Stilwell Justin M. Stilwell Natalie K. Camus Alvin C. Griffin Matt J. Rosser Thomas G. 《Systematic parasitology》2019,96(9):767-776
Systematic Parasitology - A Henneguya sp., morphologically resembling Henneguya nyongensis Fomena & Bouix, 1996, was isolated from the gills of Peter’s elephantnose fish, Gnathonemus... 相似文献
105.
106.
Natalie K. Goto Kevin H. Gardner Geoffrey A. Mueller Randall C. Willis Lewis E. Kay 《Journal of biomolecular NMR》1999,13(4):369-374
A selective protonation strategy is described that uses [3-2H] 13C -ketoisovalerate to introduce (1H- methyl)-leucine and (1H- methyl)-valine into 15N-, 13C-, 2H-labeled proteins. A minimum level of 90% incorporation of label into both leucine and valine methyl groups is obtained by inclusion of 100 mg/L -ketoisovalerate in the bacterial growth medium. Addition of [3,3-2H2] -ketobutyrate to the expression media (D2O solvent) results in the production of proteins with (1H-1 methyl)-isoleucine (>90% incorporation). 1H-13C HSQC correlation spectroscopy establishes that CH2D and CHD2 isotopomers are not produced with this method. This approach offers enhanced labeling of Leu methyl groups over previous methods that utilize Val as the labeling agent and is more cost effective. 相似文献
107.
108.
109.
Applying proteomics to signaling networks 总被引:3,自引:0,他引:3
The information from genome sequencing provides a new framework for a systems-wide understanding of protein networks and cellular function. Whereas microarray technologies provide information about global gene expression within cells, complementary proteomic strategies monitor expression of proteins and their posttranslational modifications. Improved technologies that have emerged for comprehensive and high-throughput protein analysis yield novel insights into cell regulation. 相似文献
110.
Walters JR Barley NF Khanji M Rhodes-Kendler O 《The Journal of steroid biochemistry and molecular biology》2004,(1-5):317-319
Intestinal absorption of dietary calcium is regulated by 1,25-dihydroxycholecalciferol (1,25(OH)(2)D(3)) in humans and in experimental animals but interspecies differences in responsiveness to 1,25(OH)(2)D(3) are found, possibly due to differences in the promoters of genes for intestinal calcium transport proteins or of the Vitamin D receptor (VDR). The epithelial calcium transporter, known as ECAC2 or CAT1, the product of the TRPV6 gene expressed in proximal intestinal enterocytes, is the first step in calcium absorption and studies in mice have shown that its expression is Vitamin D-dependent. In contrast in man, we showed that duodenal TRPV6 mRNA expression was independent of blood 1,25(OH)(2)D(3), although in Caco-2 cells, 1,25(OH)(2)D(3)-dependent changes have been demonstrated. We sought to explain these findings. A consensus Vitamin D response element in the mouse gene is absent in the human gene. We re-analysed our duodenal expression data according to a CDX2-site polymorphism in the VDR promoter. Mean TRPV6 expression was the same, but there was evidence of different responsiveness to 1,25(OH)(2)D(3). In the GG genotype group, but not the AG, duodenal TRPV6 expression increased with 1,25(OH)(2)D(3). We postulate that lower levels of expression of VDR in the GG group produce greater sensitivity to 1,25(OH)(2)D(3). 相似文献