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141.
Koji Kato Shigeji Jingu Naoyoshi Ogawa Shohei Higuchi 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2000,740(2):25-193
A liquid chromatographic–tandem mass spectrometric method for the rapid quantitative determination of pibutidine, an H2-receptor antagonist, in human urine has been developed and validated over the concentration range 0.1–25.6 μg ml−1. Urine samples were prepared based on a simple dilution with 0.05% acetic acid, followed by reversed-phase liquid chromatographic separation. Pibutidine and its internal standard (2H10-pibutidine) were ionized using an electrospray ionization interface and detected by tandem mass spectrometry in the selected reaction-monitoring mode. Completed validation demonstrated the method to be robust, accurate, precise and specific for the direct quantification of pibutidine in human urine. This method has enabled investigation of the urinary excretion of pibutidine following oral administration of pibutidine hydrochloride to healthy subjects. 相似文献
142.
Determinant in Human Immunodeficiency Virus Type 1 for Efficient Replication under Cytokine-Induced CD4+ T-Helper 1 (Th1)- and Th2-Type Conditions 下载免费PDF全文
Youichi Suzuki Yoshio Koyanagi Yuetsu Tanaka Tsutomu Murakami Naoko Misawa Naoyoshi Maeda Tohru Kimura Hisatoshi Shida James A. Hoxie William A. OBrien Naoki Yamamoto 《Journal of virology》1999,73(1):316-324
Cytokines are potent stimuli for CD4+-T-cell differentiation. Among them, interleukin-12 (IL-12) and IL-4 induce naive CD4+ T cells to become T-helper 1 (Th1) or Th2 cells, respectively. In this study we found that macrophage-tropic human immunodeficiency virus type 1 (HIV-1) strains replicated more efficiently in IL-12-induced Th1-type cultures derived from normal CD4+ T cells than did T-cell-line-tropic (T-tropic) strains. In contrast, T-tropic strains preferentially infected IL-4-induced Th2-type cultures derived from the same donor CD4+ T cells. Additional studies using chimeric viruses demonstrated that the V3 region of HIV-1 gp120 was the principal determinant for efficiency of replication. Cell fusion analysis showed that cells expressing envelope protein from a T-tropic strain effectively fused with IL-4-induced Th2-type culture cells. Flow cytometric analysis showed that the level of CCR5 expression was higher on IL-12-induced Th1-type culture cells, whereas CXCR4 was highly expressed on IL-4-induced Th2-type culture cells, although a low level of CXCR4 expression was observed on IL-12-induced Th1-type culture cells. These results indicate that HIV-1 isolates exhibit differences in the ability to infect CD4+-T-cell subsets such as Th1 or Th2 cells and that this difference may partly correlate with the expression of particular chemokine receptors on these cells. The findings suggest that immunological conditions are one of the factors responsible for inducing selection of HIV-1 strains. 相似文献
143.
M. Nishibori Naoki Nakaya Aiji Ohtsuka Takuro Murakami Kiyomi Saeki 《Histochemistry and cell biology》1998,110(1):51-56
B-43, a serine proteinase inhibitor belonging to the ovalbumin branch of the serpin superfamily, was purified and cloned
from bovine brain. Since [35S]-labeled B-43 forms SDS-stable complexes with pancreatic serine proteinases, trypsin, α-chymotrypsin, and kallikrein, it
has been suggested that B-43 is capable of inhibiting these serine proteinases and that B-43 may be present in the pancreas.
In the present study, we investigated the localization of B-43 in the bovine pancreas immunohistochemically and examined the
effect of B-43 on the amidolytic activities of pancreatic serine proteinases. Strong B-43-like immunoreactivity was localized
in acinar cells, especially in the basal sides of the cells where the rough endoplasmic reticulum is located. The nuclei of
the subpopulation of acinar cells were also immunoreactive for B-43. The recombinant glutathione S-transferase–B-43 fusion protein inhibited the amidolytic activity of trypsin and, to a lesser extent, α-chymotrypsin and
kallikrein, but not elastase. These results suggest a role of B-43 in regulating serine proteinases both in the cytoplasm
and the nucleus.
Accepted: 13 January 1998 相似文献
144.
Jinlian Chen Yoshihiro Inoue Naoyoshi Kumakura Kazuyuki Mise Ken Shirasu Yoshitaka Takano 《Molecular Plant Pathology》2021,22(8):1006-1013
Colletotrichum orbiculare infects cucurbits, such as cucumber and melon (Cucumis melo), as well as the model Solanaceae plant Nicotiana benthamiana, by secreting an arsenal of effectors that suppress the immunity of these distinct plants. Two conserved effectors of C. orbiculare, called NLP1 and NIS1, induce cell death responses in N. benthamiana, but it is unclear whether they exhibit the same activity in Cucurbitaceae plants. In this study, we established a new Agrobacterium-mediated transient expression system to investigate the cell death-inducing activity of NLP1 and NIS1 in melon. NLP1 strongly induced cell death in melon but, in contrast to the effects seen in N. benthamiana, mutations either in the heptapeptide motif or in the putative glycosylinositol phosphorylceramide-binding site did not cancel its cell death-inducing activity in melon. Furthermore, NLP1 lacking the signal peptide caused cell death in melon but not in N. benthamiana. Study of the transient expression of NIS1 also revealed that, unlike in N. benthamiana, NIS1 did not induce cell death in melon. In contrast, NIS1 suppressed flg22-induced reactive oxygen species generation in melon, as seen in N. benthamiana. These findings indicate distinct cell death-inducing activities of NLP1 and NIS1 in these two plant species that C. orbiculare infects. 相似文献
145.
Naoyoshi Chino Shigeru Kubo Mikiya Miyazato Masamitsu Nakazato Kenji Kangawa Shumpei Sakakibara 《Letters in Peptide Science》1996,3(1):45-52
Summary In a two-step selective disulfide-bond-forming reaction of human uroguanylin, a 16-residue peptide with two intramolecular disulfide bonds, two compounds (I and II) were formed, which could be detected by RP-HPLC after the second disulfide-bond-forming reaction and were isolated as single entities. Their primary structures, molecular weights, and disulfide connectivities proved to be identical, but their optical rotation values were different, suggesting that they are topological isomers. Only compound I was found to increase the cGMP levels in cultured T84 cells significantly. The ratio of these compounds was affected by the order of the disulfide-bond-forming reactions, but not by the solvent used. The presence of a carboxyl-terminal leucine residue seems to be crucial for stabilizing the conformation of the two isomers. 相似文献
146.
Naoyoshi Nishibori Masaru Niitsu Shinsuke Fujihara Takefumi Sagara Sachio Nishio & Ichiro Imai 《FEMS microbiology letters》2009,298(1):74-78
The polyamines caldopentamine and homocaldopentamine were detected in axenic strains of Chattonella antiqua and Heterosigma akashiwo ( Raphidophyceae ), respectively, as well as spermidine, the most abundant polyamine in both phytoplankton species. Trace amounts of putrescine, diaminopropane and norspermine were also detected in both species. Spermine was detected only from C. antiqua . These long linear polyamines are characteristic components of thermophilic bacteria. The detection from two species of Raphidophyceae indicates that the occurrence of long linear polyamines is not restricted to thermophilic microorganisms. 相似文献
147.
Tsuzuki Masayuki Motomura Kazuki Kumakura Naoyoshi Takeda Atsushi 《Journal of plant research》2017,130(2):211-226
Journal of Plant Research - Accumulation of an mRNA species is determined by the balance between the synthesis and the degradation of the mRNA. Individual mRNA molecules are selectively and... 相似文献
148.
Pamela Gan Ryoko Hiroyama Ayako Tsushima Sachiko Masuda Arisa Shibata Akiko Ueno Naoyoshi Kumakura Mari Narusaka Trinh Xuan Hoat Yoshihiro Narusaka Yoshitaka Takano Ken Shirasu 《Environmental microbiology》2021,23(10):6004-6018
Members of the Colletotrichum gloeosporioides species complex are causal agents of anthracnose in many commercially important plants. Closely related strains have different levels of pathogenicity on hosts despite their close phylogenetic relationship. To gain insight into the genetics underlying these differences, we generated and annotated whole-genome assemblies of multiple isolates of C. fructicola (Cf) and C. siamense (Cs), as well as three previously unsequenced species, C. aenigma (Ca), C. tropicale and C. viniferum with different pathogenicity on strawberry. Based on comparative genomics, we identified accessory regions with a high degree of conservation in strawberry-pathogenic Cf, Cs and Ca strains. These regions encode homologs of pathogenicity-related genes known as effectors, organized in syntenic gene clusters, with copy number variations in different strains of Cf, Cs and Ca. Analysis of highly contiguous assemblies of Cf, Cs and Ca revealed the association of related accessory effector gene clusters with telomeres and repeat-rich chromosomes and provided evidence of exchange between these two genomic compartments. In addition, expression analysis indicated that orthologues in syntenic gene clusters showed a tendency for correlated gene expression during infection. These data provide insight into mechanisms by which Colletotrichum genomes evolve, acquire and organize effectors. 相似文献
149.
Kenji Kangawa Naoto Minamino Naoyoshi Chino Shumpei Sakakibara Hisayuki Matsuo 《Biochemical and biophysical research communications》1981,99(3):871-878
After re-purification by reverse phase high performance liquid chromatography, α-neo-endorphin was submitted to structural analyses, performed by dansyl-Edman degradation, as well as by C-terminal analysis by 3H-labeling. The full sequence of α-neo-endorphin has been determined to be : Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro-Lys, in which the 8th residue previously reported as Arg is found to be Tyr. A synthetic decapeptide with the above sequence was verified to be identical with natural α-neo-endorphin. For further structural confirmation, tryptic and chymotryptic peptides were also identified. Thus, the complete sequence of α-neo-endorphin has been definitely established. Its potent opioid activity in the guinea-pig ileum assay is also discussed. 相似文献
150.
Nahoko Nishibori Toshi Shimamoto Naoto Nakamura Mari Shimokawa Hiroyuki Horiuchi Shuichi Furusawa Haruo Matsuda 《Biologicals》2004,32(4):213-218
The chicken is a useful animal for preparation of antibodies that are reactive with highly conserved mammalian molecules. For further clinical application of chicken antibodies, we constructed the novel expression vectors for chicken–human chimeric antibodies, pcSLCγ1, pcSLCγ4 and pcSLCκ. These vectors had the following characteristics: (1) any chicken variable regions from hybridomas or a phage display library can be easily introduced; (2) the variable regions are able to be expressed in different immunoglobulin isotypes; and (3) the chimeric antibodies can be highly expressed in either transiently or stably transfected eukaryotic cells (COS-7 and CHO-K1 cells). Western blot analysis of the chimeric antibodies revealed that the expressed products were of the predicted size, structure and specificity. These results indicate that these vectors are useful tools for the chimerization of chicken antibodies. 相似文献