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81.
Restriction of glucosyl-free HMC-DNA mediated by RglB is alleviated inrecBC sbcA strains ofEscherichia coli K12. Mutation in the unlinkedrra gene reverses thisrecBC sbcA-mediated alleviation. The map position ofrra is 90.16 min on the standard map, and therra
+ gene product counteracts Rgl restriction. The activation of therra gene is controlled by thesbcA gene, and this regulation does not seem to require the involvement of other gene functions. 相似文献
82.
83.
S. Easwaramoorthy H. David G. Santhalakshmi M. Shanmugasundaram V. Nandagopal N. K. Kurup 《BioControl》1990,35(3):385-391
Susceptibility of adults and puparia ofSturmiopsis inferens Tns. [Tachinidae, Diptera] to 9 commonly recommended insecticide sprays against sugarcane pests was determined. The chemicals tested as emulsifiable
concentrates include lindane 0.1%, endosulfan 0.1%, monocrotophos 0.05%, quinalphos 0.05%, malathion 0.1%, dimethoate 0.1%,
cypermethrin 0.01%, fenvalerate 0.01% and decamethrin 0.0014%. Lindane, malathion, dimethoate monocrotophos and quinalphos
were highly toxic, while decamethrin had little harmful effect to the adults when exposed for 6 h to filter paper impregnated
or sugarcane shoot bits sprayed with the chemicals. However, the insecticides had no harmful effect on the puparia and adult
emergence was normal from the puparia sprayed with insecticides.
In another study, susceptibility of adults to soil application of lindane EC, carbofuran G, chlorpyriphos G, Sevidol G and
whorl application of lindane G, chlorpyriphos G and Sevidol G was tested in pot culture. Except for soil application of lindane
EC, all other chemicals had no harmful effect to the adults in pot culture experiment. In a field trial, commonly recommended
insecticides against shoot borer,Chilo infuscatellus Snell.viz., soil application of granules of lindane, carbofuran, chlorpyriphos and Sevidol and folia spray of endosulfan did not affect
the parasite activity.
Institute Publication No 1030. 相似文献
84.
Molecular cloning and sequencing of mcrA locus and identification of McrA protein inEscherichia coli
R. Ramalingam Ranjan Prasad R. Shivapriya K. Dharmalingam 《Journal of biosciences》1992,17(3):217-232
The Mcr systems (previously known as Rgl systems) ofEscherichia coli recognize and cleave specific sequences carrying methylated or hydroxymethylated cytosines. We have cloned the mcrA gene
and determined its nucleotide sequence. An 831 base pair sequence encodes the McrA protein. Analysis of the sequence data
reveals that there arc additional ORFs internal to the above. A phage T7 expression system was used to determine the protein
products encoded by the cloned mcrA gene. The results clearly show that a 31 kDa polypeptide is responsible for McrA activity.
This is in agreement with the molecular weight deduced from sequence data. McrA protein was found to be localized in the outer
membrane ofEscherichia coli. To our knowledge this is the first restriction enzyme localized in the outer membraneof Escherichia coli.
Presented in part at the Second New England Biolabs Workshop on Biological DNA Modification, September, 1990, Berlin 相似文献
85.
R. Mathumathi S. Kumaravel K. Dharmalingam 《Applied microbiology and biotechnology》1990,33(3):291-295
Summary
Streptomyces lividans arginine auxotrophs which show amplification of a 5.7-kb DNA sequence, arose at a very high frequency, varying from 10% to 25% of Cmls spores. The amplifiable DNA sequence was shown to be stable over many generations. However, treatment of Cmls
arg mutants with subinhibitory concentrations of antibiotics such as spectinomycin, streptomycin, chloramphenicol, thiostrepton and kanamycin, either during sporulation or during vegetative growth of mycelia, led to the deletion of the entire amplified DNA sequence, including the left and right junction sequences. Depending upon the method of antibiotic treatment a reduction in the copy number of the amplified DNA was also observed. This reduction in copy number apparently occurred without drastically affecting the basic structure of the amplifiable unit of DNA. This phenomenon appears to be universal since deamplification and deletion were observed also in S. fradiae. Further, spontaneous arg mutants arose at much lower frequency from spectinomycin-pretreated Cmls cells compared to untreated cells. These arg mutants isolated in the presence of spectinomycin did not show amplification of the 5.7-kb sequence. Southern blot analysis using the 5.7-kb probe showed that the entire DNA sequence homologous to the amplifiable DNA sequence had been deleted.
Offprint requests to: K. Dharmalingam 相似文献