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Yu. V. Korzhov E. D. Lapshina D. I. Khoroshev A. V. Zakharchenko M. G. Kul’kov D. M. Yarkov 《Contemporary Problems of Ecology》2010,3(3):292-298
The results are given of the two-year study of the new technology of soil remediation from oil contamination-CLEANSOIL combining physicochemical and biological methods of eliminating oil pollution. It is shown that using this combined technology results in quick decrease in the oil concentration in soil to the level when the soil is able to clean itself. 相似文献
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Dlx genes, p63, and ectodermal dysplasias 总被引:4,自引:0,他引:4
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Gain-of-function and loss-of-function phenotypes of the protein phosphatase 2C HAB1 reveal its role as a negative regulator of abscisic acid signalling 总被引:13,自引:0,他引:13
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Zavodnik LB Zavodnik IB Lapshina EA Shkodich AP Bryszewska M Buko VU 《Biochemistry. Biokhimii?a》2000,65(8):946-951
Erythrocyte damage induced by hypochlorous acid (HOCl) results in cell lysis developing with time after the oxidant is removed (post-hemolysis). The apparent rate constant of post-hemolysis depends on time of incubation in the presence of HOCl and concentration of this oxidant. HOCl-dependent damage of erythrocyte membranes is associated with uncompetitive inhibition of the membrane-bound acetylcholinesterase. Genistein-8-C-glucoside is an isoflavonoid isolated from the flowers of Lupinus luteus L.; in aqueous solution, genistein-8-C-glucoside (0.5-2 mM) efficiently inhibited HOCl-induced damage to erythrocytes similar to the known HOCl scavengers taurine and reduced glutathione. This bioflavonoid can protect the erythrocyte membrane (and to a lesser extent, intraerythrocytic components) by interacting with the reactive chlorine species including hypochlorous acid and membrane-bound chloroamines formed in the reaction of HOCl with erythrocyte membrane proteins. 相似文献
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An alternative protocol for freeze-substitution is described. Araldite/Epon embedding medium (20% in acetone) is first used as a stabilizer (as e.g., OsO(4)) and then as embedding medium. The major components of the Araldite/Epon resin formulation react with proteins and lipids and provide for an excellent preservation and reasonable visualisation of the ultrastructure. The ultrastructural appearance can be deliberately influenced with the standard freeze-substitution procedure [Van Harreveld, A., Crowell, J., 1964. Electron microscopy after rapid freezing on a metal surface and substitution fixation. Anat. Rec. 149, 381-386.] using OsO(4) as stabilizing agent by protocols which degrade cytoplasmic and membrane proteins. Epoxy stabilized and embedded samples may become an important tool to get information about the effects of different reagents and protocols used in freeze-substitution. We believe that an in-depth understanding of the procedures is required to correctly interpret images and to complement studies of dynamic processes by light microscopy with reliable, highly detailed ultrastructural information. The block face of epoxy stabilized samples after ultrathin sectioning is highly suited for the analysis of the ultrastructure by AFM. 相似文献
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Differential induction of antiviral effects against West Nile virus in primary mouse macrophages derived from flavivirus-susceptible and congenic resistant mice by alpha/beta interferon and poly(I-C) 下载免费PDF全文
A cell model of primary macrophages isolated from the peritoneal cavity of flavivirus-susceptible and congenic resistant mice has been used to study the extent and kinetics of antiviral effects against West Nile virus upon priming with alpha/beta interferon (IFN-alpha/beta) or poly(I-C) (pIC). The pattern of flavivirus resistance expressed after priming of cells in this model was in good agreement with the pattern of flavivirus resistance described in the brains of the corresponding mouse strains. While priming with either IFN-alpha/beta or pIC completely blocked flavivirus replication in macrophages from resistant mice, it only transiently reduced flavivirus replication in macrophages from susceptible mice. It was only the combined pretreatment with IFN-alpha/beta and pIC that elicited strong antiviral responses that completely prevented flavivirus replication in macrophages from susceptible mice. Primary macrophages isolated from the blood of healthy human donors expressed a similar need for double-stranded RNA (dsRNA) cofactor in developing efficient antiviral responses against West Nile virus. These findings reveal that the inefficient IFN-alpha/beta-induced antiviral effects against flaviviruses in cells from susceptible hosts could be successfully complemented by an external dsRNA factor leading to the complete eradication of the virus. This treatment appears to compensate for the lack of an inborn resistance mechanism in cells from the susceptible host. Furthermore, it may also provide useful clues for the prevention and treatment of flavivirus infections. 相似文献
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The fibrinolytic properties of blood and erythrocytes were studied before and after physical exercise in male volunteers.
Their fibrinolytic responses were of two distinct types. In type 1 response, fibrinolytic activities of blood and erythrocytes
increased; the plasminogen activator and active plasmin contents in erythrocytes also increased, whereas the profibrinolysin
content correspondingly decreased. In addition, physical exercise increased the erythrocyte adsorption properties for plasma
activators of fibrinolysis. Type 2 response was characterized by a decrease in the fibrinolytic activity of blood; neither
fibrinolytic activity nor adsorption properties of erythrocytes increased. The type of blood and erythrocyte response to muscular
activity was determined by the pre-exercise level of red blood cell fibrinolytic activity. It was low in type 1 response due
to a lesser content of plasmin activators and greater content of antiplasmin. In type 2 response, the initially high lytic
capacity is connected with a greater reserve of activators and lesser reserve of inhibitors of the fibrinolytic system. A
conclusion was made that individual differences in fibrinolytic responses to physical exercise were largely accounted for
by the properties of erythrocytes. 相似文献