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91.
A retained intron in the 3′‐UTR of Calm3 mRNA mediates its Staufen2‐ and activity‐dependent localization to neuronal dendrites 下载免费PDF全文
92.
Fernando Moya 《Theorie in den Biowissenschaften》2000,119(3-4):318-333
Summary For Aristotle, living entities are exemplars of substance being. This means that they show a unity of matter and form on the
one hand and of potency and act on the other, in contrast to the duality shown in these respects by accidental beings, exemplified
by artefacts. An animal, although composed of the same elements (arche) considered by presocratic philosophers, is defined as an individual unity, generated and maintained by an organisation which
relates its parts in a hierarchical and functional way. Crucial to his understanding of the living is the hierarchy in which
each part is defined by fitness to a function, as an instrument (organon), performing within the whole. The whole being is also an instrument (an organism) for a specific kind of life, which actualises
an internal and specific principle (psuche).
Both the regularity of appearance of each organism and its fitness to a specific function justify the introduction, in addition
to the study of necessary causes, of an additional way of analysis in terms of hypothetical necessities, or necessary conditions
for a goal to be attained. Fitness to a function and regularity of appearance make necessary the analysis not just of the
elementary components, but of another principle (eidos, form) which defines a structure directed to a goal. While for accidental beings matter can survive their destruction, the
corruption (pthora) of living entities causes the disruption of the entire unity of matter and form. Living entities, both as matter and form,
show therefore a temporal limitation in being generated and corrupted, although they persist as specific forms since they
generate offspring which regularly share their differential characteristics defined in their form.
After reviewing recent interpretations of Aristotle’s biological writings, I will suggest the usefulness of this conceptual
framework to analyse some problems approached by current developmental biology. 相似文献
93.
Samantha Baldwin Roopashree Revanna Meeghan Pither-Joyce Martin Shaw Kathryn Wright Susan Thomson Leire Moya Robyn Lee Richard Macknight John McCallum 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2014,127(3):535-547
Key message
We present the first evidence for a QTL conditioning an adaptive trait in bulb onion, and the first linkage and population genetics analyses of candidate genes involved in photoperiod and vernalization physiology.Abstract
Economic production of bulb onion (Allium cepa L.) requires adaptation to photoperiod and temperature such that a bulb is formed in the first year and a flowering umbel in the second. ‘Bolting’, or premature flowering before bulb maturation, is an undesirable trait strongly selected against by breeders during adaptation of germplasm. To identify genome regions associated with adaptive traits we conducted linkage mapping and population genetic analyses of candidate genes, and QTL analysis of bolting using a low-density linkage map. We performed tagged amplicon sequencing of ten candidate genes, including the FT-like gene family, in eight diverse populations to identify polymorphisms and seek evidence of differentiation. Low nucleotide diversity and negative estimates of Tajima’s D were observed for most genes, consistent with purifying selection. Significant population differentiation was observed only in AcFT2 and AcSOC1. Selective genotyping in a large ‘Nasik Red × CUDH2150’ F2 family revealed genome regions on chromosomes 1, 3 and 6 associated (LOD > 3) with bolting. Validation genotyping of two F2 families grown in two environments confirmed that a QTL on chromosome 1, which we designate AcBlt1, consistently conditions bolting susceptibility in this cross. The chromosome 3 region, which coincides with a functionally characterised acid invertase, was not associated with bolting in other environments, but showed significant association with bulb sucrose content in this and other mapping pedigrees. These putative QTL and candidate genes were placed on the onion map, enabling future comparative studies of adaptive traits. 相似文献94.
We tested different fitness components on a series of conspecific mtDNA haplotypes, detected by RFLPs in Drosophila subobscura. Additionally, haplotype VIII, endemic to the Canary Islands, was tested upon its own native nuclear DNA background and upon that of the rest of mtDNAs tested herein. We found that both nuclear and mitochondrial DNA can have a significant effect upon their hosts' fitness, and that negative selection is one of the mechanisms that can intervene in this species' mtDNA haplotype pattern. We discuss the importance of this mechanism in relation to genetic drift, in the form of periodic population bottlenecks, and how the latter can enhance the former. We also detected a significant positive effect of haplotype VIII upon fitness that could explain in part the dominance of this endemic haplotype on some of the Canary Islands, and a mitochondrial heterosis involving this haplotype when on a foreign nuclear DNA background. 相似文献
95.
Wang B Moya N Niessen S Hoover H Mihaylova MM Shaw RJ Yates JR Fischer WH Thomas JB Montminy M 《Cell》2011,145(4):596-606
Under fasting conditions, metazoans maintain energy balance by shifting from glucose to fat burning. In the fasted state, SIRT1 promotes catabolic gene expression by deacetylating the forkhead factor FOXO in response to stress and nutrient deprivation. The mechanisms by which hormonal signals regulate FOXO deacetylation remain unclear, however. We identified a hormone-dependent module, consisting of the Ser/Thr kinase SIK3 and the class IIa deacetylase HDAC4, which regulates FOXO activity in Drosophila. During feeding, HDAC4 is phosphorylated and sequestered in the cytoplasm by SIK3, whose activity is upregulated in response to insulin. SIK3 is inactivated during fasting, leading to the dephosphorylation and nuclear translocation of HDAC4 and to FOXO deacetylation. SIK3 mutant flies are starvation sensitive, reflecting FOXO-dependent increases in lipolysis that deplete triglyceride stores; reducing HDAC4 expression restored lipid accumulation. Our results reveal a hormone-regulated pathway that functions in parallel with the nutrient-sensing SIRT1 pathway to maintain energy balance. 相似文献
96.
Ana Durb��n Juan J. Abell��n Nuria Jim��nez-Hern��ndez Marta Ponce Julio Ponce Teresa Sala Giuseppe D��Auria Amparo Latorre Andr��s Moya 《Microbial ecology》2011,61(1):123-133
Gut microbiota is the most complex bacterial community in the human body and its study may give important clues to the etiology of different intestinal diseases. Most studies carried out so far have used fecal samples, assuming that these samples have a similar distribution to the communities present throughout the colon. The present study was designed to test this assumption by comparing samples from the rectal mucosa and feces of nine healthy volunteers by sequencing libraries of 16S rRNA genes. At the family taxonomic level, where rarefaction curves indicate that the observed number of taxa is close to the expected one, we observe under different statistical analyses that fecal and mucosal samples cluster separately. The same is found at the level of species considering phylogenetic information. Consequently, it cannot be stated that both samples from a given individual are of similar composition. We believe that the evidence in support of this statement is strong and that it would not change by increasing the number of individuals and/or performing massive sequencing. We do not expect clinicians to stop using feces for research, but we think it is important to caution them on their potential lack of representativeness with respect to the bacterial biofilm on the rectal mucosa. 相似文献
97.
Trevor W Ghylin Sarahi L Garcia Francisco Moya Ben O Oyserman Patrick Schwientek Katrina T Forest James Mutschler Jeffrey Dwulit-Smith Leong-Keat Chan Manuel Martinez-Garcia Alexander Sczyrba Ramunas Stepanauskas Hans-Peter Grossart Tanja Woyke Falk Warnecke Rex Malmstrom Stefan Bertilsson Katherine D McMahon 《The ISME journal》2014,8(12):2503-2516
Members of the acI lineage of Actinobacteria are the most abundant microorganisms in most freshwater lakes; however, our understanding of the keys to their success and their role in carbon and nutrient cycling in freshwater systems has been hampered by the lack of pure cultures and genomes. We obtained draft genome assemblies from 11 single cells representing three acI tribes (acI-A1, acI-A7, acI-B1) from four temperate lakes in the United States and Europe. Comparative analysis of acI SAGs and other available freshwater bacterial genomes showed that acI has more gene content directed toward carbohydrate acquisition as compared to Polynucleobacter and LD12 Alphaproteobacteria, which seem to specialize more on carboxylic acids. The acI genomes contain actinorhodopsin as well as some genes involved in anaplerotic carbon fixation indicating the capacity to supplement their known heterotrophic lifestyle. Genome-level differences between the acI-A and acI-B clades suggest specialization at the clade level for carbon substrate acquisition. Overall, the acI genomes appear to be highly streamlined versions of Actinobacteria that include some genes allowing it to take advantage of sunlight and N-rich organic compounds such as polyamines, di- and oligopeptides, branched-chain amino acids and cyanophycin. This work significantly expands the known metabolic potential of the cosmopolitan freshwater acI lineage and its ecological and genetic traits. 相似文献
98.
Nelly Vicente-Dólera Christelle Troadec Manuel Moya Mercedes del Río-Celestino Teresa Pomares-Viciana Abdelhafid Bendahmane Belén Picó Belén Román Pedro Gómez 《PloS one》2014,9(11)
Although the availability of genetic and genomic resources for Cucurbita pepo has increased significantly, functional genomic resources are still limited for this crop. In this direction, we have developed a high throughput reverse genetic tool: the first TILLING (Targeting Induced Local Lesions IN Genomes) resource for this species. Additionally, we have used this resource to demonstrate that the previous EMS mutant population we developed has the highest mutation density compared with other cucurbits mutant populations. The overall mutation density in this first C. pepo TILLING platform was estimated to be 1/133 Kb by screening five additional genes. In total, 58 mutations confirmed by sequencing were identified in the five targeted genes, thirteen of which were predicted to have an impact on the function of the protein. The genotype/phenotype correlation was studied in a peroxidase gene, revealing that the phenotype of seedling homozygous for one of the isolated mutant alleles was albino. These results indicate that the TILLING approach in this species was successful at providing new mutations and can address the major challenge of linking sequence information to biological function and also the identification of novel variation for crop breeding. 相似文献
99.
Diego Santos-Garcia Pierre-Antoine Farnier Francisco Beitia Einat Zchori-Fein Fabrice Vavre Laurence Mouton Andrés Moya Amparo Latorre Francisco J. Silva 《Journal of bacteriology》2012,194(23):6654-6655
The genome of “Candidatus Portiera aleyrodidarum,” the primary endosymbiont of the whitefly Bemisia tabaci (Mediterranean species), is reported. It presents a reduced genome (357 kb) encoding the capability to synthetize, or participate in the synthesis of, several amino acids and carotenoids, being the first insect endosymbiont capable of supplying carotenoids. 相似文献
100.