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991.
A. Minerva Arce-Ibarra Anthony Charles 《Human ecology: an interdisciplinary journal》2008,36(6):853-860
This study explores the management of inland fisheries on common lands (ejidos) within the Mayan Zone of Quintana Roo, Mexico. Qualitative survey data are used to examine whether there are (a) ongoing
fishery conservation activities; (b) local caretakers of fishing sites; (c) restrictions on where fishing can take place;
and (d) particular groups of individuals who lack the right to fish within the ejidos. Results indicate that in contrast to other local resources, fisheries in the ejidos have less defined use rights, being viewed as largely “open access”. In particular, most feel that outsiders can fish in
an ejido if they abide by local rules (notably not damaging the local water bodies, asking consent to fish, and having a friend or
relative in the community). However, there are within-community differences in views concerning management and conservation,
with those more dependent on fishing being more likely to support exclusion of outsiders. 相似文献
992.
Pucciarelli HM González-José R Neves WA Sardi ML Rozzi FR 《Journal of human evolution》2008,54(3):296-308
In a recent study we found that crania from South Amerindian populations on each side of the Andes differ significantly in terms of craniofacial shape. Western populations formed one morphological group, distributed continuously over 14,000km from the Fuegian archipelago (southern Chile) to the Zulia region (northwestern Venezuela). Easterners formed another group, distributed from the Atlantic Coast up to the eastern foothills of the Andes. This differentiation is further supported by several genetic studies, and indirectly by ecological and archaeological studies. Some authors suggest that this dual biological pattern is consistent with differential rates of gene flow and genetic drift operating on both sides of the Cordillera due to historical reasons. Here we show that such East-West patterning is also observable in North America. We suggest that the "ecological zones model" proposed by Dixon, explaining the spread of the early Americans along a Pacific dispersal corridor, combined with the evolution of different population dynamics in both regions, is the most parsimonious mechanism to explain the observed patterns of within- and between-group craniofacial variability. 相似文献
993.
Spatial Heterogeneity of Bacterial Populations in Monomictic Lake Estanya (Huesca,Spain) 总被引:2,自引:0,他引:2
Bacterial population changes were investigated in the monomictic Lake Estanya by combining microscopic analysis and two molecular
methods involving the amplification of 16S rDNA genes using primers for the domain Bacteria and subsequent restriction fragment length polymorphism (PCR–RFLP) and denaturing gradient gel electrophoresis (PCR–DGGE).
Both approaches revealed the vertical distribution of predominant microbial morphotypes and phylotypes in both holomictic
and stratified periods, respectively, and showed that variations in structure and composition of bacterial populations are
occurring in this lake as a function of depth and time. Through principal component analysis (PCA), these shifts could be
related to different physicochemical parameters with temperature, oxygen concentration, and the incident light being of paramount
importance as structuring variables. Comparison of RFLP and DGGE profiles by scoring similarities using the Jaccard coefficient
and then building a multidimensional scaling map (MDS) showed equivalent results. Both techniques revealed that bacterial
populations, present in the whole water column in the holomictic period, showed a high similarity with those located in the
deeper part of the lake in the stratified period, evidencing that other factors, both biotic and abiotic, should also be considered
as a force driving change in the composition of the bacterial community. Furthermore, DGGE analysis showed that sequences
from prominent bands were affiliated to members of four major phyla of the domain Bacteria: Cyanobacteria, Bacteroidetes, Proteobacteria, and Actinobacteria, most of which corresponded to heterotrophic bacterial populations involved in carbon, sulfide, and nitrogen biogeochemical
cycles, which were indistinguishable under the light microscope. 相似文献
994.
Levi V Villamil Giraldo AM Castello PR Rossi JP González Flecha FL 《The Biochemical journal》2008,416(1):145-152
Non-enzymatic glycation of biomolecules has been implicated in the pathophysiology of aging and diabetes. Among the potential targets for glycation are biological membranes, characterized by a complex organization of lipids and proteins interacting and forming domains of different size and stability. In the present study, we analyse the effects of glycation on the interactions between membrane proteins and lipids. The phospholipid affinity for the transmembrane surface of the PMCA (plasma-membrane Ca(2+)-ATPase) was determined after incubating the protein or the phospholipids with glucose. Results show that the affinity between PMCA and the surrounding phospholipids decreases significantly after phosphospholipid glycation, but remains unmodified after glycation of the protein. Furthermore, phosphatidylethanolamine glycation decreases by approximately 30% the stability of PMCA against thermal denaturation, suggesting that glycated aminophospholipids induce a structural rearrangement in the protein that makes it more sensitive to thermal unfolding. We also verified that lipid glycation decreases the affinity of lipids for two other membrane proteins, suggesting that this effect might be common to membrane proteins. Extending these results to the in vivo situation, we can hypothesize that, under hyperglycaemic conditions, glycation of membrane lipids may cause a significant change in the structure and stability of membrane proteins, which may affect the normal functioning of membranes and therefore of cells. 相似文献
995.
The head lice, Pediculus humanus capitis (Phthiraptera:Pediculidae), is an obligate ectoparasite of humans that causes pediculosis capitis, a nuisance for millions
of people worldwide, with high prevalence in children. Pediculosis capitis has been treated by methods that include the physical
remotion of lice, various domestic treatments and conventional insecticides. None of these methods render complete protection,
and there is clear evidence for the evolution of resistance and cross-resistance to conventional insecticides. Non-toxic alternative
options are hence needed for head lice treatment and/or prevention, and natural products from plants, especially essential
oils (EOs), are good candidates for safer control agents that may provide good anti-lice activity and low levels of evolved
resistance. A few EOs have been tested as repellents with promissory results, although often in vitro tests and clinical trials
produce contradictory results. A handful of fixed extracts and several EOs and their individual components have also been
tested as contact pediculicides or fumigants. The studies have focused mainly on plant families characterized for the production
of EOs. While many EOs and individual compounds showed pediculicide activity, comparing results is difficult due to the diverse
bioassay methodologies. Studies of anti-lice activity of individual EO components provide the basis for preliminary conclusions
of structure–activity relationships, although no clear patterns can yet be drawn. We here attempt to provide a concise compilation
of the available information on anti-lice activity of plant extracts and plant-derived compounds, which we hope may be of
help for future developments in this area.
相似文献
相似文献
996.
Breast cancer is a first magnitude problem of public health worldwide. There is increasing evidence that this cancer is originated in and maintained by a small population of undifferentiated cells with self-renewal properties. This small population generates a more differentiated pool of cells which represents the main mass of the tumor, resembling the hierarchical tissue organization of the normal breast. These cancer stem cells seem to share a similar phenotype with their normal counterparts but they display dysfunctional patterns of proliferation and differentiation, and they no longer respond to normal physiological controls that ensure a balanced cellular turnover. The origin of these cancer stem cells is controversial; it is not well known if they are originated from normal stem cells or from more differentiated progenitors where a de novo stem cell program is activated by the oncogenic insult. Here we review the origin of breast cancer stem cells and their role in the pathogenesis of cancer development, together with their implications in breast cancer progression, treatment and prognosis. 相似文献
997.
Yáñez JM González R Angulo J Vidal R Santos JL Martínez V 《Molecular ecology resources》2008,8(6):1442-1444
The emu (Dromaius novaehollandiae), a member of ratite family, is native to Australia and has been introduced to other countries worldwide. In this work, 10 polymorphic microsatellite loci were isolated and characterized for emu from public sequences. Polymorphism was surveyed in 22 individuals from two different populations kept in captivity. Between two and 11 alleles were found per locus, and the observed heterozygosity ranged from 0.05 to 0.85, in accordance with expectations. These markers will be useful as tools for detecting levels of genetic variation, reconstructing pedigrees (for quantitative genetic analysis) and identifying markers associated to fitness traits in emu populations. 相似文献
998.
Cuervo P Cupolillo E Britto C González LJ E Silva-Filho FC Lopes LC Domont GB De Jesus JB 《Journal of Proteomics》2008,71(1):109-122
A comparative analysis of proteomic maps of long-term grown and fresh clinical Trichomonas vaginalis isolates exhibiting low and high virulence phenotypes, respectively, was performed using two-dimensional gel electrophoresis and mass spectrometry. Of 29 protein spots differentially expressed between the isolates, 19 were over-expressed in the isolate exhibiting high virulence phenotype: proteins associated with cytoskeletal dynamics, such as coronin and several isoforms of actin, as well as proteins involved in signal transduction, protein turnover, proteolysis, and energetic and polyamine metabolisms were identified. Some malate dehydrogenase, fructose-1,6-bisphosphate aldolase and ornithine cyclodeamidase isoforms were exclusively expressed by the highly virulent isolate. During interaction assays with VEC, parasites exhibiting high virulence phenotype rapidly adhered and switched to amoeboid forms. In contrast, low adhesion and no morphological transformation were observed in parasites displaying low virulence phenotype. Our findings demonstrate that expression of specific proteins by high and low virulence parasites could be associated with the ability of each isolate to undergo morphological transformation and interact with host cells. Such data represent an important step towards understanding of the complex interaction network of proteins that participate in the mechanism of pathogenesis of this protozoan. 相似文献
999.
Gianotti TF Sookoian S Dieuzeide G García SI Gemma C González CD Pirola CJ 《Obesity (Silver Spring, Md.)》2008,16(7):1591-1595
The aim of this study was to investigate whether mitochondrial DNA (mtDNA) content is associated with insulin resistance (IR) in a sample of adolescents with features of metabolic syndrome. We further studied the link between polymorphisms in three genes involved in mitochondrial biogenesis and the presence of deleted mtDNA and mtDNA content. Data and blood samples were collected from 175 adolescents out of a cross-sectional, population-based study of 934 high school students. On the basis of the median value of homeostasis model assessment of IR (HOMA-IR) of the whole sample (2.2), the population was divided into two groups: noninsulin resistance (NIR) and IR. mtDNA quantification using nuclear DNA (nDNA) as a reference was carried out using a real-time quantitative PCR method. Genotyping for peroxisome proliferator-activated receptor-gamma (PPAR-gamma) (pro12Ala), PPAR- gamma coactivator-1alpha (PGC-1alpha) (Gly482Ser), and Tfam (rs1937 and rs12247015) polymorphisms was performed by PCR-based restriction fragment length polymorphism. Long-extension PCR was performed to amplify the whole mitochondrial genome. The mtDNA/nDNA ratio was significantly lower in the IR group (median: 9.08, range: 68.94) in comparison with the NIR group (12.24, 71.92) (P<0.03). Besides, the mtDNA/nDNA ratio was inversely correlated with HOMA (R: -0.18, P<0.02), glucose (R: -0.21, P<0.008), and uric acid (R: -0.18, P<0.03). Genotypes for the PPAR- gamma, PGC-1alpha, and Tfam variants were not associated with the mtDNA/nDNA ratio. Long-extension PCR did not show significant levels of mtDNA deletions. In conclusion, our findings indicate that reduced mtDNA content in peripheral leukocytes is associated with IR. This result seems not to be related with the previously mentioned variants in genes involved in the regulation of mitochondrial biogenesis. 相似文献
1000.
Sáez ME González-Sánchez JL Ramírez-Lorca R Martínez-Larrad MT Zabena C González A Morón FJ Ruiz A Serrano-Ríos M 《PloS one》2008,3(8):e2953
Cardiovascular disease is the leading cause of morbidity and mortality in the industrialized world. Familial aggregation of cardiovascular risk factors is a frequent finding, but genetic factors affecting its presentation are still poorly understood. The calpain 10 gene (CAPN10) has been associated with type 2 diabetes (T2DM), a complex metabolic disorder with increased risk of cardiovascular disease. Moreover, the CAPN10 gene has been associated with the presence of metabolic syndrome (MS) in T2DM and in polycystic ovary syndrome (PCOS). In this work, we have analysed whether the polymorphisms UCSNP44, -43, -19 and -63 are related to several cardiovascular risk factors in the context of MS. Molecular analysis of CAPN10 gene was performed in 899 individuals randomly chosen from a cross-sectional population-based epidemiological survey. We have found that CAPN10 gene in our population is mainly associated with two indicators of the presence of insulin resistance: glucose levels two hours after a 75-g oral glucose tolerance test (OGTT) and HOMA values, although cholesterol levels and blood pressure values are also influenced by CAPN10 variants. In addition, the 1221/1121 haplogenotype is under-represented in individuals that fulfil the International Diabetes Federation (IDF) diagnostic criteria for MS. Our results suggest that CAPN10 gene is associated with insulin resistance phenotypes in the Spanish population. 相似文献