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Senkiti Sakai Mihir R. Banerjee 《Biochimica et Biophysica Acta (BBA)/General Subjects》1979,582(1):79-88
Prolactin receptors were monitored by measuring 125I-labeled prolactin binding to collagenase-dissociated mammary epithelial cells of lactating BALB/c mice. Specific receptors for iodine-labeled prolactin with an apparent dissociation constant (Kd) of 0.99 · 10?9 M were present on the dissociated mammary cells. The binding was inhibited by ovine prolactin, human growth hormone and human placental lactogen but not by follicle stimulating hormone, luteinizing hormone, thyroid stimulating hormone, bovine growth hormone or insulin. Adrenal ablation of nursing mothers caused a reduction of the number of prolactin receptors and this effect was preventable by hydrocortisone therapy. Hydocortisone injections to mothers 3 days after adrenalectomy also induced a replenishment of the prolactin receptors on the mammary cells. Injections of progesterone failed to sustain the high level of mammary cell prolactin receptors in adrenalectomized animals. Stimultaneous injections of hydrocortisone and progesterone to animals 3 days after adrenalectomy caused a partial suppression of the stimulatory action of hydrocortisone alone. The results suggest that hydrocortisone can exert a modulatory influence on mammary cell prolactin receptors in non-hypophysectomized post-partum mice without altering the dissociation constant (Kd) of the receptors. 相似文献
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New fluorescent polymeric materials were synthesized by grafting the nucleobase cytosine on to the backbone of agarose and κ-carrageenan, employing a rapid water based method under microwave irradiation using potassium persulphate (KPS) as an initiator. The emission spectrum of the modified agarose and κ-carrageenan recorded in aqueous solution (5 × 10−5 M) exhibited emission maxima (λem,max) at 348 nm by excitation at 266 nm. The emission intensity was enhanced by ca. 104% and 60% compared to that of pure cytosine solution of the same concentration. When the concentration of the pure cytosine solution is made equivalent to the concentration of the cytosine molar component (3.09 × 10−5) and (3.5 × 10−5) present in 5 × 10−5 M solution of modified agarose and κ-carrageenan, respectively, then ca. 143% and 81% enhancement in emission intensity was observed. The remarkable fluorescent activity of the agarose-cytosine derivative may have potential uses as sensor in various applications. 相似文献
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Genetic Evidence for Preferential Strand Transfer during Meiotic Recombination in Yeast 总被引:7,自引:2,他引:5 下载免费PDF全文
During meiotic recombination in the yeast Saccharomyces cerevisiae, heteroduplexes are formed as an intermediate in the exchange process. In the formation of an asymmetric heteroduplex, one chromosome acts as a donor of a single DNA strand and the other acts as a recipient. We present genetic evidence that the nontranscribed strand is donated more frequently than the transcribed strand in spores that have an unrepaired mismatch at the HIS4 locus. 相似文献