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31.
The energy metabolism of Trypanosomatidae has been the subject of many reviews during the past decade. In recent years, however, new data have led to a more complete picture of trypanosomatid metabolism and a reappraisal of the role of some characteristic organelles in the energy supply of these parasites. For years, the glycosome was thought to be a peroxisome-like organelle that had evolved to allow the parasites to carry out glycolysis at a high rate using a relatively small amount of enzyme. However, the results of recent studies of trypanosomatid glycolysis and the detection of various other pathways and enzymes in the organelle necessitate a modification of this view. Here, Paul Michels, Véronique Hannaert and Frédéric Bringaud review the new data and discuss the possible implications for our view on the role of the glycosome. 相似文献
32.
Duffieux F Van Roy J Michels PA Opperdoes FR 《The Journal of biological chemistry》2000,275(36):27559-27565
Trypanosomatids are parasitic protists that have part of their glycolytic pathway sequestered inside peroxisome-like organelles: the glycosomes. So far, at least one enzyme of the pentose-phosphate pathway has been found to be associated partially with glycosomes. Here, we describe how two genes from Trypanosoma brucei, coding for the first two enzymes of the pentose-phosphate pathway, i.e. glucose-6-phosphate dehydrogenase and 6-phosphogluconolactonase, were identified by in silico screening of trypanosome genome project data bases. These genes were cloned and sequenced. Analysis of the lactonase sequence revealed that it contained a C-terminal peroxisome targeting signal in agreement with its subcellular localization in the bloodstream form trypanosome (15% glycosomal and 85% cytosolic). However, the dehydrogenase sequence did not reveal any targeting signal, despite its localization inside glycosomes. The corresponding enzymes have been overexpressed in Escherichia coli and purified, and their biochemical characteristics have been determined. 相似文献
33.
The REG1 gene encodes a regulatory subunit of the type-1 protein phosphatase (PP1) Glc7 in Saccharomyces cerevisiae, which directs the catalytic subunit to substrates involved in glucose repression. Loss of REG1 relieves glucose repression of many genes, including the MAL structural genes that encode the maltose fermentation enzymes. In this report, we explore the role of Reg1p and its homolog
Reg2p in glucose-induced inactivation of maltose permease. Glucose stimulates the proteolysis of maltose permease and very
rapid loss of maltose transport activity – more rapid than can be explained by loss of the permease protein alone. In a reg1Δ strain we observe a significantly reduced rate of glucose-induced proteolysis of maltose permease, and the rapid loss of
maltose transport activity does not occur. Instead, surprisingly, the slow rate of proteolysis of maltose permease is accompanied
by an increase in maltose transport activity. Loss of Reg2p modestly reduces the rates of both glucose-induced proteolysis
of maltose permease and inactivation of maltose transport activity. Overexpression of Reg2p in a reg1Δ strain suppresses the effect on maltose permease proteolysis and partially restores the inactivation of maltose transport
activity, but does not affect the insensitivity of MAL gene expression to repression by glucose observed in this strain. Thus, protein phosphatase type-1 (Glc7p-Reg1p and Glc7p-Reg2p)
plays a role in transduction of the glucose signal during glucose-induced proteolysis of maltose permease, but only Glc7p-Reg1p
is involved in glucose-induced inactivation of maltose transport activity and glucose repression of MAL gene expression. Overexpression of REG1 partially restores proteolysis of maltose permease in a grr1Δ strain, which lacks glucose signaling, but does not rescue rapid inactivation of maltose transport activity or sensitivity
to glucose repression. A model for the role of Reg1p and Reg2p in glucose signaling pathways is discussed. We also uncovered
a previously unrecognized G2/M delay in the grr1Δ but not the reg1Δ strains, and this delay is suppressed by REG1 overexpression. The G1/S delay seen in grr1Δ mutants is slightly suppressed as well, but REG1 overexpression does not suppress other grr1Δ phenotypes such as insensitivity to glucose repression.
Received: 21 October 1999 / Accepted: 28 December 1999 相似文献
34.
Comparison and Evolutionary Analysis of the Glycosomal Glyceraldehyde-3-Phosphate Dehydrogenase from Different Kinetoplastida 总被引:4,自引:0,他引:4
Véronique Hannaert Fred R. Opperdoes Paul A.M. Michels 《Journal of molecular evolution》1998,47(6):728-738
In this work, we present the sequences and a comparison of the glycosomal GAPDHs from a number of Kinetoplastida. The complete
gene sequences have been determined for some species (Crithidia fasciculata, Herpetomonas samuelpessoai, Leptomonas seymouri, and Phytomonas sp), whereas for other species (Trypanosoma brucei gambiense, Trypanosoma congolense, Trypanosoma vivax, and Leishmania major), only partial sequences have been obtained by PCR amplification. The structure of all available glycosomal GAPDH genes was
analyzed in detail. Considerable variations were observed in both their nucleotide composition and their codon usage. The
GC content varies between 64.4% in L. seymouri and 49.5% in the previously sequenced GAPDH gene from Trypanoplasma borreli. A highly biased codon usage was found in C. fasciculata, with only 34 triplets used, whereas in T. borreli 57 codons were employed. No obvious correlation could be observed between the codon usage and either the nucleotide composition
or the level of gene expression. The glycosomal GAPDH is a very well-conserved enzyme. The maximal overall difference observed
in the amino acid sequences is only 25%. Specific insertions and extensions are retained in all sequences. The residues involved
in catalysis, substrate, and inorganic phosphate binding are fully conserved, whereas some variability is observed in the
cofactor-binding pocket. The implications of these data for the design of new trypanocidal drugs targeted against GAPDH are
discussed. All available gene and amino acid sequences of glycosomal GAPDHs were used for a phylogenetic analysis. The division
of the Kinetoplastida into two suborders, Bodonina and Trypanosomatina, was well supported. Within the letter group, the Trypanosoma species appeared to be monophyletic, whereas the other trypanosomatids form a second clade.
Received: 23 February 1998/Accepted: 26 March 1998 相似文献
35.
Anne M. Michels 《Primates; journal of primatology》1998,39(4):549-556
Competition for food within the social group has been postulated as an important factor affecting primate social organization.
This study examined how factors such as sex, aggression, dispersion of food, and amount of difficulty involved in obtaining
food affect the distribution of food in common marmoset (Callithrix jacchus) social groups. Mated pairs of adultC. jacchus were presented with food that was either dispersed or concentrated, and either difficult or easy, to obtain. The number of
food pieces obtained, aggressive displays, incidents of physical aggression, and amount of time spent searching for food were
recorded for each animal. Neither dispersion nor difficulty in obtaining food had significant effects on the distribution
of food or any of the other behaviors examined. The primary factors affecting food distribution were aggression and amount
of time spent searching. Females obtained more food than males in all situations because they were more aggressive and apparently
more motivated to search for food than were males. 相似文献
36.
Martin Chopra Pascal Link Christine Michels Dieter Schrenk 《Cell biology and toxicology》2010,26(3):239-254
The main target organ of the mycotoxin ochratoxin A (OTA) in mammals is the kidney but OTA has also been shown to be hepatotoxic
in rats and to induce tumors in mouse liver. Even at very low concentrations, OTA causes perturbations of cellular signaling
pathways as well as enhanced apoptosis. OTA has been extensively studied in kidney cell systems. Since this substance also
affects liver health, we focused our work on apoptosis-related events induced by OTA in primary rat hepatocytes. We performed
pathway-specific polymerase chain reaction arrays to assess the expression of genes involved in apoptosis. Treatment with
1 μM OTA for 24 h caused marked changes in apoptosis-related gene expression. Genes as apaf1, bad, caspase 7, polb (DNA polymerase
beta, performs base excision repair), and p53, which are marker genes for DNA damage, were upregulated. FAS and faslg were
also markedly induced by treatment with OTA. Treatment of hepatocytes with OTA led to a concentration-dependent inhibition
of protein biosynthesis. Apoptosis-inducing factor was released from mitochondria following OTA treatment; the mycotoxin induced
the activity of caspases 8, 9, and 3/7 and caused chromatin condensation and fragmentation. Caspase inhibition led to a significant
but not complete reduction of OTA-induced apoptosis. Our data suggest that not only OTA leads to p53-dependent apoptosis in
rat hepatocytes but it also hints to other mechanisms, independent of caspase activation or protein biosynthesis, being involved. 相似文献
37.
Cordeiro AT Cáceres AJ Vertommen D Concepción JL Michels PA Versées W 《Journal of molecular biology》2007,372(5):1215-1226
Glucose is an essential substrate for Trypanosoma cruzi, the protozoan organism responsible for Chagas' disease. The glucose is intracellularly phosphorylated to glucose 6-phosphate. Previously, a hexokinase responsible for this phosphorylation has been characterized. Recently, we identified an ATP-dependent glucokinase in T. cruzi exhibiting a tenfold lower substrate affinity compared to the hexokinase. Both enzymes, which belong to very different groups of the same family, are located inside glycosomes, the peroxisome-like organelles of Kinetoplastida that are known to contain the first seven glycolytic steps as well as enzymes of the oxidative branch of the pentose phosphate pathway. Here, we present the crystallographic structure of T. cruzi glucokinase, in complex with glucose and ADP. The structure suggests a loose tetrameric assembly formed by the association of two tight dimers. TcGlcK was previously reported to exist in a concentration-dependent equilibrium of monomeric and dimeric states. Here, we used mass spectrometry analysis to confirm the existence of TcGlcK monomeric and dimeric states. The analysis of subunit interactions and comparison with the bacterial glucokinases give insights into the forces promoting the stability of the different oligomeric states. Each T. cruzi glucokinase monomer contains one glucose and one ADP molecule. In contrast to hexokinases, which show a moderate preference for the alpha anomer of glucose, the electron density clearly shows the d-glucose bound in the beta configuration in the T.cruzi glucokinase. Kinetic assays with alpha and beta-d-glucose further confirm a moderate preference of the T. cruzi glucokinase for the beta anomer. Structural comparison of the glucokinase and hexokinases permits the identification of a possible mechanism for anomer selectivity in these hexose-phosphorylating enzymes. The preference for distinct anomers suggests that in T. cruzi hexokinase and glucokinase are not directly competing for the same substrate and are probably both present because they exert distinct physiological functions. 相似文献
38.
Boudier C Bousquet JA Schauinger S Michels B Bieth JG 《Archives of biochemistry and biophysics》2007,466(2):155-163
The inhibitory activity of the serpins alpha(1)-proteinase inhibitor, alpha(1)-antichymotrypsin, alpha(2)-antiplasmin, antithrombin and C(1)-esterase inactivator is rapidly lost at pH 3 but slowly recovers at pH 7.4 with variable first-order rates (t(1/2)=1.4-19.2 min). All except alpha(1)-antichymotrypsin undergo a variation in intrinsic fluorescence intensity upon acidification (midpoint ca. 4.5) with a slow bi-exponential return to the initial intensity at pH 7.4 (mean t(1/2)=2.3-23 min). No correlation was found between the time of fluorescence recovery and that of reactivation. The acid-treated serpins are proteolyzed at neutral pH by their target proteinases. alpha(1)-Proteinase inhibitor was studied in more detail. Its acidification at pH 3 has a mild effect on its secondary structure, strongly disorders its tertiary structure, changes the microenvironment of Cys(232) and causes a very fast change in ellipticity at 225 nm (t(1/2)=1.6s). Neutralization of the acid-treated alpha(1)-proteinase inhibitor is an exothermic phenomenon. It leads to a much faster recovery of activity (t(1/2)=4+/-1 min) than of fluorescence intensity (t(1/2)=23+/-19 min), ellipticity (t(1/2)=32+/-4 min) and change in total energy, indicating that the inhibitory activity of alpha(1)-proteinase inhibitor does not require a fully native structure. 相似文献
39.
Inactivation of Influenza A Virus on Copper versus Stainless Steel Surfaces 总被引:2,自引:0,他引:2
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Influenza A virus particles (2 × 106) were inoculated onto copper or stainless steel and incubated at 22°C at 50 to 60% relative humidity. Infectivity of survivors was determined by utilizing a defined monolayer with fluorescent microscopy analysis. After incubation for 24 h on stainless steel, 500,000 virus particles were still infectious. After incubation for 6 h on copper, only 500 particles were active. 相似文献
40.
Helen Michels Susanne Lildal Amsinck Erik Jeppesen Luc De Meester 《Hydrobiologia》2007,594(1):117-129
In shallow temperate lakes, zooplankton populations may exhibit diel horizontal migration (DHM) and move towards macrophytes
during the day to avoid fish. Using a natural Daphnia magna population, we undertook an experimental investigation aimed to describe the genetic variation for DHM and to study whether
an adaptive micro-evolutionary response occurred to changes in macrophyte coverage and fish predation pressure through time.
Twenty-seven D. magna clones were hatched from ephippia in the sediment of shallow Lake Ring, Denmark. This lake was eutrophied during the 20th
century and was subject to restoration measures in the 1970s. The DHM behaviour of the clones was observed both in the presence
and absence of fish kairomone. Significant interclonal variation in DHM behaviour occurred in both treatments. To study the
micro-evolutionary response of the Lake Ring D. magna population, two approaches were used. First, we compared the DHM behaviour of clones derived from ephippia collected at different
depths. A comparison was conducted between clones resurrected from the period of eutrophication (1960–1980) and from the period
of recovery (1986–2000). A significant treatment (presence and absence of fish kairomone) × period interaction effect was
identified, suggesting a significant micro-evolutionary response for DHM behaviour. The D. magna clones exhibited a significantly stronger horizontal migration response during the period of eutrophication than in the recovery
phase. Second, clonal means, representing the influence of the genotype on the trait, were correlated with environmental conditions
(macrophyte cover, fish predation pressure and Secchi depth). The results of this analysis also suggest that a micro-evolutionary
response by Daphnia has occurred in reaction to changes in fish predation pressure. In periods with high fish predation pressure, Daphnia migrated more strongly towards the plants.
Guest editor: Piet Spaak
Cladocera: Proceedings of the 7th International Symposium on Cladocera 相似文献