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81.
Aboul-Enein HY Kladna A Kruk I Lichszteld K Marchlewicz M Michalska T Salem OI Hartmann RW 《Biopolymers》2004,73(5):631-639
4-(4-Phenoxybenzoyl)benzoic acid derivatives (PBADs) were found to inhibit rat and human alpha-reductase isozymes 1 and 2 in vitro. Chemiluminescence (CL), electron spin resonance, spin trapping techniques, and spectrophotometry were used to examine the effect of PBADs on reactive oxygen species (superoxide radical, O(2)(.-); hydroxyl radical, HO(*); singlet oxygen, (1)O(2)) generating systems. All test compounds at a concentration of 0.5 mM enhanced the CL from O(2)(.-) up to fivefold, which was recorded as the light sums during 1 min. At 0.38 mM PBAD enhanced production of HO(*) from H(2)O(2) in the presence of Co(II) up to 90%, as measured by a deoxyribose assay. Using the spin trap agent 5,5-dimethyl-1-pyrroline-N-oxide, it was found that the amplitude of the signal arising from the Fenton-like reaction [Co(II)/H(2)O(2)] was significantly diminished by the test compounds. The compounds also inhibited the (1)O(2) dependent 2,2,6,6-tetramethylpiperidine-N-oxide radical, which is generated in the acetonitrile/H(2)O(2) system. The measured rate constants of (1)O(2)-dimol quenching by PBAD were in the range of (0.8-2.6) x 10(8) M(-1) s(-1). The interaction between PBAD and (1)O(2) was also checked using a spectrophotometry method based on bleaching of p-nitrosodimethylaniline. These results indicate that PBAD may directly scavenge HO(*) and (1)O(2), but not O(2)(.-). However, the compounds that were examined had prooxidant ability under some reaction conditions. 相似文献
82.
Cross validation (CV) was used to analyze the effects of different environments and different genotypic samples on estimates of the proportion of genotypic variance explained by QTL (p). Testcrosses of 344 F(3) maize lines grown in four environments were evaluated for a number of agronomic traits. In each of 200 replicated CV runs, this data set was subdivided into an estimation set (ES) and various test sets (TS). ES were used to map QTL and estimate p for each run (p(ES)) and its median (p(ES)) across all runs. The bias of these estimates was assessed by comparison with the median (p(TS.ES)) obtained from TS. We also used two independent validation samples derived from the same cross for further comparison. The median p(ES) showed a large upward bias compared to p(TS.ES). Environmental sampling generally had a smaller effect on the bias of p(ES) than genotypic sampling or both factors simultaneously. In independent validation, p(TS.ES) was on average only 50% of p(ES). A wide range among p(ES) reflected a large sampling error of these estimates. QTL frequency distributions and comparison of estimated QTL effects indicated a low precision of QTL localization and an upward bias in the absolute values of estimated QTL effects from ES. CV with data from three QTL studies reported in the literature yielded similar results as those obtained with maize testcrosses. We therefore recommend CV for obtaining asymptotically unbiased estimates of p and consequently a realistic assessment of the prospects of MAS. 相似文献
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Katarzyna Michalska Anna Skoracka Denise Navia James W. Amrine 《Experimental & applied acarology》2010,51(1-3):31-59
Eriophyoid mites are excellent candidates for ethological research using the approaches of behavioural ecology and sociobiology. These tiny haplodiploid mites are highly specialized plant parasites, producing galls, forming nests, inhabiting refuges or living freely on plants. They reproduce via spermatophores deposited on a substrate and without pairing, which is a fascinating, though still poorly understood, mode of reproduction widespread in some groups of arthropods. Eriophyoid males can be involved in external sperm competition. In some species they also guard pre-emergent females and deposit spermatophores beside them. Although slow-walking, the minute eriophyoid mites can disperse for long distances on air currents or specific animal carriers. After landing on a plant they can distinguish between suitable and unsuitable hosts. Biological observations on a deuterogynous species indicate that parasociality could occur among eriophyoid mites. Many eriophyoids are of economic importance. Knowledge of their behaviour may promote understanding their ecology, may resolve problems in their phylogeny and may help developing methods for their control. In this paper, attention is directed to dispersal modes of eriophyoid mites, their feeding and host acceptance, spermatophore deposition and mating, defence against predators, and social behaviour. 相似文献
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A simple and rapid isocratic reversed-phase high-performance liquid chromatographic method for the quantification of alantolactone/isoalantolactone and three thymol derivatives in roots and root cultures of Inula helenium and I. royleana has been developed. The method could be applied to screen raw materials in search for highly productive plants and in vitro cultures. 相似文献
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