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31.
During fall migration many songbirds switch from consuming primarily insects to consuming mostly fruit. Fruits with more carbohydrates and less protein may be sufficient to rebuild expended fat stores, but such fruits may be inadequate to replace catabolized protein. We manipulated the concentrations and isotopic signatures of macronutrients in diets fed to birds to study the effects of diet quality on metabolic routing of dietary nutrients. We estimated that approximately 45% and 75%, respectively, of the carbon in proteinaceous tissue of birds switched to high- or low-protein diets came from nonprotein dietary sources. In contrast, we estimated that approximately 100% and 20%-80%, respectively, of the nitrogen in proteinaceous tissues of birds switched to high- or low-protein diets was attributable to dietary protein. Thus, the routing and assimilation of dietary carbon and nitrogen differed depending on diet composition. As a result, delta (15)N of tissues collected from wild animals that consume high-quality diets may reliably indicate the dietary protein source, whereas delta (13)C of these same tissues is likely the product of metabolic routing of carbon from several macronutrients. These results have implications for how isotopic discrimination is best estimated and how we can study macronutrient routing in wild animals. 相似文献
32.
BRCA1 regulates the interferon gamma-mediated apoptotic response 总被引:3,自引:0,他引:3
33.
Lock RA Coombs GW McWilliams TM Pearman JW Grubb WB Melrose GJ Forbes GM 《Helicobacter》2002,7(3):175-182
Background. Identification of the immunoreactive proteins of Helicobacter pylori is important for the development of both diagnostic tests and vaccines relating to the organism. Our aim was to determine whether there are significant differences between human IgG and IgA reactivities to individual H. pylori proteins, and whether patterns of immunoreactivity are sustained across different strains of H. pylori. Method. The total complement of protein from seven strains of H. pylori was resolved by two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE). Proteins were transferred electrophoretically onto polyvinylene difluoride (PVDF) membranes, which were probed with sera pooled either from H. pylori‐infected patients, or noninfected (control) patients. Highly immunoreactive proteins were detected using chromogenic enzyme‐antibody conjugates recognising either serum IgG or IgA. These proteins were then characterised by tryptic peptide‐mass fingerprinting using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS). Results. Highly immunoreactive proteins were detected which were common to all seven strains, and recognised by both immunoglobulin subclasses. The proteins appear to be localised in five groups. Protein analysis established that these groups encompass multiple isoforms of chaperonin HspB (two subgroups); urease β‐subunit UreB; elongation factor EF‐Tu; and flagellin FlaA. The pattern of highly immunoreactive proteins was strongly conserved across the seven strains. Conclusion. These results suggest that within a tightly defined region on the H. pylori proteome map there are five groups of proteins that are highly reactive to both IgG and IgA. Our analysis suggests it is unlikely that the highly immunoreactive clusters harbour any significant proteins other than isoforms of HspB, UreB, EF‐Tu and FlaA, and that, with the partial exception of FlaA, these clusters are strongly conserved across all seven strains. 相似文献
34.
Test of a digestion optimization model: effect of variable-reward feeding schedules on digestive performance of a migratory bird 总被引:1,自引:0,他引:1
Birds on migration often alternate between feeding and nonfeeding periods, in part because food resources may be patchily
distributed and in part because birds on migration may adopt a risk-prone foraging strategy characterized by selection of
variable rather than constant food rewards. Optimal digestion models predict that increases in intermeal interval like those
encountered by some migratory birds should result in longer retention time of digesta and higher digestive efficiency if birds
are maximizing their rate of energy intake. We tested these predictions by comparing residence time of digesta and extraction
efficiency of lipid for captive yellow-rumped warblers (Dendroica coronata) feeding adlibitum and when we added intervals of time when the birds received no food. We increased the likelihood that the warblers were maximizing
their rate of energy intake by increasing light levels during spring to induce hyperphagia (treatment birds (16L:8D light:
dark cycle) ate 2.13 ± 0.14 g dry food day−1 (n = 8) while control birds (10L:14D) ate 1.25 ± 0.03 g dry food day−1 (n = 6)). Treatment birds offered food only every other 2–3 h ate 50% more during the 4-h test period than when they were always
feeding adlibitum. Despite these differences in food intake, extraction efficiency of glycerol trioleate remained high and constant (93%),
and mouth-to-anus total mean retention time (TMRT) did not change (overall mean: 54.8 ± 6.0 min). Residence time of lipid
in the stomach increased whereas residence time of lipid in the intestine decreased when birds fed only every other 2–3 h
compared to when birds always fed ad libitum. None of the results were consistent with the predictions of the optimal digestion model unless we assume that birds were
minimizing their feeding time rather than maximizing their rate of energy gain. Furthermore, the ability of yellow-rumped
warblers to maintain high extraction efficiency with no change in TMRT suggests some spare digestive capacity when food intake
increases by as much as 50%.
Received: 14 June 1997 / Accepted: 20 November 1997 相似文献
35.
Quantum-dye labeled proteins for glycobiology: a viable nonradioactive alternative tracer 总被引:1,自引:1,他引:0
Quantum dye (QD), a macrocyclic europium-chelate, developed as a
cytological marker, has never been used for quantitative applications. It
would be ideal, however, if the same tracer can be used for both
qualitative and quantitative purposes. We have labeled some lectins and
neoglycoproteins with QD for the purpose of quantitative analyses in
glycobiology, and tested its suitability in three different areas in
glycobiology: (1) glycosyltransferase, (2) an animal lectin - mannose-
binding protein, and (3) the Gal/GalNAc receptor of rat liver membrane.
Usefulness of QD-labeled lectins was amply demonstrated by the
quantification of galactosyltransferase activity using QD-soybean
agglutinin and QD-RCA120 ( Ricinus communis agglutinin). We also showed
that QD-labeled neoglycoproteins, QD-Man-BSA and QD-Gal-BSA, can replace
radioiodinated counterparts in the binding assays of animal lectins (serum
mannose binding protein and hepatic Gal/GalNAc receptor.) The advantage of
QD and other europium labels is that it does not decay as radioiodides do.
The long shelf-life results in more consistent results from repeated
experiments.
相似文献
36.
P. G. McWilliams W. T. W. Potts 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1978,126(3):277-286
Summary Measurements of the transepithelial potential (Vint-Vext) across the gills of Brown Trout,Salmo trutta, were made in solutions of a range of pH and calcium concentrations. The potential was strongly dependent on external pH, being negative in neutral solutions but positive in acid solutions. The addition of calcium to the external medium produced a positive shift in potential in all but very acid media (pH 4.0–3.5), where very little change was seen. The gill membrane appears to act as a hydrogen electrode having a very high permeability to H+ ions, and the potential behaves as a diffusion potential. The presence of calcium reduced the permeability to both H+ and Na+ ions but even at a calcium concentration of 8.0 mM/l the permeability ratio H+/Na+ was still more than 900. The transepithelial potential is shown to be diffusional in origin and is discussed in terms of the relative permeability of the gill to H+, Na+ and Cl– ions. Sodium fluxes across the gills were measured and provide the basis for a theoretical consideration of Na+, Cl– and H+ fluxes across the gills in neutral and acid solutions. The positive potential at low pH largely accounts for the increased loss of sodium from fish in these conditions. 相似文献
37.
38.
Palatal length in cleft palate as a predictor of speech outcome 总被引:1,自引:0,他引:1
Randall P LaRossa D McWilliams BJ Cohen M Solot C Jawad AF 《Plastic and reconstructive surgery》2000,106(6):1254-9; discussion 1260-1
In an attempt to predict which patients might benefit from primary posterior pharyngeal flaps done at the time of palatal repair, palatal length was assessed before palatal repair and the patient was placed in one of four categories. Patients with longer palates preoperatively had statistically better speech outcomes than patients with shorter palates. Statistical significance was found for most speech parameters. Information on presurgical palatal length can be useful in predicting which patients might profit from primary "pharyngoplasties." 相似文献
39.
Stable isotopes in breath,blood, feces and feathers can indicate intra-individual changes in the diet of migratory songbirds 总被引:5,自引:0,他引:5
We used stable isotopes of C in breath, blood, feces and feathers to identify intra-individual changes in diet and the timescale of diet changes in free-living songbirds at a stopover site. Because accurate interpretation of differences between the 13C of breath, plasma, and red blood cells (RBCs) relative to diet requires knowing the turnover rate of C within them, we determined the rate of change of C in breath, plasma and RBCs for yellow-rumped warblers (Dendroica coronata). Half-lives of C in breath, plasma, and RBCs were 4.4±2.1 h, 24.8±12.3 h and 10.9±3.2 days, respectively, for yellow-rumped warblers. 13C of breath, plasma, RBCs and feces from wild-caught golden-crowned kinglets (Regulus satrapa), ruby-crowned kinglets (R. calendula) and gray catbirds (Dumetella carolinensis) indicated that they had maintained an isotopically consistent diet for an extended period of time. However, 13C of breath and plasma indicated that white-throated sparrows (Zonotrichia albicollis) had recently expanded their diet to include a C4 dietary component. Likewise, 13C of breath, plasma, RBCs and feces indicated that some wild-caught yellow-rumped warblers had consumed foods with a more enriched protein signature prior to their arrival on Block Island, and since arrival, they had consumed mostly northern bayberry (Myrica pensylvanica). Therefore, comparisons of the 13C of breath, plasma, RBCs, feces and feathers from individual songbirds can indicate changes in diet and provide an estimate of the timescale of the diet change. 相似文献
40.