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11.
PROPERTIES AND METABOLISM OF SOLUBLE LIPOPROTEINS CONTAINING CHOLINE AND ETHANOLAMINE PHOSPHOLIPIDS IN RAT BRAIN 总被引:1,自引:0,他引:1
Abstract— Choline and ethanolamine phospholipids in the 105,000 g supernatant fraction of rat brain exhibited density and electrophoretic properties consistent with their binding to protein. About 40% of these two phospholipids were bound to soluble lipoproteins, whereas the remainder appeared to be associated with particulate complexes. Following intracranial injection of [2-3 H]glycerol, the specific radioactivities of the choline and ethanolamine phospholipids in the supernatant fraction were higher than those in the microsomal fraction at all time points examined, from 15 min to 12 h after injection. The properties of cytoplasmic lipoproteins containing choline and ethanolamine phospholipids have been compared with those which we have previously described containing sulphatide. 相似文献
12.
[2-3 H]GLYCEROL AS A PRECURSOR OF PHOSPHOLIPIDS IN RAT BRAIN: EVIDENCE FOR LACK OF RECYCLING 总被引:1,自引:0,他引:1
Abstract— When [2-3 H]glycerol was injected intracranially into young rats, it was presented as a pulse label, leaving the brain rapidly and giving up much of its labelled hydrogen to water. [2-3 H]glycerol was efficiently incorporated into brain lipids, especially into choline and ethanolamine phospholipids. Following injection of a mixture of [3 H]- and [14 C]-labelled glycerol, the ratio of 3 H to 14 C in the phospholipids of both whole brain and the microsomal fraction decreased as a function of time after injection. This finding indicated less recycling of the tritium label. This lack of recycling was further indicated by the finding that 94 per cent of the tritium label of phosphatidyl choline was in the glycerol portion of the molecule rather than in the fatty acids. At 2 weeks following injection with [3 H]glycerol, 93 per cent of the total radioactivity in brain appeared in the lipid fraction. In contrast, following injection with [14 C]glycerol, only 57 per cent of the radioactivity appeared in lipid, with about 20 per cent in protein. 相似文献
13.
Light-scattering patterns of isolated oligodendroglia 总被引:1,自引:0,他引:1
14.
Studies of water soluble lipoproteins in rat brain 总被引:3,自引:2,他引:1
—The occurrencc of water soluble lipoprotein in the 105,000 g supernatant of rat brain homogenate has been demonstrated. The lipoproteins of brain differed from those of the serum with respect to lipid composition, density and reaction to serum lipoprotein antibodies. Acrylamide gel electrophoresis resolved the brain supernatant into four zones which stained with lipid dye. It was possible to demonstrate that these zones also contained protein by double staining of the gel for lipid and protein. Two of the zones differed in their mobility from serum lipoprotein. Preparative acrylamide gel electrophoresis isolated protein fractions in supematant which contained both lipid and protein and permitted preliminary identification of the lipoprotein bands amongst all the protein bands resolved by acrylamide gel electrophoresis. 相似文献
15.
M. Guarnieri H. Syed W. Weintraub G.M. McKhann 《Archives of biochemistry and biophysics》1975,167(2):581-587
Phospholipase C and D hydrolyze the membrane-bound phospholipids of isolated, untreated myelin. When the membrane is treated with detergents or solvents which disrupt the membrane structure, the activity of the enzymes against the membrane-bound lipids increases. Myelin in the central nervous system is derived from the cell membrane of the oligodendroglial cell. Because the phospholipids in erythrocyte cell membranes are strikingly resistent to phospholipase C and D hydrolysis the question is raised of whether myelin in situ, as opposed to isolated myelin, is susceptible to phospholipase hydrolysis. 相似文献
16.
This paper describes a new, sensitive, and reproducible method for the determination of 2′,3′-cyclic-nucleotide 3′-phosphodiesterase (EC 3.1.4.37) activity in both central and peripheral nervous system tissue, as well as in extraneural tissue. Radioactive [8-3H]adenosine 2′,3′-cyclic monophosphate was used as the substrate. The [8-3H]2′-AMP product formed was isolated by thin-layer chromatography, or, alternatively, the reaction was coupled with an excess of Escherichia coli alkaline phosphatase, and the [8-3H]adenosine formed was isolated by column chromatography on AG 1-X2 resin. The values obtained by the two methods were compared with those obtained using a spectrophotometric method. Hydrolysis rates of 0.50 nmol/min could be reproducibly measured in 18-day fetal rat spinal cord. 相似文献
17.
18.
Natural variation in CBF gene sequence,gene expression and freezing tolerance in the Versailles core collection of Arabidopsis thaliana 总被引:1,自引:0,他引:1
19.
Neurogenesis in the Adult Mammalian Brain 总被引:1,自引:0,他引:1
Sosunov A. A. Chelyshev Yu. A. McKhann G. Kruglyakov P. P. Balykova O. P. Shikhanov N. P. 《Russian Journal of Developmental Biology》2002,33(6):327-341
The concept of the CNS cell composition stability has recently undergone significant changes. It was earlier believed that neurogenesis in the mammalian CNS took place only during embryonic and early postnatal development. New approaches make it possible to prove that neurogenesis takes part even in the adult brain. The present review summarizes the data about the neural stem cell. It has been demonstrated that new neurons are constantly formed in adult mammals, including man. In two brain zones, subventricular zone and dentate gyrus, neurogenesis appears to proceed throughout the entire life of mammals, including man. The newly arising neurons are essential for some important processes, such as memory and learning. Stem cells were found in the subependymal and/or ependymal layer. They express nestin and have a low mitotic activity. During embryogenesis, the stem cell divides asymmetrically: one daughter cell resides as the stem cell in the ependymal layer and another migrates to the subventricular zone. There it gives rise to a pool of dividing precursors, from which neural and glial cells differentiate and migrate to the sites of final localization. The epidermal and fibroblast growth factors act as mitogens for the neural stem cell. The neural stem cell gives rise to the cells of all germ layers in vitro and has a wide potential for differentiation in the adult organism. Hence, it can be used as a source of various cell types of the nervous tissue necessary for cellular transplantation therapy. 相似文献
20.