全文获取类型
收费全文 | 556篇 |
免费 | 104篇 |
出版年
2021年 | 8篇 |
2019年 | 5篇 |
2017年 | 7篇 |
2016年 | 8篇 |
2015年 | 14篇 |
2014年 | 23篇 |
2013年 | 19篇 |
2012年 | 13篇 |
2011年 | 24篇 |
2010年 | 19篇 |
2009年 | 15篇 |
2008年 | 13篇 |
2007年 | 14篇 |
2006年 | 15篇 |
2005年 | 21篇 |
2004年 | 16篇 |
2003年 | 18篇 |
2002年 | 21篇 |
2001年 | 13篇 |
2000年 | 13篇 |
1999年 | 13篇 |
1998年 | 17篇 |
1997年 | 17篇 |
1996年 | 17篇 |
1995年 | 13篇 |
1994年 | 9篇 |
1993年 | 11篇 |
1992年 | 12篇 |
1991年 | 18篇 |
1990年 | 15篇 |
1989年 | 12篇 |
1988年 | 11篇 |
1987年 | 6篇 |
1986年 | 8篇 |
1985年 | 19篇 |
1984年 | 12篇 |
1983年 | 15篇 |
1982年 | 12篇 |
1981年 | 7篇 |
1979年 | 8篇 |
1978年 | 7篇 |
1977年 | 11篇 |
1976年 | 15篇 |
1975年 | 8篇 |
1974年 | 9篇 |
1973年 | 7篇 |
1972年 | 11篇 |
1971年 | 5篇 |
1970年 | 6篇 |
1969年 | 5篇 |
排序方式: 共有660条查询结果,搜索用时 390 毫秒
91.
Effects of chromosome-specific introgression in upland cotton on fiber and agronomic traits 总被引:9,自引:0,他引:9 下载免费PDF全文
Saha S Jenkins JN Wu J McCarty JC Gutiérrez OA Percy RG Cantrell RG Stelly DM 《Genetics》2006,172(3):1927-1938
Interspecific chromosome substitution is among the most powerful means of introgression and steps toward quantitative trait locus (QTL) identification. By reducing the genetic "noise" from other chromosomes, it greatly empowers the detection of genetic effects by specific chromosomes on quantitative traits. Here, we report on such results for 14 cotton lines (CS-B) with specific chromosomes or chromosome arms from G. barbadense L. substituted into G. hirsutum and chromosome-specific F2 families. Boll size, lint percentage, micronaire, 2.5% span length, elongation, strength, and yield were measured by replicated field experiments in five diverse environments and analyzed under an additive-dominance (AD) genetic model with genotype and environment interaction. Additive effects were significant for all traits and dominance effects were significant for all traits except 2.5% span length. CS-B25 had additive effects increasing fiber strength and fiber length and decreasing micronaire. CS-B16 and CS-B18 had additive effects related to reduced yields. The results point toward specific chromosomes of G. barbadense 3-79 as the probable locations of the genes that significantly affect quantitative traits of importance. Our results provided a scope to analyze individual chromosomes of the genome in homozygous and heterozygous conditions and thus detected novel effects of alleles controlling important QTL. 相似文献
92.
RP Tucker K Drabikowski JF Hess J Ferralli R Chiquet-Ehrismann JC Adams 《BMC evolutionary biology》2006,6(1):60-17
Background
Tenascins are a family of glycoproteins found primarily in the extracellular matrix of embryos where they help to regulate cell proliferation, adhesion and migration. In order to learn more about their origins and relationships to each other, as well as to clarify the nomenclature used to describe them, the tenascin genes of the urochordate Ciona intestinalis, the pufferfish Tetraodon nigroviridis and Takifugu rubripes and the frog Xenopus tropicalis were identified and their gene organization and predicted protein products compared with the previously characterized tenascins of amniotes. 相似文献93.
Arginine 352 (R352) in the sixth transmembrane domain of the cystic fibrosis transmembrane conductance regulator (CFTR) previously
was reported to form an anion/cation selectivity filter and to provide positive charge in the intracellular vestibule. However,
mutations at this site have nonspecific effects, such as inducing susceptibility of endogenous cysteines to chemical modification.
We hypothesized that R352 stabilizes channel structure and that charge-destroying mutations at this site disrupt pore architecture,
with multiple consequences. We tested the effects of mutations at R352 on conductance, anion selectivity and block by the
sulfonylurea drug glipizide, using recordings of wild-type and mutant channels. Charge-altering mutations at R352 destabilized
the open state and altered both selectivity and block. In contrast, R352K-CFTR was similar to wild-type. Full conductance
state amplitude was similar to that of wild-type CFTR in all mutants except R352E, suggesting that R352 does not itself form
an anion coordination site. In an attempt to identify an acidic residue that may interact with R352, we found that permeation
properties were similarly affected by charge-reversing mutations at D993. Wild-type-like properties were rescued in R352E/D993R-CFTR,
suggesting that R352 and D993 in the wild-type channel may interact to stabilize pore architecture. Finally, R352A-CFTR was
sensitive to modification by externally applied MTSEA+, while wild-type and R352E/D993R-CFTR were not. These data suggest that R352 plays an important structural role in CFTR,
perhaps reflecting its involvement in forming a salt bridge with residue D993. 相似文献
94.
95.
Winton MJ Van Deerlin VM Kwong LK Yuan W Wood EM Yu CE Schellenberg GD Rademakers R Caselli R Karydas A Trojanowski JQ Miller BL Lee VM 《FEBS letters》2008,582(15):2252-2256
TAR DNA-binding protein-43 (TDP-43) is a highly conserved, ubiquitously expressed nuclear protein that was recently identified as the disease protein in frontotemporal lobar degeneration with ubiquitin-positive inclusions (FTLD-U) and amyotrophic lateral sclerosis (ALS). Pathogenic TDP-43 gene (TARDBP) mutations have been identified in familial ALS kindreds, and here we report a TARDBP variant (A90V) in a FTLD/ALS patient with a family history of dementia. Significantly, A90V is located between the bipartite nuclear localization signal sequence of TDP-43 and the in vitro expression of TDP-43-A90V led to its sequestration with endogenous TDP-43 as insoluble cytoplasmic aggregates. Thus, A90V may be a genetic risk factor for FTLD/ALS because it predisposes nuclear TDP-43 to redistribute to the cytoplasm and form pathological aggregates. 相似文献
96.
97.
Two highly conserved amino acid residues, an arginine and a glutamine, located near the C-terminal end of the gamma subunit, form a "catch" by hydrogen bonding with residues in an anionic loop on one of the three catalytic beta subunits of the bovine mitochondrial F1-ATPase [Abrahams, J. P., Leslie, A. G., Lutter, R., and Walker, J. E. (1994) Nature 370, 621-628]. The catch is considered to play a critical role in the binding change mechanism whereby binding of ATP to one catalytic site releases the catch and induces a partial rotation of the gamma subunit. This role is supported by the observation that mutation of the equivalent arginine and glutamine residues in the Escherichia coli F1 gamma subunit drastically reduced all ATP-dependent catalytic activities of the enzyme [Greene, M. D., and Frasch, W. D. (2003) J. Biol. Chem. 278, 5194-5198]. In this study, we show that simultaneous substitution of the equivalent residues in the chloroplast F1 gamma subunit, arginine 304 and glutamine 305, with alanine decreased the level of proton-coupled ATP synthesis by more than 80%. Both the Mg2+-dependent and Ca2+-dependent ATP hydrolysis activities increased by more than 3-fold as a result of these mutations; however, the sulfite-stimulated activity decreased by more than 60%. The Mg2+-dependent, but not the Ca2+-dependent, ATPase activity of the double mutant was insensitive to inhibition by the phytotoxic inhibitor tentoxin, indicating selective loss of catalytic cooperativity in the presence of Mg2+ ions. The results indicate that the catch residues are required for efficient proton coupling and for activation of multisite catalysis when MgATP is the substrate. The catch is not, however, required for CaATP-driven multisite catalysis or, therefore, for rotation of the gamma subunit. 相似文献
98.
99.
100.