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951.
952.
We investigated how the audience member’s physiological reactions differ as a function of listening context (i.e., live versus recorded music contexts). Thirty-seven audience members were assigned to one of seven pianists’ performances and listened to his/her live performances of six pieces (fast and slow pieces by Bach, Schumann, and Debussy). Approximately 10 weeks after the live performance, each of the audience members returned to the same room and listened to the recorded performances of the same pianists’ via speakers. We recorded the audience members’ electrocardiograms in listening to the performances in both conditions, and analyzed their heart rates and the spectral features of the heart-rate variability (i.e., HF/TF, LF/HF). Results showed that the audience’s heart rate was higher for the faster than the slower piece only in the live condition. As compared with the recorded condition, the audience’s sympathovagal balance (LF/HF) was less while their vagal nervous system (HF/TF) was activated more in the live condition, which appears to suggest that sharing the ongoing musical moments with the pianist reduces the audience’s physiological stress. The results are discussed in terms of the audience’s superior attention and temporal entrainment to live performance. 相似文献
953.
Massimilano Scolz Per O. Widlund Silvano Piazza Debora Rosa Bublik Simone Reber Leticia Y. Peche Yari Ciani Nina Hubner Mayumi Isokane Martin Monte Jan Ellenberg Anthony A. Hyman Claudio Schneider Alexander W. Bird 《PloS one》2012,7(12)
The regulation of cell migration is a highly complex process that is often compromised when cancer cells become metastatic. The microtubule cytoskeleton is necessary for cell migration, but how microtubules and microtubule-associated proteins regulate multiple pathways promoting cell migration remains unclear. Microtubule plus-end binding proteins (+TIPs) are emerging as important players in many cellular functions, including cell migration. Here we identify a +TIP, GTSE1, that promotes cell migration. GTSE1 accumulates at growing microtubule plus ends through interaction with the EB1+TIP. The EB1-dependent +TIP activity of GTSE1 is required for cell migration, as well as for microtubule-dependent disassembly of focal adhesions. GTSE1 protein levels determine the migratory capacity of both nontransformed and breast cancer cell lines. In breast cancers, increased GTSE1 expression correlates with invasive potential, tumor stage, and time to distant metastasis, suggesting that misregulation of GTSE1 expression could be associated with increased invasive potential. 相似文献
954.
Hiroshi Yamasaki Taira Enomoto Nicole Martel Yoshiki Shiba Yoshinobu Kanno 《Experimental cell research》1983,146(2)
A tumour promoter, 12-O-tetradecanoyl phorbol-13-acetate (TPA), reversibly inhibits the onset and maintenance of cell-cell communication measured by electrophysiological method. We have now studied the mechanism by which TPA inhibits communication of human cells (FL) in culture. Using [3H]phorbol-12,13-dibutyrate ([3H]PDBu), we found a class of specific, high-affinity, saturable binding sites in intact FL cells; they have a dissociation constant of 15.4 nM, and at saturation about 3 × 105 PDBu molecules were bound to each cell. The binding of [3H]PDBu to FL cells was inhibited by TPA, phorbol-12-13-didecanoate and mezerein, whereas phorbol and 4α-phorbol-12-13-didecanoate had no effect. There is a close correlation between the ability of the former compounds to inhibit [3H]PDBu binding and their capacity to inhibit cell-cell communication. When FL cells are dispersed with EDTA and plated onto a culture dish, they start to couple electrically within 2 h; such cell coupling was not affected by the presence of cycloheximide or actinomycin D. TPA inhibits the formation of electrical cell coupling as well as its maintenance, even in the presence of cycloheximide; the recovery of cell-cell communication after the removal of TPA was not significantly affected by the addition of cycloheximide or actinomycin D. Taken together, these results suggest that TPA-mediated reversible inhibition of intercellular communication is mediated by specific binding of TPA to cellular receptors and that macromolecular synthesis is not necessary. 相似文献
955.
Gunji Mamiya Kunio Takishima Mayumi Masakuni Tatsuko Kayumi Kazuko Ogawa 《Journal of Protein Chemistry》1987,6(1):55-59
The subunit structure of jack bean urease has been unresolved in spite of many investigations. Thus far, the molecular weight for the native urease seem to range from 480,000 to 590,000 and the values for the monomer range from 30,000 to 97,000. The complete amino acid sequence of jack bean urease has been determined primarily by sequencing cyanogen bromide peptides, which were aligned by overlapping peptides obtained by lysylendopeptidase digestion of the protein and tryptic digestion of the citraconylated protein. The protein contains 840 amino acid residues in a single polypeptide chain and the subunit molecular weight calculated from the sequence is 90,790. The value of 544,740 for the hexamer, consistent with the value of 580,000 determined for intact urease by centrifugal analyses, indicated that urease consists of six subunits. Thirteen of 25 histidine residues in the urease subunit are crowded in the region between residues 479 and 607. Urease is a nickel metalloenzyme and the nickel has an essential role in catalysis by this enzyme. It is noteworthy that cysteine-592, which is recognized as essential for enzymatic activity and is related to the nickel ion in the active center, is located on this histidine-rich sequence.This article was presented during the proceedings of the International Conference on Macromolecular Structure and Function, held at the National Defence Medical College, Tokorozawa, Japan, December 1985. 相似文献
956.
Yumiko Kirihara Mayumi Takechi Kaoru Kurosaki Yuta Kobayashi Yoji Saito Takashi Takeuchi 《Experimental Animals》2016,65(1):27-36
An anesthetic mixture of medetomidine (MED), midazolam (MID), and butorphanol (BUT) has
been used in laboratory animals. We previously reported that this anesthetic mixture
produced closely similar anesthetic effects in BALB/c and C57BL/6J strains. We also
demonstrated the efficacy of atipamezole (ATI), an antagonist of MED that produced quick
recovery from anesthesia in mice. Anesthetics have various anesthetic effects among animal
strains. However, the differences in the effects of anesthetic mixtures in rats are
unclear. In the present study, we first examined effects of the abovementioned anesthetic
mixture using three different rat strains: Wistar (WST), Sprague-Dawley (SD), and Fischer
344 (F344). Second, we examined how different dosages and optimum injection timing of ATI
affected recovery from anesthesia in rats. We used the anesthetic score to measure
anesthetic duration and a pulse oximeter to monitor vital signs. We found no significant
differences in anesthetic duration among the three different strains. However, recovery
from anesthesia in the SD strain took significantly longer than in the other strains. The
antagonistic effects of ATI (0.15 mg/kg and 0.75 mg/kg) were equivalent when administered
at 30 min after anesthetic mixture administration. The antagonistic effects of ATI 0.75
mg/kg were stronger than those of ATI 0.15 mg/kg at 10 min after anesthetic mixture
administration. This anesthetic mixture is a useful drug that can induce similar
anesthetic effects in three different strains and has an antagonist, ATI, that makes rats
quickly recover from anesthesia. These results may contribute to the welfare of laboratory
animals. 相似文献
957.
Borges Aline Chiodi Nishime Thalita Mayumi Castaldelli de Moura Rovetta Sabrina Lima Gabriela de Morais Gouvêa Kostov Konstantin Georgiev Thim Gilmar Patrocínio de Menezes Beatriz Rossi Canuto Machado João Paulo Barros Koga-Ito Cristiane Yumi 《Mycopathologia》2019,184(5):585-595
Mycopathologia - This study aimed to evaluate the effects of cold atmospheric pressure plasma (CAPP) jet on Trichophyton rubrum growth, germination and adherence to nail. The effects of... 相似文献
958.
The oncoprotein Bcl-3 can facilitate NF-kappa B-mediated transactivation by removing inhibiting p50 homodimers from select kappa B sites. 总被引:11,自引:3,他引:8
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G Franzoso V Bours V Azarenko S Park M Tomita-Yamaguchi T Kanno K Brown U Siebenlist 《The EMBO journal》1993,12(10):3893-3901
Previously we have proposed a role for Bcl-3 in facilitating transactivation through kappa B sites by counteracting the inhibitory effects of bound, non-transactivating homodimers of the p50 subunit of NF-kappa B. Such homodimers are abundant for example in nuclei of unstimulated primary T cells. Here we extend the model and provide new evidence which fulfills a number of predictions. (i) Bcl-3 preferentially targets p50 homodimers over NF-kappa B heterodimers since the homodimers are completely dissociated from kappa B sites at concentrations of Bcl-3 which do not affect NF-kappa B. (ii) Select kappa B sites associate very strongly and stably with p50 homodimers, completely preventing binding by NF-kappa B. Such kappa B sites are likely candidates for regulation by p50 homodimers and Bcl-3. (iii) Bcl-3 and p50 can be co-localized in the nucleus, a requirement for active removal of homodimers from their binding sites in vivo. (iv) The ankyrin repeat domain of Bcl-3 is sufficient for the reversal of p50 homodimer-mediated inhibition, correlating with the ability of this domain alone to inhibit p50 binding to kappa B sites in vitro. Our data support the model that induction of nuclear Bcl-3 may be required during cellular stimulation to actively remove stably bound p50 homodimers from certain kappa B sites in order to allow transactivating NF-kappa B complexes to engage. This exact mechanism is demonstrated with in vitro experiments. 相似文献
959.
We have investigated the effects of various types of collagen and a tumor-promoting phorbol ester on intercellular contacts and the organization of actin in human amnion epithelial FL cells and mouse fibroblast 3T3-A31 cells. Our purpose was to investigate how modulation of interactions between cells and the substratum leads to alterations in intercellular contacts and organization of actin filaments. When cells were cultured on dishes coated with a solution containing type I collagen, but not type IV, changes were induced in the morphology of FL cells and their intercellular contacts. Type I collagen also caused changes in the organization of their actin filaments, although no such effects were observed with 3T3-A31 cells. In contrast, 12-O-tetradecanoylphorbol-13-acetate (TPA) caused morphological changes, dissociation of groups of cells, and reorganization of actin filaments in cultures of FL and 3T3-A31 cells. It also disrupted the sites of adhesion of FL cells to the substratum. Both type I collagen and TPA rapidly induced spreading of FL cells in the absence of serum. However, cis-hydroxyproline, known to inhibit secretion of collagen, did not suppress the TPA-induced dissociation of groups of FL cells. These results suggest that the interactions with type I collagen of epithelial FL cells, but not of fibroblastic 3T3-A31 cells, tend to disorganize cellular morphology, intercellular contacts, and actin filaments in ways similar to, but not directly related to, the effects of TPA. 相似文献
960.