全文获取类型
收费全文 | 313篇 |
免费 | 14篇 |
专业分类
327篇 |
出版年
2024年 | 1篇 |
2023年 | 4篇 |
2022年 | 2篇 |
2021年 | 7篇 |
2020年 | 5篇 |
2019年 | 6篇 |
2018年 | 5篇 |
2017年 | 10篇 |
2016年 | 5篇 |
2015年 | 12篇 |
2014年 | 21篇 |
2013年 | 22篇 |
2012年 | 31篇 |
2011年 | 29篇 |
2010年 | 17篇 |
2009年 | 19篇 |
2008年 | 16篇 |
2007年 | 19篇 |
2006年 | 20篇 |
2005年 | 20篇 |
2004年 | 14篇 |
2003年 | 20篇 |
2002年 | 11篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 4篇 |
1998年 | 2篇 |
1993年 | 1篇 |
1992年 | 1篇 |
排序方式: 共有327条查询结果,搜索用时 15 毫秒
31.
32.
Mayuko Jomura Yuhei Akashi Hiromu Itoh Risa Yuki Yoshimi Sakai Yutaka Maruyama 《PloS one》2015,10(12)
As a large, long-term pool and source of carbon and nutrients, woody litter is an important component of forest ecosystems. The objective of this study was to estimate the effect of the factors that regulate the rate of decomposition of coarse and fine woody debris (CFWD) of dominant tree species in a cool-temperate forest in Japan. Respiration rates of dead stems, branches, and coarse and fine roots of Fagus crenata and Quercus crispula felled 4 years prior obtained in situ ranged from 20.9 to 500.1 mg CO2 [kg dry wood]–1 h–1 in a one-time measurement in summer. Respiration rate had a significant negative relationship with diameter; in particular, that of a sample of Q. crispula with a diameter of >15 cm and substantial heartwood was low. It also had a significant positive relationship with moisture content. The explanatory variables diameter, [N], wood density, and moisture content were interrelated. The most parsimonious path model showed 14 significant correlations among 8 factors and respiration. Diameter and [C] had large negative direct effects on CFWD respiration rate, and moisture content and species had medium positive direct effects. [N] and temperature did not have direct or indirect effects, and position and wood density had indirect effects. The model revealed some interrelationships between controlling factors. We discussed the influence of the direct effects of explanatory variables and the influence especially of species and position. We speculate that the small R
2 value of the most parsimonious model was probably due to the omission of microbial biomass and activity. These direct and indirect effects and interrelationships between explanatory variables could be used to develop a process-based CFWD decomposition model. 相似文献
33.
Nakamura Shizuka Fukuma Koichi Wada Kazushige Ueda Mayuko Hayashi Mitsuo 《Sleep and biological rhythms》2018,16(3):351-356
Sleep and Biological Rhythms - The nocturnal sleep of train drivers is apt to be short and their lifestyle become irregular because they drive at midnight and early in the morning. As a result,... 相似文献
34.
35.
Temporal and spatial changes in gene expression,metabolite accumulation and phytohormone content in rice seedlings grown under drought stress conditions 下载免费PDF全文
Daisuke Todaka Yu Zhao Takuya Yoshida Madoka Kudo Satoshi Kidokoro Junya Mizoi Ken‐Suke Kodaira Yumiko Takebayashi Mikiko Kojima Hitoshi Sakakibara Kiminori Toyooka Mayuko Sato Alisdair R. Fernie Kazuo Shinozaki Kazuko Yamaguchi‐Shinozaki 《The Plant journal : for cell and molecular biology》2017,90(1):61-78
36.
Hisamitsu Ide Yan Lu Jingsong Yu Takahiro Noguchi Mayuko Kanayama Satoru Muto Raizo Yamaguchi Suguru Kawato Shigeo Horie 《Human cell》2017,30(2):133-139
There is now mounting evidence that the aryl hydrocarbon receptor (AhR) plays an important role in physiologic responses such as development, cell cycle regulation, immune function and also malignant transformation in various tissues. The strong nuclear AhR expression is observed in the invasive phenotype, and an elevated nuclear AhR expression is associated with a poor prognosis of human prostate cancer. On the other hand, there are conflicting results that the AhR deficiency results in increased susceptibility to prostate tumors in mouse model. In the present study, we investigated AhR expression and its role in the growth and invasiveness of human prostate cancer cells. The AhR protein expression was detected in prostate cancer cell lines and human prostate cancer tissues. A small interfering RNA targeting AhR, constitutive active AhR expression vector, and AhR agonist and antagonist were used to moderate its expression and signaling. The induction of AhR signaling attenuated invasiveness of prostate cancer cells without affecting the cellular growth rate. These results suggest that AhR signaling in prostate cancer cells facilitates invasion of these cells, and modulation with this signaling can be a potential therapeutic target of invasive tumors. 相似文献
37.
Planta - We identified LSG2 as a novel lytic enzyme that accumulates in the parental extracellular matrix and disrupts parental spheroids cooperatively with VheA secreted by juveniles in Volvox.... 相似文献
38.
Matsunari H Onodera M Tada N Mochizuki H Karasawa S Haruyama E Nakayama N Saito H Ueno S Kurome M Miyawaki A Nagashima H 《Cloning and stem cells》2008,10(3):313-324
Genetically engineered pigs with cell markers such as fluorescent proteins are highly useful in lines of research that include the tracking of transplanted cells or tissues. In this study, we produced transgenic-cloned pigs carrying a gene for the newly developed red fluorescent protein, humanized Kusabira-Orange (huKO), which was cloned from the coral stone Fungia concinna. The nuclear transfer embryos, reconstructed with fetal fibroblast cells that had been transduced with huKO cDNA using retroviral vector DDeltaNsap, developed efficiently in vitro into blastocysts (28.0%, 37/132). Nearly all (94.6%, 35/37) of the cloned blastocysts derived from the transduced cells exhibited clear huKO gene expression. A total of 429 nuclear transfer embryos were transferred to four recipients, all of which became pregnant and gave birth to 18 transgenic-cloned offspring in total. All of the pigs highly expressed huKO fluorescence in all of the 23 organs and tissues analyzed, including the brain, eyes, intestinal and reproductive organs, skeletal muscle, bone, skin, and hoof. Furthermore, such expression was also confirmed by histological analyses of various tissues such as pancreatic islets, renal corpuscles, neuronal and glial cells, the retina, chondrocytes, and hematopoietic cells. These data demonstrate that transgenic-cloned pigs exhibiting systemic red fluorescence expression can be efficiently produced by nuclear transfer of somatic cells retrovirally transduced with huKO gene. 相似文献
39.
40.
Production of Transgenic-clone Pigs by the Combination of ICSI-mediated Gene Transfer with Somatic Cell Nuclear Transfer 总被引:5,自引:0,他引:5
The objective of this study was to examine whether the ICSI-mediated gene transfer method using in vitro matured oocytes and frozen sperm head could actually produce transgenic pigs. We also aimed at examining whether transgenic
pigs can be cloned from somatic cells of a transgenic pig generated by the ICSI-mediated method. A bicistronic gene constituted
of the human albumin (hALB) and enhanced green fluorescent protein (EGFP) genes was introduced into pig oocytes by the ICSI-mediated
method. Transfer of 702 embryos produced by the ICSI-mediated method into five gilts resulted in 4 pregnancies. When three
of the recipients, which had received total 312 of the embryos were autopsied, 32 including 1 transgenic fetuses were obtained.
One of the recipients gave birth to three live piglets including one transgenic pig, showing a strong green fluorescence in
the eyeballs, oral mucous membrane and subcutaneous tissues. Fluorescent microscopy revealed uniform GFP expression in all
cell lines established from kidney, lung and muscle of the founder transgenic pig obtained. Nuclear transfer of these cells
resulted in stable in vitro development of cloned embryos into the blastocyst stage, ranging from 12.9 to 19.8%. When 767 of the nuclear transfer embryos
were transferred to 5 recipients, all became pregnant and gave birth to a total of six live transgenic-clones. The transgene
copy number and integrity in the founder pig were maintained in the primary culture cells established from the founder as
well as in the clones produced from these cells. Our study demonstrates that the ICSI-mediated gene transfer is an efficient
and practical method to produce transgenic pigs, using frozen sperm heads and in vitro matured oocytes. It was also shown that combination of ICSI-mediated transgenesis and nuclear transfer is a feasible technology
of great potential in transgenic pig production. 相似文献