Considerazioni sulla torulopsis pulcherrima

Zusammenfassung Aus Mosten von Umbrien und Toscana wurden 23 zu Torulopsis pulcherrima (Lindner) Saccardo gehörige Stämme isoliert, von denen 21 als die typische Art zu identifizieren waren (Rötung der Kultur nur bei Gegenwart von Eisen), während 2 Stämme, als var. rubra n. var. bezeichnet, sich außer durch andere Eigenschaften auch durch intensivere Farbstoffbildung unterscheiden.     

Ultrastructural complexity of nuclear components during early apoptotic phases in breast cancer cells.

Fractal morphometry was used to investigate the ultrastructural features of the plasma membrane, perinuclear membrane and nuclear chromatin in SK-BR-3 human breast cancer cells undergoing apoptosis. Cells were incubated with 1 microM calcimycin (A23187) for 24 h. Cells in the early stage of apoptosis had fractal dimension (FD) values indicating that their plasma membranes were less rough (lower FD) than those of control cells, while their perinuclear membranes were unaffected. Changes of the chromatin texture within the entire nucleus and in selected nuclear domains were more pronounced in treated cells. This confirms that the morphological reorganization imputable to a loss of structural complexity (reduced FD) occurs in the early stage of apoptosis, is accompanied by the inhibition of distinct enzymatic events and precedes the onset of conventional cellular markers, which can only be detected during the active phases of the apoptotic process.