排序方式: 共有196条查询结果,搜索用时 17 毫秒
61.
Hu H Eggers K Chen W Garshasbi M Motazacker MM Wrogemann K Kahrizi K Tzschach A Hosseini M Bahman I Hucho T Mühlenhoff M Gerardy-Schahn R Najmabadi H Ropers HH Kuss AW 《American journal of human genetics》2011,89(3):407-414
The genetic variants leading to impairment of intellectual performance are highly diverse and are still poorly understood. ST3GAL3 encodes the Golgi enzyme β-galactoside-α2,3-sialyltransferase-III that in humans predominantly forms the sialyl Lewis a epitope on proteins. ST3GAL3 resides on chromosome 1 within the MRT4 locus previously identified to associate with nonsyndromic autosomal recessive intellectual disability. We searched for the disease-causing mutations in the MRT4 family and a second independent consanguineous Iranian family by using a combination of chromosome sorting and next-generation sequencing. Two different missense changes in ST3GAL3 cosegregate with the disease but were absent in more than 1000 control chromosomes. In cellular and biochemical test systems, these mutations were shown to cause ER retention of the Golgi enzyme and drastically impair ST3Gal-III functionality. Our data provide conclusive evidence that glycotopes formed by ST3Gal-III are prerequisite for attaining and/or maintaining higher cognitive functions. 相似文献
62.
Babasafari Mahdieh Kourosharami Masoumeh Behman Jamie Farhadi Mona Komaki Alireza 《International journal of peptide research and therapeutics》2020,26(3):1485-1491
International Journal of Peptide Research and Therapeutics - Orexin-A well-known as hypocretin-1 is a neuropeptide that is produced in the lateral hypothalamus area (LHA). It promotes widespread... 相似文献
63.
Leila J Hamdan Richard B Coffin Masoumeh Sikaroodi Jens Greinert Tina Treude Patrick M Gillevet 《The ISME journal》2013,7(4):685-696
Prokaryote communities were investigated on the seasonally stratified Alaska Beaufort Shelf (ABS). Water and sediment directly underlying water with origin in the Arctic, Pacific or Atlantic oceans were analyzed by pyrosequencing and length heterogeneity-PCR in conjunction with physicochemical and geographic distance data to determine what features structure ABS microbiomes. Distinct bacterial communities were evident in all water masses. Alphaproteobacteria explained similarity in Arctic surface water and Pacific derived water. Deltaproteobacteria were abundant in Atlantic origin water and drove similarity among samples. Most archaeal sequences in water were related to unclassified marine Euryarchaeota. Sediment communities influenced by Pacific and Atlantic water were distinct from each other and pelagic communities. Firmicutes and Chloroflexi were abundant in sediment, although their distribution varied in Atlantic and Pacific influenced sites. Thermoprotei dominated archaea in Pacific influenced sediments and Methanomicrobia dominated in methane-containing Atlantic influenced sediments. Length heterogeneity-PCR data from this study were analyzed with data from methane-containing sediments in other regions. Pacific influenced ABS sediments clustered with Pacific sites from New Zealand and Chilean coastal margins. Atlantic influenced ABS sediments formed another distinct cluster. Density and salinity were significant structuring features on pelagic communities. Porosity co-varied with benthic community structure across sites and methane did not. This study indicates that the origin of water overlying sediments shapes benthic communities locally and globally and that hydrography exerts greater influence on microbial community structure than the availability of methane. 相似文献
64.
Objective:
Ghrelin, orexin, and galanin are orexigenic factors in rodents and humans which participate in adaptive response to weight loss. On the other hand, weight loss and fasting is accompanied by increased levels of epinephrine (Ep) and cortisol (Cor). In this study, we investigated the effects of Ep and Cor on fasting‐induced ghrelin, orexin, and galanin secretion in rats fed different levels of their energy requirements.Design:
Forty five male Wistar rats (300‐350 g, 15 per group) were fed a diet containing 100, 50, and 25% of their energy requirement for 10 days followed by 2 days of fasting. Animals were then anesthetized for carotid artery cannulation for injections and blood samplings.Methods:
Rats received either 3 µg Ep/kg body weight (BW), 3 µg Cor/kg BW, or a combination of those two (0.1 mg in 1 ml of phosphate‐buffered saline). Blood samples were collected before, 30, 60, and 120 min after injection.Results:
In normal and 50% food restricted groups, fasting ghrelin levels fell after Ep and combination of Ep and Cor injection (P ≤ 0.05), whereas, orexin were decreased by combination of Ep and Cor injection in rats fed 100% of their needs and Ep alone in rats fed 50%. Galanin just fell after combination of Ep and Cor injection in both starved (50%) and normal rats. In contrast, all groups whit 25% fed ad libitum did not response to any injections (P > 0.05).Conclusions:
These results indicate that Ep suppresses starvation‐induced secretion of ghrelin, orexin, and galanin in normal (100%) and starved (50%) rats and their response to Ep might be affected by weight loss. 相似文献65.
Hamdan LJ Gillevet PM Pohlman JW Sikaroodi M Greinert J Coffin RB 《FEMS microbiology ecology》2011,77(3):518-532
Sediments from the Porangahau ridge, located off the northeastern coast of New Zealand, were studied to describe bacterial community structure in conjunction with differing biogeochemical regimes across the ridge. Low diversity was observed in sediments from an eroded basin seaward of the ridge and the community was dominated by uncultured members of the Burkholderiales. Chloroflexi/GNS and Deltaproteobacteria were abundant in sediments from a methane seep located landward of the ridge. Gas-charged and organic-rich sediments further landward had the highest overall diversity. Surface sediments, with the exception of those from the basin, were dominated by Rhodobacterales sequences associated with organic matter deposition. Taxa related to the Desulfosarcina/Desulfococcus and the JS1 candidates were highly abundant at the sulfate-methane transition zone (SMTZ) at three sites. To determine how community structure was influenced by terrestrial, pelagic and in situ substrates, sequence data were statistically analyzed against geochemical data (e.g. sulfate, chloride, nitrogen, phosphorous, methane, bulk inorganic and organic carbon pools) using the Biota-Environmental matching procedure. Landward of the ridge, sulfate was among the most significant structuring factors. Seaward of the ridge, silica and ammonium were important structuring factors. Regardless of the transect location, methane was the principal structuring factor on SMTZ communities. 相似文献
66.
Ghaderi M Allameh A Soleimani M Rastegar H Ahmadi-Ashtiani HR 《Mutation research》2011,719(1-2):14-20
This study compared the sensitivity of differentiated hepatocyte-like cells, their progenitor mesenchymal stem cells (MSCs) and CD34(+) stem cells to DNA damage and toxicity induced by aflatoxin B1 (AFB1). The hepatocyte-like cells and their progenitor cells (isolated from umbilical cord blood (UCB)) were each treated with AFB1 on day 15 of differentiation. Cell toxicity and genotoxicity effects were assessed using MTT and alkaline comet assays. AFB1 treatment resulted in a dose- and time-dependent inhibition of cell growth. The IC(50) values of AFB1 for hepatocytes differentiated from CD34(+) and MSCs were within the same range (44.7-46.8μM). The IC(50) calculated for non-differentiated MSCs and CD34(+) cells was slightly lower (42.0-43.4μM) than that calculated for their differentiated counterparts. However, the extent of DNA damage was different in differentiated and non-differentiated cells. The percentages of DNA (% DNA) in comet tails measured in hepatocytes differentiated from MSCs exposed to AFB1 (0, 2.5, 10 and 20μM) for 24h were ~15, 55, 65 and 70%, respectively. In comparison, hepatocytes from CD34(+) cells were more resistant to AFB1-induced DNA damage. Hepatocyte-MSCs were most sensitive to DNA damage, followed by UCB-CD34(+) cells, then UCB-MSCs and finally hepatocyte-CD34(+) cells. These results clearly showed that stem cells from different sources have different sensitivities to DNA damaging agents. These differences can be assigned to the expression levels of cytochrome P450 (CYP) particularly CYP3A4 in non-differentiated and differentiated cells. These data are useful in better understanding the susceptibility/resistance of stem cells in the process of differentiation to environmental toxicants. 相似文献
67.
Vaezi Mohammad Amin Eghtedari Amir Reza Safizadeh Banafsheh Ghasempour Ghasem Salimi Vahid Nourbakhsh Mitra Nazem Shima Tavakoli-Yaraki Masoumeh 《Molecular biology reports》2022,49(10):9409-9427
Molecular Biology Reports - Understanding the molecular mechanism underlying the pathophysiology of primary skeletal tumors is crucial due to the tumor-related complications, incidence at... 相似文献
68.
Hashemi Mohammad Sadeghi Abbas Saghi Masoumeh Aminzare Majid Raeisi Mojtaba Rezayi Majid Sany Seyedeh Belin Tavakoly 《Biological trace element research》2019,188(2):485-493
Biological Trace Element Research - The relative contribution of foodstuffs to intake of heavy metal is still equivocal, and thus, available data are rare. Here, the concentration of ten heavy... 相似文献
69.
Nicolas Delpierre Sgolne Lireux Florian Hartig Jesus Julio Camarero Alissar Cheaib Katarina ufar Henri Cuny Annie Deslauriers Patrick Fonti Joica Gri
ar Jian‐Guo Huang Cornelia Krause Guohua Liu Martin de Luis Harri Mkinen Edurne Martinez del Castillo Hubert Morin Pekka Njd Walter Oberhuber Peter Prislan Sergio Rossi Seyedeh Masoumeh Saderi Vaclav Treml Hanus Vavrick Cyrille B. K. Rathgeber 《Global Change Biology》2019,25(3):1089-1105
The phenology of wood formation is a critical process to consider for predicting how trees from the temperate and boreal zones may react to climate change. Compared to leaf phenology, however, the determinism of wood phenology is still poorly known. Here, we compared for the first time three alternative ecophysiological model classes (threshold models, heat‐sum models and chilling‐influenced heat‐sum models) and an empirical model in their ability to predict the starting date of xylem cell enlargement in spring, for four major Northern Hemisphere conifers (Larix decidua, Pinus sylvestris, Picea abies and Picea mariana). We fitted models with Bayesian inference to wood phenological data collected for 220 site‐years over Europe and Canada. The chilling‐influenced heat‐sum model received most support for all the four studied species, predicting validation data with a 7.7‐day error, which is within one day of the observed data resolution. We conclude that both chilling and forcing temperatures determine the onset of wood formation in Northern Hemisphere conifers. Importantly, the chilling‐influenced heat‐sum model showed virtually no spatial bias whichever the species, despite the large environmental gradients considered. This suggests that the spring onset of wood formation is far less affected by local adaptation than by environmentally driven plasticity. In a context of climate change, we therefore expect rising winter–spring temperature to exert ambivalent effects on the spring onset of wood formation, tending to hasten it through the accumulation of forcing temperature, but imposing a higher forcing temperature requirement through the lower accumulation of chilling. 相似文献
70.
Kloosterman WP Tavakoli-Yaraki M van Roosmalen MJ van Binsbergen E Renkens I Duran K Ballarati L Vergult S Giardino D Hansson K Ruivenkamp CA Jager M van Haeringen A Ippel EF Haaf T Passarge E Hochstenbach R Menten B Larizza L Guryev V Poot M Cuppen E 《Cell reports》2012,1(6):648-655
Highlights? Constitutional complex chromosomal rearrangements display unanticipated complexity resembling chromothripsis ? Some chromothripsis rearrangements involve clustered double-stranded DNA breaks ? There exist distinct classes of chromothripsis rearrangements 相似文献