全文获取类型
收费全文 | 3410篇 |
免费 | 185篇 |
国内免费 | 4篇 |
专业分类
3599篇 |
出版年
2023年 | 13篇 |
2022年 | 30篇 |
2021年 | 53篇 |
2020年 | 33篇 |
2019年 | 39篇 |
2018年 | 46篇 |
2017年 | 33篇 |
2016年 | 72篇 |
2015年 | 103篇 |
2014年 | 130篇 |
2013年 | 206篇 |
2012年 | 192篇 |
2011年 | 221篇 |
2010年 | 128篇 |
2009年 | 129篇 |
2008年 | 193篇 |
2007年 | 188篇 |
2006年 | 191篇 |
2005年 | 196篇 |
2004年 | 219篇 |
2003年 | 193篇 |
2002年 | 188篇 |
2001年 | 62篇 |
2000年 | 53篇 |
1999年 | 62篇 |
1998年 | 42篇 |
1997年 | 38篇 |
1996年 | 25篇 |
1995年 | 24篇 |
1994年 | 20篇 |
1993年 | 27篇 |
1992年 | 41篇 |
1991年 | 45篇 |
1990年 | 33篇 |
1989年 | 30篇 |
1988年 | 32篇 |
1987年 | 27篇 |
1986年 | 28篇 |
1985年 | 22篇 |
1984年 | 18篇 |
1983年 | 16篇 |
1982年 | 21篇 |
1981年 | 13篇 |
1980年 | 10篇 |
1979年 | 20篇 |
1978年 | 9篇 |
1977年 | 8篇 |
1976年 | 13篇 |
1971年 | 12篇 |
1969年 | 10篇 |
排序方式: 共有3599条查询结果,搜索用时 15 毫秒
941.
Preventive effects of Cladosiphon fucoidan against Helicobacter pylori infection in Mongolian gerbils 总被引:6,自引:0,他引:6
Shibata H Iimuro M Uchiya N Kawamori T Nagaoka M Ueyama S Hashimoto S Yokokura T Sugimura T Wakabayashi K 《Helicobacter》2003,8(1):59-65
Background. Recently, the acquisition by Helicobacter pylori of resistance to antibiotics has become a serious problem. Therefore, nonantibiotic substances are required to diminish H. pylori‐induced gastric lesions. In the present study, the effects of Cladosiphon fucoidan were examined in terms of H. pylori attachment to porcine gastric mucin in vitro and Helicobacter pylori‐induced gastritis in vivo. Methods. The inhibitory effect of Cladosiphon fucoidan and other polysaccharides on H. pylori attachment to porcine gastric mucin was assayed in vitro with mucin‐coated microtiter plates. The effect of Cladosiphon fucoidan on H. pylori‐induced gastritis was examined in vivo using Mongolian gerbils. H. pylori‐inoculated gerbils were given fucoidan in drinking water. Six weeks after H. pylori‐inoculation, gerbils were sacrificed for macroscopic and microscopic examination of gastric lesions and counting of viable H. pylori in the gastric mucosa. Results. Cladosiphon fucoidan inhibited the H. pylori attachment to porcine gastric mucin at pH 2.0 and 4.0. Two other sulfated polysaccharides, Fucus fucoidan and dextran sulfate sodium, also inhibited the attachment but only at pH 2.0. Inhibitory effects of these three sulfated polysaccharides were not observed at pH 7.2 and nonsulfated polysaccharides, such as mannan and dextran, exerted no influence at any pH. In the in vivo experiment, the H. pylori‐induced gastritis and the prevalence of H. pylori infected animals were markedly reduced by fucoidan in a dose‐dependent manner, at doses of 0.05 and 0.5% in the drinking water. Conclusion. Cladosiphon fucoidan may deserve particular attention as a safe agent that can prevent H. pylori infection and reduce the risk of associated gastric cancer. 相似文献
942.
Tserenpurev Bat-Oyun Masato Shinoda Yunxiang Cheng Yadamjav Purevdorj 《Journal of Plant Ecology》2016,9(5):508
Aims Grazing and water availability are the primary drivers of vegetation dynamics in grazing-dominated regions of Mongolia with a semi-arid climate and frequent droughts. Nomadic animal husbandry still plays a large part in the economy of Mongolia, but more variable precipitation regime and increase in livestock number have severely affected grassland ecosystems through overgrazing, leading to pasture degradation. This study aimed to examine the effects of grazing exclusion, interannual variation of plant-available precipitation (PAP) and their interaction on the aboveground biomass (AGB) of each dominant species, the AGB of annual species and the total AGB in a Mongolian dry steppe, using long-term field data.Methods To detect the effect of grazing on vegetation dynamics, vegetation surveys were conducted in a non-grazed exclosure zone and a fully grazed area outside the exclosure. We assessed the effects of grazing, PAP and their interaction on AGB parameters using a generalized linear model. A detrended correspondence analysis (DCA) was used to visualize the effects of grazing and PAP on the AGB of each species.Important findings Grazing, PAP and their interaction had significant effects on AGB. The effect of grazing on AGB was larger with higher precipitation and higher amounts of AGB (i.e. forage) while AGB was strongly limited in drought years, which resulted in a smaller grazing effect. The current year PAP had the highest impact (r = 0.88, P < 0.01) on AGB. The dominance of annual species was characterized by the amount of PAP in the current and preceding years: annuals dominated in wet years that followed consecutive dry years. The DCA Axis 1 reflected the variation of AGB with interannual variation of PAP while the DCA Axis 2 differentiated the grazing effect. The DCA scatter diagram based on species score illustrated that Artemisia adamsii (an unpalatable herb) was clearly linked to grazing disturbance whereas palatable perennials such as Agropyron cristatum, Stipa krylovii and Cleistogenes squarrosa were related to grazing abandonment and wetter conditions. In brief, number of livestock, hence the grazing impacts on vegetation dynamic in this region could have driven by forage availability, which is mainly controlled by current-year PAP. 相似文献
943.
Ishii A Nonaka T Taniguchi S Saito T Arai T Mann D Iwatsubo T Hisanaga S Goedert M Hasegawa M 《FEBS letters》2007,581(24):4711-4717
In Lewy body diseases and multiple system atrophy, α-synuclein is hyperphosphorylated at Ser129, suggesting a role in pathogenesis. Here, we report purification of the protein kinase in rat brain that phosphorylates Ser129 and its identification as casein kinase-2 (CK2). We show that most of the activity can be inhibited by heparin, an inhibitor of CK2. Phosphorylated Ser129 was detected in primary cultured neurons and inhibited by CK2 inhibitors. In some cases of Lewy body disease, CK2-like immunoreactivity was recovered in the sarkosyl-insoluble fraction, which was enriched in phosphorylated α-synuclein. Taken together, these findings suggest that CK2 may be involved in the hyperphosphorylation of α-synuclein in α-synucleinopathies. 相似文献
944.
945.
Growing evidence suggests that temporally fluctuating environments are important in maintaining variation both within and between species. To date, however, studies of genetic variation within a population have been largely conducted by evolutionary biologists (particularly population geneticists), while population and community ecologists have concentrated more on diversity at the species level. Despite considerable conceptual overlap, the commonalities and differences of these two alternative paradigms have yet to come under close scrutiny. Here, we review theoretical and empirical studies in population genetics and community ecology focusing on the ‘temporal storage effect’ and synthesise theories of diversity maintenance across different levels of biological organisation. Drawing on Chesson's coexistence theory, we explain how temporally fluctuating environments promote the maintenance of genetic variation and species diversity. We propose a further synthesis of the two disciplines by comparing models employing traditional frequency-dependent dynamics and those adopting density-dependent dynamics. We then address how temporal fluctuations promote genetic and species diversity simultaneously via rapid evolution and eco-evolutionary dynamics. Comparing and synthesising ecological and evolutionary approaches will accelerate our understanding of diversity maintenance in nature. 相似文献
946.
The early development and occurrence patterns of Argyrops bleekeri are described based on 87 specimens collected from Nakagusuku Bay on Okinawa Island in southwestern Japan. Larvae and juveniles of the genus Argyrops are distinguished from the other seabreams inhabiting the Western Pacific region by the strength and extent of head spination, body depth, dorsal-fin-ray counts and melanophore patterns. Argyrops bleekeri is easily distinguished from other members of this genus by the presence of a single rudimentary dorsal-fin spine on the first dorsal pterygiophore, melanophore patterns and an allopatric distribution. Argyrops bleekeri larvae [3.3–7.1 mm body length (BL)] and juveniles (6.7–13.0 mm BL) were found in the bay from January to May; nonetheless, they were not collected from the outer bay or in extremely shallow inshore areas such as tidal flats. The results suggest that Argyrops is the most derived red seabream because of its spiny morphology, and it may be a member of an expanding nearshore group of red seabreams, which originally inhabited offshore waters. 相似文献
947.
Systematic Parasitology - Three species of Dioncopseudobenedenia Yamaguti, 1965 (Monogenea: Capsalidae: Benedeniinae) have been described, all found parasitising Naso spp. (Acanthuridae) and... 相似文献
948.
Masato Mizuno Shuhei Ebine Okuto Shounai Shizuka Nakajima Shota Tomomatsu Ken Ikeuchi Yoshitaka Matsuo Toshifumi Inada 《Nucleic acids research》2021,49(4):2102
Ribosome stalling at tandem CGA codons or poly(A) sequences activates quality controls for nascent polypeptides including ribosome-associated quality control (RQC) and no-go mRNA decay (NGD). In RQC pathway, Hel2-dependent uS10 ubiquitination and the RQC-trigger (RQT) complex are essential for subunit dissociation, and Ltn1-dependent ubiquitination of peptidyl-tRNA in the 60S subunit requires Rqc2. Here, we report that polytryptophan sequences induce Rqc2-independent RQC. More than 11 consecutive tryptophan residues induced RQC in a manner dependent on Hel2-mediated ribosome ubiquitination and the RQT complex. Polytryptophan sequence-mediated RQC was not coupled with CAT-tailing, and Rqc2 was not required for Ltn1-dependent degradation of the arrest products. Eight consecutive tryptophan residues located at the region proximal to the peptidyl transferase center in the ribosome tunnel inhibited CAT-tailing by tandem CGA codons. Polytryptophan sequences also induced Hel2-mediated canonical RQC-coupled NGD and RQC-uncoupled NGD outside the stalled ribosomes. We propose that poly-tryptophan sequences induce Rqc2-independent RQC, suggesting that CAT-tailing in the 60S subunit could be modulated by the polypeptide in the ribosome exit tunnel. 相似文献
949.
950.
Particles of mung bean yellow mosaic virus (MYMV) were purified by a method that yields up to 3 mg per kg of systemically infected Phaseolus vulgaris“Top Crop” and used to prepare antiserum. MYMV antiserum prepared gave a single precipitin line and had a titre of 1/512 with homologous virus in gel double-diffusion tests. MYMV was shown to be serologically related to other whitefly-transmitted viruses, bean golden mosaic virus, tobacco leaf curl virus and cassava latent virus. 相似文献