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101.
Carla Martins Lopes Marta De Barba Frdric Boyer Cline Mercier Daniel Galiano Bruno Busnello Kubiak Renan Maestri Pedro Joel Silva da Silva Filho Ludovic Gielly Eric Coissac Thales Renato Ochotorena de Freitas Pierre Taberlet 《Molecular ecology》2020,29(16):3143-3153
Knowledge of how animal species use food resources available in the environment can increase our understanding of many ecological processes. However, obtaining this information using traditional methods is difficult for species feeding on a large variety of food items in highly diverse environments. We amplified the DNA of plants for 306 scat and 40 soil samples, and applied an environmental DNA metabarcoding approach to investigate food preferences, degree of diet specialization and diet overlap of seven herbivore rodent species of the genus Ctenomys distributed in southern and midwestern Brazil. The metabarcoding approach revealed that these species consume more than 60% of the plant families recovered in soil samples, indicating generalist feeding habits of ctenomyids. The family Poaceae was the most common food resource retrieved in scats of all species as well in soil samples. Niche overlap analysis indicated high overlap in the plant families and molecular operational taxonomic units consumed, mainly among the southern species. Interspecific differences in diet composition were influenced, among other factors, by the availability of resources in the environment. In addition, our results provide support for the hypothesis that the allopatric distributions of ctenomyids allow them to exploit the same range of resources when available, possibly because of the absence of interspecific competition. 相似文献
102.
Livia Pilatti PhD Renato Mancini Astray Mayra Pereira Rocca Flavia Ferreira Barbosa Soraia Attie Calil Jorge Michael Butler Elisabeth de Fátima Pires Augusto 《Biotechnology progress》2020,36(6):e3046
Most rabies vaccines are based on inactivated virus, which production process demands a high level of biosafety structures. In the past decades, recombinant rabies virus glycoprotein (RVGP) produced in several expression systems has been extensively studied to be used as an alternative vaccine. The immunogenic characteristics of this protein depend on its correct conformation, which is present only after the correct post-translational modifications, typically performed by animal cells. The main challenge of using this protein as a vaccine candidate is to keep its trimeric conformation after the purification process. We describe here a new immunoaffinity chromatography method using a monoclonal antibody for RVGP Site II for purification of recombinant rabies virus glycoprotein expressed on the membrane of Drosophila melanogaster S2 cells. RVGP recovery achieved at least 93%, and characterization analysis showed that the main antigenic proprieties were preserved after purification. 相似文献
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Emilia Trudnowska Kaja Balazy Joanna StoEgiert Irina Smolina Thomas Brown Marta Gluchowska 《Ecology and evolution》2020,10(24):14067
Copepods of the genus Calanus are the key components of zooplankton. Understanding their response to a changing climate is crucial to predict the functioning of future warmer high‐latitude ecosystems. Although specific Calanus species are morphologically very similar, they have different life strategies and roles in ecosystems. In this study, C. finmarchicus and C. glacialis were thoroughly studied with regard to their plasticity in morphology and ecology both in their preferred original water mass (Atlantic vs. Arctic side of the Polar Front) and in suboptimal conditions (due to, e.g., temperature, turbidity, and competition in Hornsund fjord). Our observations show that “at the same place and time,” both species can reach different sizes, take on different pigmentation, be in different states of population development, utilize different reproductive versus lipid accumulation strategies, and thrive on different foods. Size was proven to be a very mutable morphological trait, especially with regard to reduced length of C. glacialis. Both species exhibited pronounced red pigmentation when inhabiting their preferred water mass. In other domains, C. finmarchicus individuals tended to be paler than C. glacialis individuals. Gonad maturation and population development indicated mixed reproductive strategies, although a surprisingly similar population age structure of the two co‐occurring species in the fjord was observed. Lipid accumulation was high and not species‐specific, and its variability was due to diet differences of the populations. According to the stable isotope composition, both species had a more herbivorous diatom‐based diet in their original water masses. While the diet of C. glacialis was rather consistent among the domains studied, C. finmarchicus exhibited much higher variability in its feeding history (based on lipid composition). Our results show that the plasticity of both Calanus species is indeed impressive and may be regulated differently, depending on whether they live in their “comfort zone” or beyond it. 相似文献
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106.
Gogliettino Marta Cocca Ennio Sandomenico Annamaria Gratino Lorena Iaccarino Emanuela Calvanese Luisa Rossi Mosè Palmieri Gianna 《Molecular biology reports》2021,48(2):1505-1519
Molecular Biology Reports - Serine hydrolases play crucial roles in many physiological and pathophysiological processes and a panel of these enzymes are targets of approved drugs. Despite this,... 相似文献
107.
Jankowska Urszula Skupien-Rabian Bozena Swiderska Bianka Prus Gabriela Dziedzicka-Wasylewska Marta Kedracka-Krok Sylwia 《Neurochemical research》2021,46(8):2097-2111
Neurochemical Research - Although antipsychotics are routinely used in the treatment of schizophrenia for the last decades, their precise mechanism of action is still unclear. In this study, we... 相似文献
108.
de Sousa Sylvia Morais de Oliveira Christiane Abreu Andrade Daniele Luiz de Carvalho Chainheny Gomes Ribeiro Vitória Palhares Pastina Maria Marta Marriel Ivanildo Evódio de Paula Lana Ubiraci Gomes Gomes Eliane Aparecida 《Journal of Plant Growth Regulation》2021,40(2):867-877
Journal of Plant Growth Regulation - The rising demand for agricultural commodities in developing countries has put increasing pressure on land resources for higher yields, with associated growth... 相似文献
109.