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941.
L.?R.?BertoliniEmail author H.?Meade C.?R.?Lazzarotto L.?T.?Martins K.?C.?Tavares M.?Bertolini J.?D.?Murray 《Transgenic research》2016,25(3):329-343
The recombinant production of therapeutic proteins for human diseases is currently the largest source of innovation in the pharmaceutical industry. The market growth has been the driving force on efforts for the development of new therapeutic proteins, in which transgenesis emerges as key component. The use of the transgenic animal platform offers attractive possibilities, residing on the low production costs allied to high productivity and quality of the recombinant proteins. Although many strategies have evolved over the past decades for the generation of transgenic founders, transgenesis in livestock animals generally faces some challenges, mainly due to random transgene integration and control over transgene copy number. But new developments in gene editing with CRISPR/Cas system promises to revolutionize the field for its simplicity and high efficiency. In addition, for the final approval of any given recombinant protein for animal or human use, the production and characterization of bioreactor founders and expression patterns and functionality of the proteins are technical part of the process, which also requires regulatory and administrative decisions, with a large emphasis on biosafety. The approval of two mammary gland-derived recombinant proteins for commercial and clinical use has boosted the interest for more efficient, safer and economic ways to generate transgenic founders to meet the increasing demand for biomedical proteins worldwide. 相似文献
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Maria Silva Cunha Marcelo Felgueiras Napoli 《Studies on Neotropical Fauna and Environment》2016,51(2):144-151
We studied bromeliad selection by calling males of Phyllodytes melanomystax. The study site was a restinga environment in the northeastern state of Bahia, northeastern Brazil. We sampled 202 bromeliads, 101 with and 101 without calling males. We used multiple logistic regression analysis and Wald test to identify which of nine environmental variables investigated could explain the occurrence of calling males within bromeliads. The presence/absence of calling males in bromeliads was influenced by the number of bromeliads in a 2 m radius and the amount of debris inside the rosettes, while physical variables of bromeliads and the volume of stored water inside their rosettes had no influence. The mark-recapture procedure of P. melanomystax revealed site fidelity. This study is the first to explain the pattern of bromeliad selection by a species of the bromeliad-dwelling frog genus Phyllodytes. 相似文献
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Claudia Etchebehere Elena Castelló Jorge Wenzel Mélida del Pilar Anzola-Rojas Liliana Borzacconi Germán Buitrón Lea Cabrol Vivian María Carminato Julian Carrillo-Reyes Crhistian Cisneros-Pérez Laura Fuentes Iván Moreno-Andrade Elías Razo-Flores Gonzalo Ruiz Filippi Estela Tapia-Venegas Javiera Toledo-Alarcón Marcelo Zaiat 《Applied microbiology and biotechnology》2016,100(7):3371-3384
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