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101.
The effect of the inoculum source on the digestion of linear alkylbenzene sulfonates (LAS) under anaerobic conditions has
been investigated. The potential for primary and ultimate LAS biodegradation of anaerobic sludge samples obtained from wastewater
treatment plants (WWTPs) of different geographical locations was studied applying a batch test system. It was found that only
4–22% of the LAS added to the batch anaerobic digesters was primarily transformed suggesting a poor primary degradation of
the LAS molecule in anaerobic discontinuous systems. Regarding ultimate biodegradation, the addition of LAS to the batch anaerobic
digesters caused a reduction on the extent of biogas production. Significant differences in the inhibition extent of the biogas
production were observed (4–26%) depending on the sludge used as inoculum. Effect of the surfactant on the anaerobic microorganisms
was correlated with its concentration in the aqueous phase. Sorption of LAS on anaerobic sludge affects its toxicity by depletion
of the available fraction of the surfactant. LAS content on sludge was related to the total amount of calcium and magnesium
extractable ions. The presence of divalent cations promote the association of LAS with anaerobic sludge reducing its bioavailability
and the extent of its inhibitory effect on the biogas production. 相似文献
102.
Esteve Fernández Marcela Fu José A. Pascual María J. López Mónica Pérez-Ríos Anna Schiaffino Jose M. Martínez-Sánchez Carles Ariza Esteve Saltó Manel Nebot and the Spanish Smoking Law Evaluation Group 《PloS one》2009,4(1)
Background
A smoke-free law came into effect in Spain on 1st January 2006, affecting all enclosed workplaces except hospitality venues, whose proprietors can choose among totally a smoke-free policy, a partial restriction with designated smoking areas, or no restriction on smoking on the premises. We aimed to evaluate the impact of the law among hospitality workers by assessing second-hand smoke (SHS) exposure and the frequency of respiratory symptoms before and one year after the ban.Methods and Finding
We formed a baseline cohort of 431 hospitality workers in Spain and 45 workers in Portugal and Andorra. Of them, 318 (66.8%) were successfully followed up 12 months after the ban, and 137 nonsmokers were included in this analysis. We obtained self-reported exposure to SHS and the presence of respiratory symptoms, and collected saliva samples for cotinine measurement. Salivary cotinine decreased by 55.6% after the ban among nonsmoker workers in venues where smoking was totally prohibited (from median of 1.6 ng/ml before to 0.5 ng/ml, p<0.01). Cotinine concentration decreased by 27.6% (p = 0.068) among workers in venues with designated smoking areas, and by 10.7% (p = 0.475) among workers in venues where smoking was allowed. In Portugal and Andorra, no differences between cotinine concentration were found before (1.2 ng/ml) and after the ban (1.2 ng/ml). In Spain, reported respiratory symptom declined significantly (by 71.9%; p<0.05) among workers in venues that became smoke-free. After adjustment for potential confounders, salivary cotinine and respiratory symptoms decreased significantly among workers in Spanish hospitality venues where smoking was totally banned.Conclusions
Among nonsmoker hospitality workers in bars and restaurants where smoking was allowed, exposure to SHS after the ban remained similar to pre-law levels. The partial restrictions on smoking in Spanish hospitality venues do not sufficiently protect hospitality workers against SHS or its consequences for respiratory health. 相似文献103.
Landscape genetics is the amalgamation of landscape ecology and population genetics to help with understanding microevolutionary processes such as gene flow and adaptation. In this review, we examine why landscape genetics of plants lags behind that of animals, both in number of studies and consideration of landscape elements. The classical landscape distance/resistance approach to study gene flow is challenging in plants, whereas boundary detection and the assessment of contemporary gene flow are more feasible. By contrast, the new field of landscape genetics of adaptive genetic variation, establishing the relationship between adaptive genomic regions and environmental factors in natural populations, is prominent in plant studies. Landscape genetics is ideally suited to study processes such as migration and adaptation under global change. 相似文献
104.
Miguel Alcaide Juan J. Negro David Serrano José L. Antolín Sara Casado Manel Pomarol 《Conservation Genetics》2010,11(1):331-338
We used microsatellites to assess ongoing captive breeding and reintroduction programs of the lesser kestrel. The extent of
genetic variation within the captive populations analysed did not differ significantly from that reported in wild populations.
Thus, the application of widely recommended management practices, such as the registration of crosses between individuals
in proper stud books and the introduction of new individuals into the genetic pools, has proven satisfactory to maintain high
levels of genetic variation. The high rates of hatching failure occasionally documented in captivity can therefore not be
attributed to depressed genetic variation. Even though genetic diversity in reintroduced populations did not differ significantly
when compared to wild populations either, average observed heterozygosities and inbreeding coefficients were significantly
lower and higher, respectively, when compared to the captive demes where released birds came. Monitoring of reproductive parameters
during single-pairing breeding and paternity inference within colonial facilities revealed large variations in breeding success
between reproductive adults. The relative number of breeding pairs that contributed to a large part of captive-born birds
(50–56%) was similar in both cases (28.6 and 26.9%, respectively). Thus, the chances for polygyny (rarely in this study),
extra-pair paternity (not found in this study) and/or social stimulation of breeding parameters do not seem to greatly affect
the genetically effective population size. Independently of breeding strategies, the release of unrelated fledglings into
the same area and the promotion of immigration should be mandatory to counteract founder effects and avoid inbreeding in reintroduced
populations of lesser kestrels. 相似文献
105.
Gernot Segelbacher Samuel A. Cushman Bryan K. Epperson Marie-Josée Fortin Olivier Francois Olivier J. Hardy Rolf Holderegger Pierre Taberlet Lisette P. Waits Stéphanie Manel 《Conservation Genetics》2010,11(2):375-385
Landscape genetics plays an increasingly important role in the management and conservation of species. Here, we highlight
some of the opportunities and challenges in using landscape genetic approaches in conservation biology. We first discuss challenges
related to sampling design and introduce several recent methodological developments in landscape genetics (analyses based
on pairwise relatedness, the application of Bayesian methods, inference from landscape resistance and a shift from population-based
to individual-based analyses). We then show how simulations can foster the field of landscape genetics and, finally, elaborate
on technical developments in sequencing techniques that will dramatically improve our ability to study genetic variation in
wild species, opening up new and unprecedented avenues for genetic analysis in conservation biology. 相似文献
106.
Ana Garcia-Alvarez Marta Sitges María-Jesús Pinazo Ander Regueiro-Cueva Elizabeth Posada Silvia Poyatos José Tomás Ortiz-Pérez Magda Heras Manel Azqueta Joaquim Gascon Ginés Sanz 《PLoS neglected tropical diseases》2010,4(9)
Introduction
Chagas disease remains a major cause of mortality in several countries of Latin America and has become a potential public health problem in non-endemic countries as a result of migration flows. Cardiac involvement represents the main cause of mortality, but its diagnosis is still based on nonspecific criteria with poor sensitivity. Early identification of patients with cardiac involvement is desirable, since early treatment may improve prognosis. This study aimed to assess the role of diastolic dysfunction, abnormal myocardial strain and elevated brain natriuretic peptide (BNP) in the early identification of cardiac involvement in Chagas disease.Methodology/Principal Findings
Fifty-four patients divided into 3 groups—group 1 (undetermined form: positive serology without ECG or 2D-echocardiographic abnormalities; N = 32), group 2 (typical ECG abnormalities of Chagas disease but normal 2D-echocardiography; N = 14), and group 3 (regional wall motion abnormalities, left ventricular [LV] end-diastolic diameter >55 mm or LV ejection fraction <50% on echocardiography; N = 8)—and 44 control subjects were studied. Patients with significant non-cardiac diseases, other heart diseases and previous treatment with benznidazol were excluded. The median age was 37 (20–58) years; 40% were men. BNP levels, longitudinal and radial myocardial strain and LV diastolic dysfunction increased progressively from group 1 to 3 (p for trend <0.01). Abnormal BNP levels (>37 pg/ml) were noted in 0%, 13%, 29% and 63% in controls and groups 1 to 3, respectively. Half of patients in the undetermined form had impaired relaxation patterns, whereas half of patients with ECG abnormalities suggestive of Chagas cardiomyopathy had normal diastolic function. In group 1, BNP levels were statistically higher in patients with diastolic dysfunction as compared to those with normal diastolic function (27±26 vs. 11±8 pg/ml, p = 0.03).Conclusion/Significance
In conclusion, the combination of diastolic function and BNP measurement adds important information that could help to better stratify patients with Chagas disease. 相似文献107.
Here, we describe a role for mammalian DNA methyltransferases (DNMTs) in telomere length control. Mouse embryonic stem (ES) cells genetically deficient for DNMT1, or both DNMT3a and DNMT3b have dramatically elongated telomeres compared with wild-type controls. Mammalian telomere repeats (TTAGGG) lack the canonical CpG methylation site. However, we demonstrate that mouse subtelomeric regions are heavily methylated, and that this modification is decreased in DNMT-deficient cells. We show that other heterochromatic marks, such as histone 3 Lys 9 (H3K9) and histone 4 Lys 20 (H4K20) trimethylation, remain at both subtelomeric and telomeric regions in these cells. Lack of DNMTs also resulted in increased telomeric recombination as indicated by sister-chromatid exchanges involving telomeric sequences, and by the presence of 'alternative lengthening of telomeres' (ALT)-associated promyelocytic leukaemia (PML) bodies (APBs). This increased telomeric recombination may lead to telomere-length changes, although our results do not exclude a potential involvement of telomerase and telomere-binding proteins in the aberrant telomere elongation observed in DNMT-deficient cells. Together, these results demonstrate a previously unappreciated role for DNA methylation in maintaining telomere integrity. 相似文献
108.
Carme Cceres Enric Ribes Sylviane Muller Lluís Cornudella Manel Chiva 《Molecular reproduction and development》1994,38(4):440-452
During the process of chromatin cndensation in the spermiogenesis of the neogastropod mollusc Murex brandaris, the nuclear protein complement undergoes a complex series of changes. These changes lead to the appearance of three small protamines in the ripe sperm nuclei. We have characterized this system electrophoretically and at the compositions with antibodies elicited against a specific spermatozoan protamine. Our results indicate that the complex pattern of chromatin condensation during spermiogenesis in this species (M. brandaris) may be modulated by a series of post-translational (and intranuclear) modifications of DNA-interacting proteins, such as precursors to the sperm protamines. The amino acid composition of each sperm protamine is remarkably simple (lys + arg + gly ≥96 mol%). This system of spermiogenic/spermatozoal proteins in the neogastropod M. brandaris clearly differs from that in patellogastropods and archaeogastropods, and it may be helpful in understanding evolutionary changes in the chromatin condensation pattern during the spermiogenesis of gastropod molluscs. © 1994 Wiley-Liss, Inc. 相似文献
109.
DNA methylation polymorphisms precede any histological sign of atherosclerosis in mice lacking apolipoprotein E 总被引:11,自引:0,他引:11
Lund G Andersson L Lauria M Lindholm M Fraga MF Villar-Garea A Ballestar E Esteller M Zaina S 《The Journal of biological chemistry》2004,279(28):29147-29154
The present work investigates the occurrence and significance of aberrant DNA methylation patterns during early stages of atherosclerosis. To this end, we asked whether the genetically atherosclerosis-prone APOE-null mice show any changes in DNA methylation patterns before the appearance of histologically detectable vascular lesion. We exploited a combination of various techniques: DNA fingerprinting, in vitro methyl-accepting assay, 5-methylcytosine quantitation, histone post-translational modification analysis, Southern blotting, and PCR. Our results show that alterations in DNA methylation profiles, including both hyper- and hypomethylation, were present in aortas and PBMC of 4-week-old mutant mice with no detectable atherosclerotic lesion. Sequencing and expression analysis of 60 leukocytic polymorphisms revealed that epigenetic changes involve transcribed genic sequences, as well as repeated interspersed elements. Furthermore, we showed for the first time that atherogenic lipoproteins promote global DNA hypermethylation in a human monocyte cell line. Taken together, our results unequivocally show that alterations in DNA methylation profiles are early markers of atherosclerosis in a mouse model and may play a causative role in atherogenesis. 相似文献
110.
Chemokines determine local lymphoneogenesis and a reduction of circulating CXCR4+ T and CCR7 B and T lymphocytes in thyroid autoimmune diseases 总被引:8,自引:0,他引:8
Armengol MP Cardoso-Schmidt CB Fernández M Ferrer X Pujol-Borrell R Juan M 《Journal of immunology (Baltimore, Md. : 1950)》2003,170(12):6320-6328
Chemokines and their corresponding receptors are crucial for the recruitment of lymphocytes into the lymphoid organs and for its organization acting in a multistep process. Tissues affected by autoimmune disease often contain ectopic lymphoid follicles which, in the case of autoimmune thyroid disorders, are highly active and specific for thyroid Ags although its pathogenic role remains unclear. To understand the genesis of these lymphoid follicles, the expression of relevant cytokines and chemokines was assessed by real time PCR, immunohistochemistry and by in vitro assays in autoimmune and nonautoimmune thyroid glands. Lymphotoxin alpha, lymphotoxin beta, C-C chemokine ligand (CCL) 21, CXC chemokine ligand (CXCL) 12, CXCL13, and CCL22 were increased in thyroids from autoimmune patients, whereas CXCL12, CXCL13, and CCL22 levels were significantly higher in autoimmune glands with ectopic secondary lymphoid follicles than in those without follicles. Interestingly, thyroid epithelium produced CXCL12 in response to proinflammatory cytokines providing a possible clue for the understanding of how tissue stress may lead to ectopic follicle formation. The finding of a correlation between chemokines and thyroid autoantibodies further suggests that intrathyroidal germinal centers play a significant role in the autoimmune response. Unexpectedly, the percentage of circulating CXCR4(+) T cells and CCR7(+) B and T cells (but not of CXCR5) was significantly reduced in PBMCs of patients with autoimmune thyroid disease when they were compared with their intrathyroidal lymphocytes. This systemic effect of active intrathyroidal lymphoid tissue emerges as a possible new marker of thyroid autoimmune disease activity. 相似文献