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On the chemiosmotic hypothesis and the nature of the mitochondrial protonmotive force 总被引:2,自引:0,他引:2
F H Malpress 《Journal of theoretical biology》1981,92(3):255-265
33.
We critically compared Marcusenius specimens from the mouth of the Cunene River on the Namibia/Angola border, a harsh desert environment on the Atlantic Ocean coast virtually devoid of aerial insects with aquatic larvae which are an important food item, with Marcusenius multisquamatus Kramer & Wink, 2013 from the escarpment region of that same river, in a relatively rich and productive subtropical savannah environment. River mouth specimens were differentiated in morphology and electric organ discharges, as determined by ANOVA/MANOVA comparisons, principal component and discriminant analyses on morphological and electrophysiological characters, and genetics, including sequences of the mitochondrial cytochrome b gene, indicating reproductive isolation. Specimens from the river mouth differed from M. multisquamatus, their closest relatives, by having a shorter snout, a smaller eye diameter, and smaller nares separation. River mouth specimens were also differentiated from other, increasingly less-close relatives, such as M. altisambesi Kramer et al., 2007 from the Okavango River, Botswana, and from M. krameri Maake et al., 2014 from the Limpopo System, South Africa. We therefore designate the new species Marcusenius desertus sp. nov. for the Cunene River mouth population. 相似文献
34.
F H Malpress 《Journal of theoretical biology》1984,109(4):523-532
Two hypotheses are compared each interpreting mitochondrial energy transduction in terms of a localized form of proton activity. Their differences are seen to be profound and far-reaching. It is concluded that the "chemiosmotic/local energized proton dialogue" as conducted hitherto has offered a very incomplete and restricted analysis of the problems of mitochondrial oxidative phosphorylation. 相似文献
35.
F H Malpress 《Journal of theoretical biology》1984,109(4):501-521
A coulombic hypothesis of mitochondrial oxidative phosphorylation is presented, founded upon the evidence for negative fixed charge formation during electron transport chain activity. The intermediary force is electrostatic (psi H) and not electrochemical (delta mu H). The electrochemical potential of the chemiosmotic hypothesis is identified as a "phantom" parameter which owes its delusive existence to the procedures by which it is measured. The connection between psi H and the conditional delta mu H values is examined; it entails the use of a variable conversion factor, f, where delta mu H (mV) = f psi H, and the concept of the "protonic status" of the diffuse double layer. A number of problems which beset the chemiosmotic view are reappraised in the light of the new interpretation, and find authentic solutions. 相似文献
36.
We report here the structural determination of N-linked oligosaccharides
found on extracellular hemoglobins of the hydrothermal vent tube worm
Riftia pachyptila. Structures were elucidated by a combination of
electrospray ionization tandem mass spectrometry, matrix- assisted laser
desorption/ionization mass spectrometry, normal-phase high performance
liquid chromatography, and exoglycosidase digestion. The sugar chains were
found to consist mainly of high-mannose-type glycans with some structures
partially capped by one or two terminal glucose residues. The present study
represents the first report of the occurrence of glucose capping of
N-linked carbohydrates in a secreted glycoprotein of a metazoan.
Previously, glucose capping has only been described for a membrane-bound
surface glycoprotein from the unicellular parasite Leishmania mexicana
amazonensis.
相似文献
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