Beta-adrenergic agonists and cyclic AMP decrease intracellular resting free-calcium concentration in ileum smooth muscle

Intracellular free-calcium levels were measured in strips of longitudinal smooth muscle from guinea-pig ileum; fura-2 was used as a calcium monitor. At rest the calcium concentration was about 180 nM, and this rose to 300-400 nM following electrical stimulation and during spontaneous calcium transients (all measurements at 23-25 degrees C). Isoprenaline suppressed the spontaneous calcium transients, and reduced the resting calcium level to about 130 nM. This fall in resting calcium concentration was seen even in muscle strips which did not have spontaneous activity. Elevation of intracellular cyclic AMP levels, produced by forskolin or dibutyryl cyclic AMP, mimicked the actions of isoprenaline. We conclude that the relaxant effects of beta-adrenergic agonists of visceral smooth muscle may be explained partly by a fall in intracellular resting free-calcium level, mediated via an increase in cyclic AMP.     

The effect of water-miscible solvents on the Δ1-dehydrogenase activity of free and PAAH-entrapped Arthrobacter simplex

Summary The effect of water-miscible cosolvents on biotransformations of poorly water-soluble substrates by immobilized cells was investigated, using ¹-dehydrogenation of hydrocortisone by Arthrobacter simplex as a model. Criteria for solvent selection on the basis of retention of enzymic activity were postulated and tested. Diols were considered to be the most suitable group of solvents. Substrate solubility increased tenfold in 30% (v/v) ethylene glycol, but reaction rates were significantly slower in such solutions. This was mainly caused by a decrease of oxygen solubility in the presence of the cosolvent and conformational changes imposed on the intracellular enzyme by cosolvent molecules penetrating the cell. The inhibition could be eliminated by the addition of an artificial electron acceptor, phenazine methosulphate (PMS). Reaction rates faster than those for substrate suspensions (no cosolvent added) could thus be achieved. Immobilization of Arthrobacter simplex in cross-linked polyacrylamide hydrazide gave high retentions of activity. PMS exhibited toxic effects on the entrapped cells, leading to reduced activity after extended use.     

Cation amelioration of aluminum toxicity in wheat

Aluminum is a major constituent of most soils and limits crop productivity in many regions. Amelioration is of theoretical as well as practical interest because understanding amelioration may contribute to an understanding of the mechanisms of toxicity. In the experiments reported here 2-day-old wheat (Triticum aestivum L. cv Tyler) seedlings with 15-millimeter roots were transferred to solutions containing 0.4 millimolar CaCl₂ at pH 4.3 variously supplemented with AlCl₃ and additional amounts of a chloride salt. Root lengths, measured after 2 days in the test solutions, were a function of both Al activity and the cation activity of the added salt. Percent inhibition = 100 {Al³⁺}/({Al³⁺} + K_m + α{C}^β) where {Al³⁺} is the activity of Al³⁺ expressed in micromolar, {C} is the activity of the added cation expressed in millimolar, and K_m (= 1.2 micromolar) is the {Al³⁺} required for 50% inhibition in the absence of added salt. For Ca²⁺, Mg²⁺, and Na⁺ the values of α were 2.4, 1.6, and 0.011, respectively, and the values for β were 1.5, 1.5, and 1.8, respectively. With regard to relative ameliorative effectiveness, Ca²⁺ > Mg²⁺ ≈ Sr²⁺ K⁺ ≈ Na⁺. Other cations were tested, but La³⁺, Sc³⁺, Li⁺, Rb⁺, and Cs⁺ were toxic at potentially ameliorative levels. The salt amelioration is not solely attributable to reductions in {Al³⁺} caused by increases in ionic strength. Competition between the cation and Al for external binding sites may account for most of the amelioration.     

Serial reconstruction and behaviour during cytokinesis of the chondriome ofProrocentrum minimum (Pavillard) Schiller (Pyrrhophyta)

Summary Serial reconstruction of the chondriome of vegetative cells ofProrocentrum minimum (Pavillard) Schiller has revealed a major reticulated mitochondrion and several small satellite mitochondria. During cytokinesis the major portion of the chondriome splits passively with the cell. The significance of this structure and division mechanism is discussed.