Post-nesting migrations patterns of Green Turtles (Chelonia mydas) from the Egyptian Red Sea

In order to identify the migratory pathways and foraging grounds of post-nesting Green Turtles (Chelonia mydas) in the Red Sea, we attached satellite transmitters to four females immediately after egg deposition and tracked them between 207 and 647 days. We identified four geographically distinct post-nesting habitat areas and migration paths from Zabargad Island, Egypt which was remarkable given our small sample size. Our shortest migration was 140?km and the longest 940?km, with the migrations and post-nesting habitat encompassing the boundaries of four of the seven Red Sea countries (Egypt, Sudan, Eritrea, and Saudi Arabia). The post-nesting habitats were located in shallow coastal habitat and three consisted of near-shore archipelagos. Two turtles moved past areas of suitable post-nesting habitat that was occupied by other turtles, which suggests that these turtles may be exhibiting fidelity to certain feeding and nesting sites. Our results suggest that regional and multi-national cooperation will be needed to protect sea turtles that nest on Zabargad Island, a nesting site that is important for Egypt and other Red Sea nations.     

Environmental impact and cost assessment of incineration and ethanol production as municipal solid waste management strategies

Purpose

Municipal solid waste (MSW) can be handled with several traditional management strategies, including landfilling, incineration, and recycling. Ethanol production from MSW is a novel strategy that has been proposed and researched for practical use; however, MSW ethanol plants are not widely applied in practice. Thus, this study has been conducted to analyze and compare the environmental and economic performance of incineration and ethanol production as alternatives to landfilling MSW.

Methods

The ISO 14040 life cycle assessment framework is employed to conduct the environmental impact assessment of three different scenarios for the two MSW management strategies based on processing 1 ton of MSW as the functional unit. The first scenario models the process of incinerating MSW and recovering energy in the form of process heat; the second scenario also includes the process of incinerating MSW but yields in the recovery of energy in the form of electricity; and the third scenario models the process of converting MSW into ethanol. The economic impacts of each scenario are then assessed by performing benefit-to-cost ratio (BCR) and net present value (NPV) analyses.

Results and discussion

The results from the environmental impact assessment of each scenario reveal that scenario 2 has the highest benefits for resource availability while scenario 3 is shown to be the best alternative to avoid human health and ecosystems diversity impacts. Scenario 1 has the worst environmental performance with respect to each of these environmental endpoint indicators and has net environmental impacts. The results of the economic analysis indicate that the third scenario is the best option with respect to BCR and NPV, followed by scenarios 2 and 1, respectively. Furthermore, environmental and economic analysis results are shown to be sensitive to MSW composition.

Conclusions

It appears municipalities should prefer MSW incineration with electricity generation or MSW-to-ethanol conversion over MSW incineration with heat recovery as an alternative to landfilling. The contradiction between the environmental impact assessment results and economic analysis results demonstrates that the decision-making process is sensitive to a broad set of variables. Decisions for a specific MSW management system are subject to facility location and size, MSW composition, energy prices, and governmental policies.     

Suppression of Powdery Mildew on Flax by Foliar Application of Essential Oils

Six essential oils were evaluated as to their efficiency in controlling powdery mildew (PM) of flax when they were applied as foliar sprays in an outdoor pot experiment. Onion, flax and fenugreek oils did not affect PM severity ratings – that is, they were ineffective in controlling the disease. On the other hand, black cumin, jojoba and coriander oil showed variable levels of efficiency in controlling the disease. Black cumin was moderately effective in controlling the disease because it reduced disease severity by 32.87%, while jojoba and coriander oils were highly effective as they reduced disease severity by 66.24 and 68.64%, respectively. Essential oils did not affect seed weight; however, coriander oil was a notable exception as it reduced seed weight by 55%. Straw weight was not affected by any oil. Foliar application of essential oils resulted in significant changes in the levels of protein, phenols, ascorbic acid and malondialdehyde and in activities of peroxidase and polyphenol oxidase. The lack of significant correlation between levels and activities of these biochemical components and PM severity demonstrate that these components are not involved in the suppression of PM by essential oils. Therefore, direct toxicity of essential oils to the causal pathogen Oidium lini is the most likely explanation for the disease suppression.     

The involvement of an endogenous circadian rhythm in photoperiodic timing in Acrochaetium asparagopsis (Rhodophyta,Acrochaetiales)

In Acrochaetium asparagopsis (Chemin) Papenfuss, tetrasporocyst formation is a genuine short-day photoperiodic response. Experiments in bi-diurnal and tri-diurnal cycles have shown that maximum inhibition of the response occurred with night-breaks given at approximately 24 h intervals and coincided with the postulated skotophile phase, while maximum stimulation was due to night-breaks in the postulated photophile phase. In cycle length experiments the fertility was stimulated in cycle lengths of 24 and 44 h and it was inhibited in cycle lengths of 36–40 and 52 h (or more). In addition the response was inhibited by light periods longer than 14 h, however long the associated dark period. Dark periods of various durations were completely ineffective in initiating tetrasporocysts and an induction phase of 5–7 short-days (8:) was necessary for the fertility. The lighting treatment following the induction phase was found to have no significant effect on the fertility and tetrasporocysts were obtained in continuous light or darkness. In resetting experiments, three more reversed short-day cycles (11 cycles, :8, photoperiod started at 24.00) were required to obtain the same maximum fertility (100%) observed under normal short-day cycles (9 cycles, 8:16, photoperiod started at 08.00). The induction, however, was completely lost with 96 h of continuous light (20 µE m^-2s^-1) and even 14 reversed short-day cycles were ineffective in initiating a new rhythmic mechanism.     

Metabolism of the phytoalexins camalexins,their bioisosteres and analogues in the plant pathogenic fungus Alternaria brassicicola

The metabolism of the phytoalexins camalexin (1), 1-methylcamalexin (10) and 6-methoxycamalexin (11) by Alternaria brassicicola and their antifungal activity is reported. This work establishes that camalexins are slowly biotransformed (ca. six days) to the corresponding indole-3-thiocarboxamides, which are further transformed to the indole-3-carboxylic acids. These metabolites are substantially less inhibitory to A. brassicicola than the parent camalexins, indicating that these enzyme-mediated transformations are detoxifications. In addition, analyses of the metabolism of synthetic isomers and bioisosteres of camalexin (1) indicate that isomers of camalexin in the thiazole ring are not metabolized. Based on these results, the potential intermediates that lead to formation of indole-3-thiocarboxamides are proposed.     

Arabidopsis PEX19 is a dimeric protein that binds the peroxin PEX10

Peroxisomes are organelles found in all eukaryotic cells. Peroxisomes import integral membrane proteins post-translationally, and PEX19 is a predominantly cytosolic, farnesylated protein of mammalian and yeast cells that binds multiple peroxisome membrane proteins and is required for their correct targeting/insertion to the peroxisome membrane. We report the characterisation of the Arabidopsis thaliana homologue of PEX19 which is a predominantly cytosolic protein. AtPEX19 is encoded by two genes (designated AtPEX19-1 and AtPEX19-2) that are expressed in all tissues and at all developmental stages of the plant. Quantitative real time PCR shows that AtPEX19-1 and AtPEX19-2 have distinct expression profiles. Using in vitro translation and co-immunoprecipitation AtPEX19-1 was shown to bind to the Arabidopsis peroxisomal membrane protein PEX10. Additionally, bacterially expressed recombinant AtPEX19-1 was able to bind a fusion protein consisting of the C-terminus of PEX10 and glutathione S-transferase in pull-down assays, thereby demonstrating that non-farnesylated AtPEX19 can interact with the C-terminus of AtPEX10. Purified recombinant AtPEX19-1 was analysed by gel filtration chromatography and was found to have a molecular weight consistent with it forming a dimer and a dimer was detected in Arabidopsis cell extracts that was slightly destabilised in the presence of DTT. Moreover, cross-linking studies of native AtPEX19 suggest that in vivo it is the dimeric species of the protein that preferentially forms complexes with other proteins.     

Deoxyribonucleic acid repair gene X-ray repair cross-complementing group 1 polymorphisms and non-carcinogenic disease risk in different populations: A meta-analysis

PURPOSE:

This study aims to assess a meta-analysis of the association of X-ray repair cross-complementing group 1 (XRCC1) polymorphisms with the risk of various non-carcinogenic diseases in different population.

MATERIALS AND METHODS:

This meta-analysis was performed by critically reviewing reveals 38 studies involving 10043 cases and 11037 controls. Among all the eligible studies, 14 focused on Arg194Trp polymorphism, 33 described the Arg399Gln and three articles investigated on Arg280His. Populations were divided into three different ethnic subgroups include Caucasians, Asians and other (Turkish and Iranian).

RESULTS:

Pooled results showed no correlation between Arg194Trp and non-carcinogenic disease. There was only weak relation in the recessive (odds ratio [OR] =1.11, 95% confidence interval [CI]: 0.86-1.44) model in Asian population and dominant (OR = 1.04, 95% CI: 0.66-1.63) model of other populations. In Arg399Gln polymorphism, there was no relation with diseases of interest generally. In the pooled analysis, there were weak relation in the dominant (OR = 1.08, 95% CI: 0.86-1.35) model of Asian population and quite well-correlation with recessive (OR = 1.49, 95% CI: 1.19-1.88), dominant (OR = 1.23, 95% CI: 0.94-1.62), and additive (OR = 1.23, 95% CI: 0.94-1.62) models of other subgroup. For Arg280His, there was a weak relation only in the dominant model (OR = 1.06, 95% CI: 0.74-1.51).

CONCLUSION:

The present meta-analysis correspondingly shows that Arg399Gln variant to be associated with increased non-carcinogenic diseases risk through dominant and recessive modes among Iranian and Turkish population. It also suggests a trend of dominant and recessive effect of Arg280His variant in all population and its possible protective effect on non-carcinogenic diseases.     

Comparative physiological and biochemical evaluation of salt and nickel tolerance mechanisms in two contrasting tomato genotypes

Plant tolerance against a combination of abiotic stresses is a complex phenomenon, which involves various mechanisms. Physiological and biochemical analyses of salinity (NaCl) and nickel (Ni) tolerance in two contrasting tomato genotypes were performed in a hydroponics experiment. The tomato genotypes selected were proved to be tolerant (Naqeeb) and sensitive (Nadir) to both salinity and Ni stress in our previous experiment. The tomato genotypes were exposed to combinations of NaCl (0, 75 and 150 mM) and Ni (0, 15, and 20 mg l⁻¹) for 28 days. The results revealed that the tolerant and sensitive tomato genotypes showed similar response to NaCl and Ni stress; however, the level of response was significantly different in both genotypes. The tolerant tomato genotype showed less reduction in growth than the sensitive genotype against both NaCl and Ni stress. Root and shoot ionic analysis showed a decrease in Na and increase in K concentration by increasing Ni levels in the growth medium. Moreover, accumulation of Na and Ni in tissues showed a decrease in membrane stability index and an increase in malondialdehyde contents. The activity of superoxide dismutase, catalase, peroxidase and glutathione reductase under NaCl and Ni stress was significantly higher in the tolerant compared to the sensitive genotype. Enhanced activity of many antioxidant enzymes in Naqeeb under stress conditions is among the other mechanisms that enabled the genotype to better detoxify reactive oxygen species and therefore Naqeeb tolerated the stresses better than Nadir.     

Multi-strain Inoculation with PGPR Producing ACC Deaminase is More Effective Than Single-strain Inoculation to Improve Wheat (<i>Triticum aestivum</i>) Growth and Yield

Rhizosphere bacteria that colonize plant roots and confer beneficial effects are referred as plant growth promoting rhizobacteria (PGPR). Among all PGPR, some rhizobacteria have an ability to produce ACC deaminase enzyme. This enzyme catalyzes stress ACC into a-ketobutyrate and ammonia instead of letting it to be converted to ethylene. Ethylene level rises in plants under stress conditions i.e., drought, salinity, poor soil fertility etc. As poor soil fertility is a big hurdle to achieve the optimum yield of crops, inoculation of ACC deaminase PGPR can overcome this problem to some extent. The aim of the current study was to examine the influence of multi-strain and single-strain inoculation of different ACC deaminase producing PGPR on wheat growth and yield. There were three PGPR strains, Enterobacter cloacae, Serratia ficaria and Burkholderia phytofirmans which were used as consortia and single-strain inoculations. The results showed that inoculation of E. cloacae + S. ficaria + B. phytofirmans significantly increased plant height (63%), spike length (61%), number of spikelets spike^-1 (61%), number of grains spike-1 (131%), 1000 grains weight (33%), grains yield (71%), straw yield (71%) and biological yield (68%) of wheat as compared to control. A significant improvement in N (37 and 200%), P (46 and 166%) and K (39 and 61%) of seeds and shoot respectively, validated the efficacious and more effective role of multi-strain (E. cloacae + S. ficaria + B. phytofirmans) inoculation over control. It is obviously concluded that multi-strain ACC deaminase producing PGPR inoculation is a better approach as compared to singlestrain inoculation for the improvement in growth and yield of wheat.