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71.
72.
The literature data on the activity of histidine-15 modified hen egg white lysozyme are conflicting: the modified enzyme is
reported to have more activity, similar activity or less activity by different authors. Amino acid analysis had shown modification
of the single His-15. Detailed activity studies on His-15-modified (by iodoacetic acid or diethyl pyrocarbonate) lysozyme
have shown that the contradicting reports are due to the specific choices of ionic strengths and cell wall substrate concentrations
and can be attributed to the substrate being negatively charged. Our analysis suggests that even though histidine-15 is far
removed from the active site of lysozyme, its chemical modification or binding of the negatively-charged substrate near it,
changes the conformation around the active site. However, the change in the optimum activity on chemically modifying His-15
is small. 相似文献
73.
M. R. Chaurasia 《Bioscience, biotechnology, and biochemistry》2013,77(6):711-714
The syntheses and the properties are reported for a series of 6-iodo-2-alkylthio-3-aryl (or alkyl)-4-(3H) quinazolones of interest as potential ataractic agents and antimalarials. 相似文献
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77.
Yang W Bhandaru M Pasham V Bobbala D Zelenak C Jilani K Rotte A Lang F 《Cellular physiology and biochemistry》2012,29(1-2):21-30
The anti-inflammatory Nigella sativa component thymoquinone compromises the function of dendritic cells (DCs), key players in the regulation of innate and adaptive immunity. DC function is regulated by the Na(+)/H(+) exchanger (NHE), which is stimulated by lipopolysaccharides (LPS) and required for LPS-induced cell swelling, reactive oxygen species (ROS) production, TNF-α release and migration. Here we explored, whether thymoquinone influences NHE activity in DCs. To this end, bone marrow derived mouse DCs were treated with LPS in the absence and presence of thymoquinone (10 μM). Cytosolic pH (pH(i)) was determined from 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) fluorescence, NHE activity from the Na(+)-dependent realkalinization following an ammonium pulse, cell volume from forward scatter in FACS analysis, ROS production from 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) fluorescence, TNF-α production utilizing ELISA and DC migration with transwell migration assays. As a result, exposure of DCs to LPS (1 μg/ml) led within 4 hours to transient increase of NHE activity. Thymoquinone did not significantly modify cytosolic pH or cellular NHE activity in the absence of LPS, but abrogated the effect of LPS on NHE activity. Accordingly, in the presence of thymoquinone LPS-treatment resulted in cytosolic acidification. LPS further increased forward scatter and ROS formation, effects similarly abrogated by thymoquinone. Again, in the absence of LPS, thymoquinone did not significantly modify ROS formation and cell volume. LPS further triggered TNF-α release and migration, effects again blunted in the presence of thymoquinone. NHE1 inhibitor cariporide (10 μM) blunted LPS induced TNF-α release and migration. The effects of thymoquinone on NHE activity and migration were reversed upon treatment of the cells with t-butyl hydroperoxide (TBOOH, 5 μM). In conclusion, thymoquinone blunts LPS induced NHE activity, cell swelling, oxidative burst, cytokine release and migration of bone marrow derived murine dendritic cells. NHE inhibition may thus contribute to the antiinflammatory action of thymoquinone. 相似文献
78.
Biju Bhargavan Abnish Kumar Gautam Divya Singh Amit Kumar Sumit Chaurasia Abdul Malik Tyagi Dinesh Kumar Yadav Jay Sharan Mishra Amar Bahadur Singh Sabyasachi Sanyal Atul Goel Rakesh Maurya Naibedya Chattopadhyay 《Journal of cellular biochemistry》2009,108(2):388-399
Following a lead obtained from stem‐bark extract of Butea monosperma, two structurally related methoxyisoflavones; cajanin and isoformononetin were studied for their effects in osteoblasts. Cajanin had strong mitogenic as well as differentiation‐promoting effects on osteoblasts that involved subsequent activation of MEK‐Erk and Akt pathways. On the other hand, isoformononetin exhibited potent anti‐apoptotic effect in addition to promoting osteoblast differentiation that involved parallel activation of MEK‐Erk and Akt pathways. Unlike genistein or daidzein, none of these two compounds appear to act via estrogen receptors in osteoblast. Once daily oral (by gavage) treatment for 30 consecutive days was given to recently weaned female Sprague–Dawley rats with each of these compounds at 10.0 mg kg?1 day?1 dose. Cajanin increased bone mineral density (BMD) at all skeletal sites studied, bone biomechanical strength, mineral apposition rate (MAR) and bone formation rate (BFR), compared with control. BMD levels at various anatomic positions were also increased with isoformononetin compared with control however, its effect was less potent than cajanin. Isoformononetin had no effect on the parameters of bone biomechanical strength although it enhanced MAR and BFR compared with control. Isoformononetin had very mild uterotrophic effect, whereas cajanin was devoid of any such effect. Our data suggest that cajanin is more potent than isoformononetin in accelerating peak bone mass achievement. To the best of our knowledge, this work represents the first attempt to elucidate structure‐activity relationship between the two methoxylated isoflavones regarding their effects in osteoblasts and bone formation. J. Cell. Biochem. 108: 388–399, 2009. © 2009 Wiley‐Liss, Inc. 相似文献
79.
Akhilesh Kumar Chaurasia Shree Kumar Apte 《Applied and environmental microbiology》2009,75(18):6008-6012
The bicistronic groESL operon, encoding the Hsp60 and Hsp10 chaperonins, was cloned into an integrative expression vector, pFPN, and incorporated at an innocuous site in the Anabaena sp. strain PCC7120 genome. In the recombinant Anabaena strain, the additional groESL operon was expressed from a strong cyanobacterial PpsbA1 promoter without hampering the stress-responsive expression of the native groESL operon. The net expression of the two groESL operons promoted better growth, supported the vital activities of nitrogen fixation and photosynthesis at ambient conditions, and enhanced the tolerance of the recombinant Anabaena strain to heat and salinity stresses.Nitrogen-fixing cyanobacteria, especially strains of Nostoc and Anabaena, are native to tropical agroclimatic conditions, such as those of Indian paddy fields, and contribute to the carbon (C) and nitrogen (N) economy of these soils (22, 30). However, their biofertilizer potential decreases during exposure to high temperature, salinity, and other such stressful environments (1). A common target for these stresses is cellular proteins, which are denatured and inactivated during stress, resulting in metabolic arrest, cessation of growth, and eventually loss of viability. Molecular chaperones play a major role in the conformational homeostasis of cellular proteins (13, 16, 24, 26) by (i) proper folding of nascent polypeptide chains; (ii) facilitating protein translocation and maturation to functional conformation, including multiprotein complex assembly; (iii) refolding of misfolded proteins; (iv) sequestering damaged proteins to aggregates; and (v) solubilizing protein aggregates for refolding or degradation. Present at basal levels under optimum growth conditions in bacteria, the expression of chaperonins is significantly enhanced during heat shock and other stresses (2, 25, 32).The most common and abundant cyanobacterial chaperones are Hsp60 proteins, and nitrogen-fixing cyanobacteria possess two or more copies of the hsp60 or groEL gene (http://genome.kazusa.or.jp/cyanobase). One occurs as a solitary gene, cpn60 (17, 21), while the other is juxtaposed to its cochaperonin encoding genes groES and constitutes a bicistronic operon groESL (7, 19, 31). The two hsp60 genes encode a 59-kDa GroEL and a 61-kDa Cpn60 protein in Anabaena (2, 20). Both the Hsp60 chaperonins are strongly expressed during heat stress, resulting in the superior thermotolerance of Anabaena, compared to the transient expression of the Hsp60 chaperonins in Escherichia coli (20). GroEL and Cpn60 stably associate with thylakoid membranes in Anabaena strain PCC7120 (14) and in Synechocystis sp. strain PCC6803 (15). In Synechocystis sp. strain PCC6803, photosynthetic inhibitors downregulate, while light and redox perturbation induce cpn60 expression (10, 25, 31), and a cpn60 mutant exhibits a light-sensitive phenotype (http://genome.kazusa.or.jp/cyanobase), indicating a possible role for Cpn60 in photosynthesis. GroEL, a lipochaperonin (12, 28), requires a cochaperonin, GroES, for its folding activity and has wider substrate selectivity. In heterotrophic nitrogen-fixing bacteria, such as Klebsiella pneumoniae and Bradyrhizobium japonicum, the GroEL protein has been implicated in nif gene expression and the assembly, stability, and activity of the nitrogenase proteins (8, 9, 11).Earlier work from our laboratory demonstrated that the Hsp60 family chaperonins are commonly induced general-stress proteins in response to heat, salinity, and osmotic stresses in Anabaena strains (2, 4). Our recent work elucidated a major role of the cpn60 gene in the protection from photosynthesis and the nitrate reductase activity of N-supplemented Anabaena cultures (21). In this study, we integrated and constitutively overexpressed an extra copy of the groESL operon in Anabaena to evaluate the importance and contribution of GroEL chaperonin to the physiology of Anabaena during optimal and stressful conditions.Anabaena sp. strain PCC7120 was photoautotrophically grown in combined nitrogen-free (BG11−) or 17 mM NaNO3-supplemented (BG11+) BG11 medium (5) at pH 7.2 under continuous illumination (30 μE m−2 s−1) and aeration (2 liters min−1) at 25°C ± 2°C. Escherichia coli DH5α cultures were grown in Luria-Bertani medium at 37°C at 150 rpm. For E. coli DH5α, kanamycin and carbenicillin were used at final concentrations of 50 μg ml−1 and 100 μg ml−1, respectively. Recombinant Anabaena clones were selected on BG11+ agar plates supplemented with 25 μg ml−1 neomycin or in BG11− liquid medium containing 12.5 μg ml−1 neomycin. The growth of cyanobacterial cultures was estimated either by measuring the chlorophyll a content as described previously (18) or the turbidity (optical density at 750 nm). Photosynthesis was measured as light-dependent oxygen evolution at 25 ± 2°C by a Clark electrode (Oxy-lab 2/2; Hansatech Instruments, England) as described previously (21). Nitrogenase activity was estimated by acetylene reduction assays, as described previously (3). Protein denaturation and aggregation were measured in clarified cell extracts containing ∼500 μg cytosolic proteins treated with 100 μM 8-anilino-1-naphthalene sulfonate (ANS). The pellet (protein aggregate) was solubilized in 20 mM Tris-6 M urea-2% sodium dodecyl sulfate (SDS)-40 mM dithiothreitol for 10 min at 50°C. The noncovalently trapped ANS was estimated using a fluorescence spectrometer (model FP-6500; Jasco, Japan) at a λexcitation of 380 nm and a λemission of 485 nm, as described previously (29).The complete bicistronic groESL operon (2.040 kb) (GenBank accession no. ) was PCR amplified from PCC7120 genomic DNA using specific primers (Table FJ608815(Table1)1) and the amplicon cloned into the NdeI-BamHI restriction sites of plasmid vector pFPN, which allows integration at a defined innocuous site in the PCC7120 genome and expression from a strong cyanobacterial PpsbA1 promoter (6). The resulting construct, designated pFPNgro (Table (Table1),1), was electroporated into PCC7120 using an exponential-decay wave form electroporator (200 J capacitive energy at a full charging voltage of 2 kV; Pune Polytronics, Pune, India), as described previously (6). The electroporation was carried out at 6 kV cm−1 for 5 ms, employing an external autoclavable electrode with a 2-mm gap. The electroporation buffer contained high concentrations of salt (10 mM HEPES, 100 mM LiCl, 50 mM CaCl2), as have been recommended for plant cells (23) and other cell types (27). The electrotransformants, selected on BG11+ agar plates supplemented with 25 μg ml−1 neomycin by repeated subculturing for at least 25 weeks to achieve complete segregation, were designated AnFPNgro.
Open in a separate windowaThe underlined nucleotides in the primer sequences represent the incorporated restriction endonuclease sites.The transfer of pFPNgro to PCC7120 resulted in the integration of an extra copy of groESL (PpsbA1-groESL) into the PCC7120 genome. PCR amplification (Fig. (Fig.1I)1I) with the PpsbA1 forward and groESL reverse primer pairs showed the additional copy of groEL juxtaposed downstream to the PpsbA1 promoter (lane 6) in the recombinant Anabaena strain, while the native groESL operon found in the wild-type strain (lane 3) remained intact in the AnFPNgro strain (lane 5).Open in a separate windowFIG. 1.Integration and constitutive expression of an additional groESL operon in Anabaena strain PCC7120. (I) Integration of an additional groESL operon in the PCC7120 genome. The electrophoretogram shows the transfer and integration of PpsbA1-groESL in strain AnFPNgro. Lane 1, 1-kb DNA marker; lane 2, PCR control template without primer; lane 3, PCR product from wild-type Anabaena using the groESLfwd and groESLrev primers; lane 4, PCR product from PCC7120 using the PpsbA1fwd and groESLrev primers; lane 5, PCR product from AnFPNgro using the groESLfwd and groESLrev primers; lane 6, PCR product from AnFPNgro using the PpsbA1fwd and groESLrev primers. (II) Expression of the groESL operon in the wild-type and recombinant Anabaena strains during stress. PCC7120 (An7120) and AnFPNgro were grown for 3 days and then subjected to either heat stress (42°C) for 4 h (A and A′) or salinity stress (150 mM NaCl) for 3 days (B and B′). GroEL levels were estimated by Western blotting of 10% SDS-polyacrylamide gel electrophoresis-resolved whole-cell proteins, followed by immunodetection using anti-AnGroEL antiserum and densitometry (A and B). Panels A′ and B′ depict SDS-polyacrylamide gel electrophoresis-resolved and Coomassie blue-stained proteins to show equal sample loading. Various lanes contained protein samples under unstressed-control (U), heat (H), or salt (S) stress conditions. Numbers below panels A and B show GroEL quantitation by densitometry.Under normal growth conditions, the recombinant AnFPNgro cells expressed about 8.7- to 9.9-fold higher levels of GroEL protein than that detected in the PCC7120 cells (Fig. 1II), indicating a strong constitutive expression of the GroEL protein from the PpsbA1 promoter. In PCC7120, the wild-type copy of the GroEL protein was induced by both heat shock (Fig. 1IIA, lane 2) and salt stress (Fig. 1IIB, lane 2). GroEL levels in the recombinant strain were found to be about 2.5-fold higher under heat stress (Fig. 1IIA, lane 4) and approximately 1.7-fold higher under salinity stress (Fig. 1IIB, lane 4) than that expressed by PCC7120 under these stresses (Fig. 1IIA and IIB, lanes 2). The exposure of AnFPNgro cells to heat stress resulted in a further increase of approximately sixfold in GroEL levels (Fig. 1IIA, lane 4), while salt stress enhanced GroEL levels by approximately threefold (Fig. 1IIB, lane 4), compared to the constitutively expressed GroEL level in this strain (Fig. 1IIA and IIB, lanes 3). The constitutive expression of GroEL protein in AnFPNgro under ambient conditions (Fig. 1IIA and IIB, lanes 3) was from the PpsbA1 promoter (Fig. (Fig.1I,1I, lane 6). We assume that the additional increase in GroEL levels observed under heat and salt stress (Fig. 1IIA and IIB, lanes 4) was due to the native stress-induced groESL operon, functional from its own promoter.The diazotrophically grown PCC7120 did not grow during prolonged exposure to heat stress (42°C) (Fig. (Fig.2A)2A) and showed poor growth during salinity stress (150 mM) (Fig. (Fig.2B).2B). Salinity stress was particularly severe for photosynthetic pigments in PCC7120 and bleached the cells (data not shown). In contrast, the recombinant strain AnFPNgro showed a higher content of major photosynthetic pigments (Fig. (Fig.2C)2C) and presented a healthier blue-green phenotype (data not included). Strain AnFPNgro also showed better growth than wild-type PCC7120, both under unstressed and stressed conditions (Fig. 2A and B).Open in a separate windowFIG. 2.Effect of groESL overexpression on thermotolerance and salinity tolerance of diazotrophically grown Anabaena strains. (A) Growth (measured as chlorophyll a content) of strains during prolonged exposure to 42°C. (B) Growth (turbidity measured at an optical density at 750 nm) during prolonged exposure to 150 mM NaCl. (C) Absorption spectra of a dilute suspension of whole filaments after 7 days of exposure to various NaCl concentrations.The photosynthetic activity decreased with time during heat stress in PCC7120 but was maintained at comparatively higher levels in AnFPNgro cells (Fig. (Fig.3A)3A) than in PCC7120. The dinitrogenase activity in PCC7120 was severely inhibited after 4 h of heat stress (Fig. (Fig.3B).3B). In contrast, the dinitrogenase activity of the recombinant strain (AnFPNgro) was about 1.5-fold higher than PCC7120 under ambient conditions (25°C ± 2°C, no NaCl) and more than 3-fold higher than that of PCC7120 after 4 h of heat stress (Fig. (Fig.3B).3B). Prolonged exposure to salinity stress inhibited photosynthesis and nitrogen fixation in PCC7120 (Fig. 3C and D). However, strain AnFPNgro displayed significant protection of these activities, possibly due to overexpressed GroES/GroEL proteins. The recombinant strain (AnFPNgro) exhibited much-reduced protein aggregation after 4 h of heat stress or after prolonged exposure (10 days) to salinity stress than PCC7120 (Fig. (Fig.44).Open in a separate windowFIG. 3.Effect of groESL overexpression on photosynthesis and nitrogen fixation in Anabaena. Photosynthesis (A and C) and nitrogenase activity (B and D) in wild-type Anabaena strain PCC7120 (An7120) and recombinant AnFPNgro strains exposed to heat stress for 10 days (A) or 4 h (B) or to salinity stress (150 mM) for 10 days (C and D). Letters U, H, and S denote unstressed-control, heat stress, and salt stress conditions, respectively.Open in a separate windowFIG. 4.Protein aggregation in Anabaena strains during exposure to heat and salinity stress. The protein aggregation was monitored by ANS fluorescence after 4 h of exposure to 42°C (H) or 10 days of exposure to 150 mM NaCl (S) and compared with the unstressed controls (U) of recombinant strain AnFPNgro and the wild-type Anabaena strain PCC7120 (An7120). The fluorescence intensity output from the spectrofluorimeter is expressed as arbitrary units (a.u.).This study evaluated the possible benefits of groESL overexpression for the general stress tolerance of PCC7120. The recombinant AnFPNgro strain harbored two groESL operons, one native stress-inducible groESL and a second groESL operon integrated at a defined innocuous site and placed downstream of a constitutive PpsbA1 promoter (Fig. (Fig.1).1). The recombinant AnFPNgro strain showed an 8- to 10-fold higher constitutive expression of GroEL under ambient conditions than PCC7120, while its inherent stress-induced GroEL expression was not impaired and resulted in 30- and 48-fold more GroEL under salt and heat stress, respectively (Fig. (Fig.11).The AnFPNgro cells exhibited better growth (Fig. (Fig.2),2), photosynthesis, and nitrogen fixation (Fig. (Fig.3)3) than PCC7120, suggesting a possible limitation on the availability of GroEL under ambient conditions. The protection of photosynthetic pigments and oxygen photoevolution during salinity stress were particularly impressive. Nearly 2- to 2.5-fold higher GroEL levels in AnFPNgro under heat or salt stress, compared to those of PCC7120 (Fig. (Fig.1),1), lowered the stress-triggered protein aggregation (Fig. (Fig.4)4) and had beneficial consequences for photosynthesis and nitrogen fixation in the recombinant strain (Fig. (Fig.3).3). An overall improvement in the aforesaid vital metabolic activities eventually resulted in the superior tolerance of recombinant AnFPNgro to heat and salt stresses. 相似文献
TABLE 1.
Plasmids, strains, and primers used in this studyPlasmid, strain, or primer | Feature or sequencea | Source or reference |
---|---|---|
Plasmids | ||
pFPN | Integrative expression vector | 6 |
pFPNgro | pFPN with groESL operon | This study |
Strains | ||
An7120 | Wild-type Anabaena sp. strain PCC7120 | R. Haselkorn |
AnFPNgro | GroESL-overexpressing Anabaena | This study |
Primers | ||
groESLfwd | 5′-GGA ATT CCA TAT GGC AGC AGT ATC TCT AAG-3′ | This study |
groESLrev | 5′-CGC GGA TCC TTA GTA ATC GAA GTC ACC GCC-3′ | This study |
PpsbA1fwd | 5′-GAG CTG CAG GGA TTC CCA AAG ATA GGG-3′ | 6 |
PpsbA1rev | 5′-CTC GGA TCC CCA TAT GTT TTT ATG ATT GCT TTG-3′ | 6 |
80.