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91.
92.
G. Lushai T.N. Sherratt O. David P.J. De Barro N. Maclean 《Entomologia Experimentalis et Applicata》1997,85(3):199-209
A total of 55 parthenogenetic lineages of the grain aphid, Sitobion avenae F. were established from single clones collected from winter wheat (agricultural host) and cocksfoot (natural host) at various locations in southern Britain. RAPD-PCR profiles indicated that these lineages consisted of at least 15 genetically distinct clones. Twenty winged individuals (alatae) of known weight were taken from each lineage and presented with a choice of wheat and cocksfoot hosts (with a total leaf area each of 2 cm2) held in petri dishes (laboratory experiments) and flowerpots (field experiments). In both experimental designs host preferences were determined by a ranking of a proportion of counts (limited to -1 +1) of the progeny produced on each host after 5 days. Overall, alatae tended to prefer the agricultural host. However, alatae from individual clones found exclusively on wheat generally had a higher preference for wheat than alatae from individual clones found exclusively on cocksfoot. Wheat-derived lineages (aphid genotypes that were collected on wheat but also present on cocksfoot) showed a significantly greater preference for the agricultural host than the cocksfoot-derived lineages. Individuals from a wheat-derived lineage had significantly higher observed and potential progeny production on wheat than they did on cocksfoot, while individuals from a cocksfoot-derived lineage had significantly higher potential progeny but lower mean progeny weights on wheat. In a second stage, reciprocal host transfer experiments were carried out in the laboratory, i.e. lineages collected from the agricultural host were reared for several generations on the natural host and vice versa prior to being tested for host preference. The preference of the lineages for their host of origin significantly decreased in this second trial, reversing the overall preference trends, while there was little evidence for between-lineage variation in this change in preference. In summary these results indicate weak genotypic but strong environmental influences on alate host preference in S. avenae. This host plant conditioning effect may serve to promote host-based genetic structuring observed in southern British populations of S. avenae. 相似文献
93.
94.
Domenico Davolos Norman Maclean Biancamaria Pietrangeli 《Theoretical biology forum》2004,97(1):161-168
In this paper we performed a molecular phylogenetic study of Orchestia cavimana, the sole talitrid amphipod inhabiting beaches of European freshwater lakes and rivers. For that purpose, we have PCR amplified and sequenced regions of the mitochondrial cytochrome oxidase subunit I (COI) gene, basing our analysis on both nucleotide and amino acid sequences and considering also structural classes of the COI enzyme. Phylogenetic analyses were conducted by neighbour-joining (NJ) and maximum-parsimony (MP) methods comparing homologous sequences of talitrids and other Crustacea. In both NJ and MP trees, O. cavimana shows a basal placement with respect to other talitrid amphipods. 相似文献
95.
Astrocytes execute essential functions in the healthy CNS, whilst also being implicated as a limitation to functional regeneration and repair after injury. They respond to injury to minimize damage to healthy tissue whilst also attempting to seal the broken blood-brain-barrier, however, they impede recovery if they are persistent and form a permanent scar in the injured brain. As such, it is of great importance to understand the mechanism underlying the astrocytic response to injury, and this understanding is currently limited by the in vitro environments available to scientists. Biomaterials such as nanofibres and hydrogels offer great potential for the development of superior, 3D cell culture environments in which to study astrocyte behavior and phenotype. The implementation of such in vitro environments with a particularly interdisciplinary approach can improve the field’s understanding of astrocytes, their role in central nervous system inflammation, and elucidate potential strategies to achieve functional regeneration. 相似文献
96.
The haemoglobins and globins of the roach, Rutilus rutilus (L.) and the rudd, Scardinius erythrophthalmus (L.) have been studied by starch gel electrophoresis. At all stages of development studied, the roach and rudd haemoglobins give a similar electrophoretic pattern, the young fish of about 2 cm standard length possessing three separate haemoglobins, of which two bands only persist in the adult. The evolutionary significance of multiple haemoglobins in fishes is briefly discussed. 相似文献
97.
P. Agnellus Schneider Reinhard Hoppe G. Timmermann G. L. Maclean Hans Deetjen H. Deetjen F. Schmitz Detlef Robel Herbert Ringleben Vesta Stresemann Erwin R. Scherner Eckart Schwarze H. Kumerloeve Heinrich Dathe und Wolfgang Fischer 《Journal of Ornithology》1969,110(1):101-112
Zusammenfassung Die Schlußfolgerungen, dieStegmann (J. Orn. 109, 1968, p. 441–445) für die verwandtschaftliche Stellung der Flughühner auf Grund seiner eigenen Untersuchungen und an Hand anderer Quellen zieht, werden kommentiert. Es wird gezeigt, daß einige dieser Schlußfolgerungen nur auf morphologischen Merkmalen beruhen und mit den zu anderen Resultaten gelangten ethologischen und physiologischen Untersuchungsergebnissen nicht zu vereinbaren sind. Außerdem erscheint es unwahrscheinlich, daß die Flughühner, deren Ursprung von bodenlebenden Nestflüchtern unbestritten ist, auf dem Umweg über die baum- und felsbewohnenden nesthockenden Tauben erst sekundär wieder zu bodenbewohnenden Nestflüchtern geworden sind. Es gibt kein einziges Flughuhn-Merkmal, das diese Hypothese stützen könnte. 相似文献
98.
99.
Human alveolar macrophage fibronectin: synthesis, secretion, and ultrastructural localization during gelatin-coated latex particle binding 总被引:14,自引:4,他引:10 下载免费PDF全文
Human pulmonary alveolar macrophages synthesized and secreted several characteristic high molecular weight proteins for at least 7 d in vitro. Immunoprecipitates of medium and cell lysates from metabolically labeled cultures with specific anti-human plasma fibronectin IgG contained one major labeled polypeptide of molecular weight 440,000 (unreduced) or 220,000 (reduced). An identical polypeptide in conditioned medium from radiolabeled macrophages bound specifically to gelatin-Sepharose, demonstrating that alveolar macrophages synthesized and secreted a molecule immunologically and functionally similar to fibronectin. Fibronectin was the major newly synthesized and secreted polypeptide of freshly harvested alveolar macrophages. Pulse-chase experiments revealed that newly synthesized fibronectin was rapidly secreted into medium, approximately 50 percent appearing by 1 h and 80 percent by 8 h. Immunoperoxidase staining using antifibronectin F(ab’)(2)-peroxidase conjugates revealed the majority of immunoreactive fibronectin to be intracellular, localized to endoplasmic reticulum and Golgi apparatus. No extracellular matrix fibronectin was visualized, and cell surface staining was rarely seen, usually appearing only at sites where cells were closely apposed and not at sites of macrophage-substrate attachment. Similar immunostaining of fibroblast cultures revealed cell surface-associated fibrillar fibronectin. Ultrastructural localization of fibronectin during binding and phagocytosis of gelatin-coated and plain latex particles revealed fibronectin only on gelatin-latex beads and at their cell binding sites. Neigher plain latex beads nor their cell membrane binding sites stained for fibronectin. These results demonstrate that fibronectin is a major product of human alveolar macrophages, is rapidly secreted, and is localized at cell membrane binding sites for gelatin-coated particles. In view of the known binding properties of fibronectin, it may serve as an endogenous opsonic factor promoting the binding of staphylococcus, denatured collagen, fibrin, or other macromolecules to macrophages in the lower respiratory tract. 相似文献
100.
Shirley Maclean 《Cell and tissue research》1980,210(3):435-445
Summary The histological and ultrastructural organisation of the epidermal sensory organs in Amphibolurus barbatus has been described with respect to their position and possible functions. The sensory organs, located at the scale's edge, are most numerous in scales of the dorsal surface of the head. Most other scales of the body surface have two receptors located laterally to the spine or keel of the scale. In the imbricate scales of the ventral body region, the receptors lie just beneath the reinforced scale lip. Scanning electron microscopy has revealed the surface of the organ to be a crater lacking any surface projections. These sensory organs have a dermal papilla consisting of a nerve plexus and loose connective tissue. The nerve fibres arising from the plexus, pass to the epidermal columnar cells, where some form nerve terminals at the base of the cells, while others pass between them to form nerve terminals embedded in a superficial layer of cuboidal cells. The superficial terminals are held against the overlying keratin by masses of tonofilaments. The keratin is thickened to form a collar around the periphery of the organ but is only about 0.5 m thick immediately above it. Mechanical deformation of the scale's spine or reinforced scale lip may initiate stimulation of the nerve terminals described. 相似文献