Sulla validità del genere Torulaspora

Zusammenfassung An 20 Stämmen von Torulaspora Rosei und 1 Stamm von Zygosaccharomyces globiformis forma typica wurde insbesondere die Sporenbildung untersucht. Da diese bei jenen immer parthenogenetisch erfolgt, muß die Gattung Torulaspora aufrecht erhalten werden.     

Polychaete populations of the shallow soft bottoms off Terra Nova Bay (Ross Sea, Antarctica): distribution, diversity and biomass

 Diversity, distribution and biomass patterns of polychaetes were studied in the shallow soft bottoms off Terra Nova Bay (Ross Sea, Antarctica). Benthic samples, collected in the austral summer 1989–1990, were sampled using a Van Veen grab at 14 stations distributed between 23- and 194-m depth. A total of 5,768 individuals of polychaetes belonging to 77 species were found. The species richness recorded was high, despite the few biotopes investigated at Terra Nova Bay in this study, especially when compared to that recorded from previous investigations in the same area and from the whole Ross Sea (146 species). Despite the high number of species found, only a few species accounted for 76% of the total abundance: Tharyx cincinnatus (27%), Spiophanes tcherniai (23%), Leitoscoloplos mawsoni (18%) and Laonice weddellia (5%) were mainly distributed in the deeper stations (below 100-m depth), while Aglaophamus ornatus (3%) was the only species abundant in the shallowest stations (above 50-m depth), characterized by gravels and coarse sands. Species richness, diversity and evenness showed an increase with depth. Abundances ranged between 40 to over 12,000 ind/m². Biomass values ranged between 0.9 to 176 g/m² (wet weight). A trophic-guild classification revealed the occurrence of various feeding categories that were, however, numerically dominated by deposit feeders with both discretely motile epiand endobenthic forms, and sessile ones. Both the Bray-Curtis similarity and Correspondence (C.A.) analyses showed stations ordered according to depth and to the local dominance of some species. Both poorly and well-structured polychaete assemblages occurred and they seemed related to depth-related factors, such as degree of environmental disturbance (shallowest bottoms vs deep ones), heterogeneity of the substrate and habitat complexity (gravel and coarse sands vs mixed sediment. The richest and most diverse assemblages of polychaetes were those occurring below 140-m depth, where the substrate was dominated by medium sands, often mixed with a mosaic of rocks and boulders. Received: 30 October 1995/Accepted: 1 May 1996     

(Na+-K+)-ATPase interaction with L-ascorbic acid. Effect on p-nitrohenylphosphatase partial reaction

L-ascorbic acid preincubation with rabbit kidney (Na+-K+)-ATPase inhibits the activity of p-nitrophenylphosphatase partial reaction with a pseudo-first order decay. The presence of the pseudosubstrate, p-nitrophenylphosphate, counteracts the inhibiting effect. During the reaction course, the kinetic rate is enhanced at ascorbic acid concentrations below 0.7 mmol/1, but is inhibited above that amount. The intrinsic fluorescence of the enzyme in E1 and E2 conformations is modified suggesting the occurrence of ascorbate-induced intermediate forms, distinct from those provoked by the addition of cations, magnesium and phosphate. These destabilized forms appear easier to be converted into catalytically active or increasingly inhibited states.     

Considerazioni sulla torulopsis pulcherrima

Zusammenfassung Aus Mosten von Umbrien und Toscana wurden 23 zu Torulopsis pulcherrima (Lindner) Saccardo gehörige Stämme isoliert, von denen 21 als die typische Art zu identifizieren waren (Rötung der Kultur nur bei Gegenwart von Eisen), während 2 Stämme, als var. rubra n. var. bezeichnet, sich außer durch andere Eigenschaften auch durch intensivere Farbstoffbildung unterscheiden.     

Ultrastructural complexity of nuclear components during early apoptotic phases in breast cancer cells.

Fractal morphometry was used to investigate the ultrastructural features of the plasma membrane, perinuclear membrane and nuclear chromatin in SK-BR-3 human breast cancer cells undergoing apoptosis. Cells were incubated with 1 microM calcimycin (A23187) for 24 h. Cells in the early stage of apoptosis had fractal dimension (FD) values indicating that their plasma membranes were less rough (lower FD) than those of control cells, while their perinuclear membranes were unaffected. Changes of the chromatin texture within the entire nucleus and in selected nuclear domains were more pronounced in treated cells. This confirms that the morphological reorganization imputable to a loss of structural complexity (reduced FD) occurs in the early stage of apoptosis, is accompanied by the inhibition of distinct enzymatic events and precedes the onset of conventional cellular markers, which can only be detected during the active phases of the apoptotic process.