Location of taste buds in intact taste papillae by a selective staining method.

Taste buds were found to stain strongly and selectively in intact papillae with highly acidic dyes such as ponceau S. In intact tongues the taste buds in the fungiform, circumvallate and foliate papillae of the cynomolgus monkey and in the fungiform papillae of the rat as well as the taste discs in the fungiform papillae of the frog could be visualized. This method enables a rapid location and counting of taste buds in taste papillae without preparing histological sections. In cynomolgus tongue material fixed in formalin, the dyes penetrate into the buds. In fresh tongues only the taste pore region of the buds stains, which suggests that in vivo taste buds are impenetrable underneath the pore.     

Short term thermal relief for improved fertility in dairy cattle during hot weather

Cooling dairy cows to basal body temperatures in a climatic-control barn before inseminating and for 1 to 6.5 days after, improved fertility during early summer in the hot desert climate of Arizona. The treatment was not as effective during later summer months when the animals had been exposed to high climatic temperature for a longer period. Physiological adaptation to heat stress, inimical to normal reproduction, is apparently irreversible over the short cooling period.Arizona Agricultural Experiment Station Technical Paper No 2622.     

Patterns and potential drivers of intraspecific variability in the body C,N, and P composition of a terrestrial consumer,the snowshoe hare (Lepus americanus)

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