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Acta Biotheoretica - Both physiological and evolutionary criteria of biological individuality are underpinned by the idea that an individual is a functionally integrated whole. However, a precise... 相似文献
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Carolina Torres Mariana Mimosa Leonardo Galetto 《Plant Systematics and Evolution》2013,299(8):1433-1441
Plant–pollinator interactions provide highly important ecological functions, and are influenced by floral nectar characteristics. The night blooming Datura ferox is an excellent model to test general hypotheses on the relationship between nectar traits (e.g., nectar secretion patterns, nectar chemical composition), pollinators and reproductive success for invasive, weedy species in highly modified ecosystems as crop fields. We hypothesized an adjustment between nectar composition and secretion dynamics through flower anthesis and the activity and requirements of nocturnal pollinators. Nectar chemical analyses showed low quantities of amino acids and lipids, phenolics, and alkaloids were not detected. D. ferox showed sucrose-dominant nectar with comparable amount of hexoses. Sugar proportions did not vary between populations or during flowering season. Most nectar is secreted before flower opening. Nectar resorption was detected at the end of anthesis. Experimentally drained flowers of both populations increased nectar production up to 50 % in the total amount of sugar per flower compared to control flowers. Nectar standing crop was relatively constant during the flowering season, but differences were detected between populations. Nectar traits of D. ferox would be favoring cross-pollination and maintaining seed production of this weed, since recently open flowers display a higher amount of nectar and they can renew nectar after a pollinator visit or reabsorb it at the end of anthesis. This nectar source may be important for native pollinators considering that human-induced forest fragmentation is related with the impoverishment of native flora from agro-ecosystems. 相似文献
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Keller Ricardo Ferreira Sobrinho Ana Carolina Monteiro Santini Carlos Leonardo Sacomani Marques Maicon Gonçalves Gabriel Eduardo de Moura Neto Luciane Aparecida Pascucci Sande de Souza 《Somatosensory & motor research》2013,30(3-4):199-203
AbstractBackground: Unilateral spatial neglect (USN) is the prevalent feature in patients with right-sided stroke. It is diagnosed through the behavior inattention test (BIT) and has a negative impact on patients affecting both their functional capacity and quality of life.Objective: Here, we aimed to evaluate the impact of USN on the quality of life of patients in the chronic phase of stroke.Methods: This is a cross-sectional study with stroke patients with USN. After confirming the presence of stroke through neuroimaging examinations and of USN through the BIT, patients’ quality of life was evaluated by using the EUROQOL scale. Spearman’s correlation was used to validate the correlation between patients’ USN and quality of life, with a p?<?.05 representing significant results.Results: Eighteen individuals were included. When correlating the value of each domain of the EUROQOL scale with the results of the BIT, we observed a negative correlation between mobility (r?=?–0.97; p?=?.000), self-care (r?=?–0.82; p?=?.013), usual activities (r?=?–0.87; p?=?.005); pain or discomfort (r?=?–0.88; p?=?.004), anxiety or depression (r?=?–0.97; p?=?.000), and EUROQOL total score (r?=?–0.97, p?=?.000).Conclusion: After a correlation between the overall EUROQOL and BIT scores, we suggest that the higher the USN degree is in stroke patients, the worse their perceived quality of life tends to be. 相似文献
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Danilo Roman-Campos Policarpo Sales-Júnior Hugo Leonardo Duarte Eneas Ricardo Gomes Silvia Guatimosim Catherine Ropert Ricardo Tostes Gazzinelli Jader Santos Cruz 《Memórias do Instituto Oswaldo Cruz》2013,108(2):243-245
Chagas disease, which is caused by the parasite Trypanosoma cruzi, is an important cause of heart failure. We investigated modifications in the cellular electrophysiological and calcium-handling characteristics of an infected mouse heart during the chronic phase of the disease. The patch-clamp technique was used to record action potentials (APs) and L-type Ca2+ and transient outward K+ currents. [Ca2+]i changes were determined using confocal microscopy. Infected ventricular cells showed prolonged APs, reduced transient outward K+ and L-type Ca2+ currents and reduced Ca2+ release from the sarcoplasmic reticulum. Thus, the chronic phase of Chagas disease is characterised by cardiomyocyte dysfunction, which could lead to heart failure. 相似文献
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Sergio A. Coelho-Souza Marcio R. Miranda Leonardo T. Salgado Ricardo Coutinho Jean R. D. Guimaraes 《Microbial ecology》2013,65(2):424-436
The ecological interaction between microorganisms and seaweeds depends on the production of secondary compounds that can influence microbial diversity in the water column and the composition of reef environments. We adapted the 3H-leucine incorporation technique to measure bacterial activity in biofilms associated with the blades of the macroalgae Sargassum spp. We evaluated (1) if the epiphytic bacteria on the blades were more active in detritus or in the biofilm, (2) substrate saturation and linearity of 3H-leucine incorporation, (3) the influence of specific metabolic inhibitors during 3H-leucine incorporation under the presence or absence of natural and artificial light, and (4) the efficiency of radiolabeled protein extraction. Scanning electron microscopy showed heterogeneous distribution of bacteria, diatoms, and polymeric extracellular secretions. Active bacteria were present in both biofilm and detritus on the blades. The highest 3H-leucine incorporation was obtained when incubating blades not colonized by macroepibionts. Incubations done under field conditions reported higher 3H-leucine incorporation than in the laboratory. Light quality and sampling manipulation seemed to be the main factors behind this difference. The use of specific metabolic inhibitors confirmed that bacteria are the main group incorporating 3H-leucine but their association with primary production suggested a symbiotic relationship between bacteria, diatoms, and the seaweed. 相似文献