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21.
Enzymic analysis of cyclic 3', 5'-AMP in mammalian tissues and urine 总被引:10,自引:0,他引:10
The details are presented for the analysis of 3′,5′ cyclic adenosine monophosphate (3′5′CAMP) in milligram amounts of mammalian tissues (muscles, liver, brain, and kidney) and in microliter samples of urine. An examination of the sources of difficulty and how they are effectively handled is also included. In the determination of tissue 3′5′CAMP the cyclic nucleotide is first separated from 5′-nucleoside mono-, di-, and triphosphates by cellulose thin-layer chromatography following Ba(OH)2-ZnSO4 precipitation of extracts. After quantitative recovery 3′,5′CAMP is converted to 5′ AMP and subsequently to ATP by the actions of phosphodiesterase, myokinase, and pyruvate kinase. Enzymic cycling with the hexokinase-pyruvate kinase system is then used to produce a proportional concentration of G-6-P equivalent to several thousand fold the ATP concentration and the G-6-P measured fluorometrically. Cyclic adenylate in urine samples is determined directly without prior separation from any urinary components. Examples are presented of the analytical procedures applied to the measurement of 3′5′CAMP levels in tissues and urine after various experimental treatments. These include the effects of epinephrine in skeletal muscle in vitro and in vivo, of adrenalectomy and hydrocortisone in liver, of ischemia in brain, and of hypertonic infusion on urinary excretion of 3′5′CAMP. 相似文献
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Beta interferon and oncostatin M activate Raf-1 and mitogen-activated protein kinase through a JAK1-dependent pathway. 总被引:7,自引:1,他引:6 下载免费PDF全文
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C Y Guo Y Wang D L Brautigan J M Larner 《The Journal of biological chemistry》1999,274(26):18715-18720
Ionizing radiation is known to activate multiple signal transduction pathways, but the targets of these pathways are poorly understood. Phosphorylation of histone H1 is thought to have a role in chromatin condensation/decondensation, and we asked whether ionizing radiation (IR) would alter H1 phosphorylation. Our data demonstrate that low doses of IR result in a dramatic, but transient, dephosphorylation of H1 isoforms. The in vivo IR-induced dephosphorylation of H1 is completely blocked by wortmannin and is abrogated in ataxia telangiectasia cells. Furthermore, we measured radiation-induced inhibition of cyclin dependent kinase activity and activation of histone H1 phosphatase activity. Both activities were affected by radiation-induced signals in an ATM-dependent manner. Thus, the rapid IR-induced dephosphorylation of H1 involves a pathway including ATM and a wortmannin-sensitive step leading to both inhibition of cyclin-dependent kinase activities as well as activation of H1 phosphatase(s). 相似文献
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Jun Mi Jaroslaw Dziegielewski Elzbieta Bolesta David L. Brautigan James M. Larner 《PloS one》2009,4(2)
DNA-dependent protein kinase (DNA-PK) plays a critical role in DNA damage repair, especially in non-homologous end-joining repair of double-strand breaks such as those formed by ionizing radiation (IR) in the course of radiation therapy. Regulation of DNA-PK involves multisite phosphorylation but this is incompletely understood and little is known about protein phosphatases relative to DNA-PK. Mass spectrometry analysis revealed that DNA-PK interacts with the protein phosphatase-6 (PP6) SAPS subunit PP6R1. PP6 is a heterotrimeric enzyme that consists of a catalytic subunit, plus one of three PP6 SAPS regulatory subunits and one of three ankyrin repeat subunits. Endogenous PP6R1 co-immunoprecipitated DNA-PK, and IR enhanced the amount of complex and promoted its import into the nucleus. In addition, siRNA knockdown of either PP6R1 or PP6 significantly decreased IR activation of DNA-PK, suggesting that PP6 activates DNA-PK by association and dephosphorylation. Knockdown of other phosphatases PP5 or PP1γ1 and subunits PP6R3 or ARS-A did not reduce IR activation of DNA-PK, demonstrating specificity for PP6R1. Finally, siRNA knockdown of PP6R1 or PP6 but not other phosphatases increased the sensitivity of glioblastoma cells to radiation-induced cell death to a level similar to DNA-PK deficient cells. Our data demonstrate that PP6 associates with and activates DNA-PK in response to ionizing radiation. Therefore, the PP6/PP6R1 phosphatase is a potential molecular target for radiation sensitization by chemical inhibition. 相似文献
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Heidi K. Ortmeyer Joseph Larner Barbara C. Hansen 《Obesity (Silver Spring, Md.)》1995,3(Z4):605S-608S
We have previously demonstrated that D-chiroinositol, administered intravenously to insulin-resistant monkeys, increases the rate of disappearance of plasma glucose and insulin. The purpose of the present study was to determine whether orally administered D-chiroinositol might also similarly improve the postprandial plasma glucose profile of hyperinsulinemic insulin-resistant monkeys. A complete liquid diet meal (15 ml/kg body weight) was ingested by each of six monkeys on two occasions separated by 10 days, with conditions identical except D-chiroinositol (500 mg/kg body weight) was added to the second meal. At 110 minutes following each meal, the monkeys were anesthetized and blood samples obtained at 120, 150, 180, 210, 240, 270 and 300 minutes. Plasma glucose and insulin concentrations were determined. The mean plasma glucose concentration (120 ? 300 minutes) was significantly lower after the meal containing D-chiroinositol compared to the control meal (7.1 ± 1.2 vs. 7.8 ± 1.2 mM) (p<0.05). Plasma insulin concentrations tended to be lower after the meal containing D-chiroinositol compared to the control meal (3930 ± 1068 vs. 4518 ± 1200 pM) (p<0.15, ns). We conclude that in hyperinsulinemic monkeys, D-chiroinositol added to a meal lowers postprandial plasma glucose without an increase in plasma insulin, and therefore may be a useful agent for reducing meal-induced hyperglycemia without inducing hyperinsulinemia. 相似文献
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