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101.
Amphioxus and the Utility of Molecular Genetic Data for Hypothesizing Body Part Homologies between Distantly Related Animals 总被引:1,自引:0,他引:1
Expression domains of developmental genes can indicate bodypart homologies between distantly related animals and give insightsinto interesting evolutionary questions. Two of the chief criteriafor recognizing homologies are relative position with respectto surrounding body parts and special quality (for instance,a vertebrate testis, regardless of its location, is recognizableby its seminiferous cysts or tubules). When overall body plansof two animals are relatively similar, as for amphioxus versusvertebrates, body part homologies can be supported by developmentalgene expression domains, which have properties of special qualityand relative position. With expression patterns of AmphiNk2-land AmphiPax2/5/8, we reexamine the proposed homology betweenthe amphioxus endostyle and the vertebrate thyroid gland, anda previously good homology is made better. When body plans ofanimals are disparate, body part homologies supported by moleculargenetic data are less convincing, because the criterion of relativeposition of gene expression domains becomes uncertain. Thus,when expression of amphioxus AmphiBMP2/4 is used to comparethe dorsoventral axis between amphioxus and other animals, acomparison between amphioxus and vertebrates is more convincingthan comparison between amphioxus and other invertebrates withdisparate body plans. In spite of this difficulty, the use ofdevelopmental genetic evidence comparing animals with disparatebody plans is currently putting the big picture of evolutioninto new perspective. For example, some molecular geneticistsare now suggesting that the last common ancestor of all bilateriananimals might have been more annelid-like than flatworm-like. 相似文献
102.
LINDA F. CHAPMAN VINCENT P. CIRILLO THEODORE L. JAHN 《The Journal of eukaryotic microbiology》1965,12(1):47-51
SYNOPSIS. Whole cells of Polytoma obtusum do not utilize glucose or other hexoses, but cell-free extracts show glucokinase activity having a pH optimum at 8.5 and a Q10 of 2.4 between 0–20°C. Lack of glucom utilization by whole cells reflects a permeability barrier to all sugars.
To determine whether inability of acetate-grown cells to utilize butyrate also denoted a permeability barrier, permeability to acetate and butyrate was studied in acetate- and butyrate-grown cells However, acetate-grown cells accumulated C-14 labeled butyrate against a concentration gradient. Therefore inability of acetate-grown cells to utilize butyrate does not denote a permeability barrier.
Acetate-kinase activity was consktently demonstrated in cell-free extracts of both acetate- and butyrate-grown cells. Butyrate kinase was consistently found only in butyrate-grown cells. However, the absence of buttymte kinase in acetate-grown cells cannot be claimed since occasionally butyrate kinase activity was demonstrated in extracts of acetate-grown cells. 相似文献
To determine whether inability of acetate-grown cells to utilize butyrate also denoted a permeability barrier, permeability to acetate and butyrate was studied in acetate- and butyrate-grown cells However, acetate-grown cells accumulated C-14 labeled butyrate against a concentration gradient. Therefore inability of acetate-grown cells to utilize butyrate does not denote a permeability barrier.
Acetate-kinase activity was consktently demonstrated in cell-free extracts of both acetate- and butyrate-grown cells. Butyrate kinase was consistently found only in butyrate-grown cells. However, the absence of buttymte kinase in acetate-grown cells cannot be claimed since occasionally butyrate kinase activity was demonstrated in extracts of acetate-grown cells. 相似文献
103.
TIMOTHY G. GEARY ALAN A. DIVO LINDA C. BONANNI JAMES B. JENSEN 《The Journal of eukaryotic microbiology》1985,32(4):608-613
In a semi-defined minimal medium for cultivation of Plasmodium falciparum, ribose, mannose, fructose, galactose, and maltose could not replace glucose. Hypoxanthine was the preferred purine source for the parasite over adenine, guanine, inosine, adenosine and guanosine although all supported growth equally. Inhibitors of nucleoside uptake had low potency in killing the parasites but depressed incorporation of [3H]adenosine more than [3H]hypoxanthine. Glutamate could not be replaced by 5-oxoproline, indicating that the γ-glutamyl transferase pathway for amino acid uptake is probably not found in this organism. Adenine, nicotinamide, and orotic acid could not supplement glutamine-deficient medium. The pyridoxine antagonists isoniazid and 4-deoxypyridoxine were reversed by amino acid supplementation, suggesting that transaminases may be targets of these drugs. Orotic acid, but not glutathione or its amino acid components, partially reversed the effects of 8-methylamino-8-desmethyl riboflavin. Thus, the flavin enzyme, dihydroorotic acid dehydrogenase, but not glutathione reductase, appears to be a target of this riboflavin antagonist. Five biotin antagonists had no significant activity. The choline antagonist 2-(tert-butylamino)ethanol and thiamin uptake inhibitors had nonspecific inhibitory effects, which were not reversed by the respective target vitamin. Buthionine sulfoximine and methionine sulfoximine, inhibitors of glutathione synthesis, had significant oxygen-dependent toxicity. Six sulfonamides showed marked variation in potency and efficacy. Sulfathiazole and sulfadoxine were reversed differentially by p-aminobenzoic acid, folic acid, and folinic acid. Folinic acid was more effective than folic acid at reversing the toxicity of the dihydrofolate reductase inhibitors aminopterin and pyrimethamine; p-aminobenzoic acid had no effect. 相似文献
104.
IAN W. B. THORNTON DAVID RUNCIMAN SIMON COOK LINDA F. LUMSDEN TUKIRIN PARTOMIHARDJO NATASHA K. SCHEDVID JUNICHI YUKAWA SEAMUS A. WARD 《Biological journal of the Linnean Society. Linnean Society of London》2002,77(3):275-317
In theory, one factor determining the rate and nature of the assembly of island biotas is the presence or absence of stepping stone islands, yet no field studies have demonstrated stepping stone function in practice. Krakatau, in Sunda Strait, is about equidistant from Java and Sumatra. Sebesi lies about half way between Krakatau and Sumatra, but no island intervenes between Krakatau and the nearest coast of Java. We assess the evidence that Sebesi has acted as an important stepping stone for Krakatau's recolonization since the devastating 1883 volcanic eruption. About a quarter of Krakatau's resident land birds, two-fifths of its reptiles, bats and land molluscs, and about two-thirds of its termites, pteridophytes, butterflies and spermatophytes are unknown on Sebesi, evidently having colonized without stepping stone involvement. Identifiable Sumatran taxa do not outnumber identifiable Javan ones on Krakatau, nor do historical distribution records indicate movement from Sebesi to Krakatau in animal groups. Krakatau's biota is not a subset of Sebesi's in predominantly anemochorous or thallassochorous plant groups, butterflies, reptiles or bats, and is only marginally so in termites. It is a subset in predominantly zoochorous spermatophyte groups, except Ficus species, and in birds and land molluscs. Comparison with a weaker stepping stone candidate, Panaitan, provides no evidence for a stepping stone role for Sebesi in butterflies or termites. We discuss the dispersal and establishment constraints on colonization by the groups involved, and conclude that, overall, Sebesi had little impact as a stepping stone. Instead, it is more probable that divergence of the environments of the two islands has led to an increasingly independent recolonization of Krakatau. © 2002 The Linnean Society of London, Biological Journal of the Linnean Society , 2002, 77 , 275–317. 相似文献
105.
Evolution of pipoid frogs:intergeneric relationships of the aquatic frog family Pipidae (Anura) 总被引:1,自引:0,他引:1
The 27 species of the aquatic frog family Pipidae are currently arranged in four genera: Xenopus (15 species), Hymenochirus (four species), and the poorly known genus Pseudhymenochirus (one species) occur in Africa; Pipa (seven species) is found in South America and lower Central America. Despite extensive work on the biology of Xenopus from various disciplines, the evolutionary relationships of Xenopus to other pipids have not been resolved. Phylogenetic analyis of morphological features of pipid frogs indicates that, contrary to earlier opinions, Hymenochirus and Pipa are closest relatives (sister-groups); these genera are placed in the subfamily Pipinae. Also, the currently recognized species of Xenopus do not form a natural group; the species tropicalis and epitropicalis are more closely related to Hymenochirus + Pipa than to the remaining species of Xenopus . The two discordant species are transferred to the genus Silurana , which is relegated to the new subfamily Siluraninae; it is the sister-group of the Pipinae. The remaining species of Xenopus constitute a monophyletic group that is placed in the subfamily Xenopodinae as the sister-group of the other genera of pipids. 相似文献
106.
Abstract The capacity to self‐select an optimal balance of macronutrients (protein and carbohydrate) is studied in two populations of Melanoplus sanguinipes F. (Orthoptera: Acrididae). One population derives from the subarctic (interior of Alaska) and the other from the temperate zone (Idaho, U.S.A.). Over the duration of the fourth and fifth stadia, Alaskan grasshoppers consistently self‐select a diet centred on a 0.90 ratio of protein : carbohydrate, whereas protein and carbohydrate intake by the Idaho grasshoppers is contingent on the particular food choices presented to them. When restricted to imbalanced diets, the Alaskan grasshoppers develop more rapidly than the Idaho grasshoppers, regardless of diet composition. The Idaho grasshoppers also have a greater amount of lipid than the Alaskan grasshoppers across all diets. Performance measures (body mass, survival, developmental times) are more sensitive to dietary imbalances in the Alaskan grasshoppers than in the Idaho grasshoppers. When fed diets with low, but balanced, proportions of protein and carbohydrate, grasshoppers of both populations are able to increase consumption to compensate for the low concentration of nutrients. The results suggest that demographic responses of insects to changes in host plant quality, such as may result from climate change, may differ among populations within a species. 相似文献
107.
ERIC C. YIP DAVID M. ROWELL LINDA S. RAYOR 《Biological journal of the Linnean Society. Linnean Society of London》2012,106(4):749-762
Movement among social groups interacts with the costs and benefits of group‐living in complex ways. Unlike most other social spiders, the social huntsman spider, Delena cancerides, appears to enter foreign colonies, discriminates kin from non‐kin, and has very limited dispersal options because their bark retreats are rare, making this species an interesting model organism with which to examine the role of inter‐colony movement on group‐living. We examined movement among field colonies of D. cancerides in three ways: (1) by tracking the dispersal and immigration of marked spiders into foreign colonies; (2) by recording resident spiders' behaviour toward introduced immigrants; and (3) by inferring intra‐colony relatedness and immigration patterns through allozyme electrophoresis. Of the marked spiders, only young juveniles moved into neighbouring colonies, whereas subadults and adults did not. Introduced juveniles were tolerated in foreign colonies, whereas introduced adult males and subadults were usually attacked by the resident adult female, unless she had similar sized subadult/adult offspring of her own. Allozyme profiles from unmanipulated field colonies showed that 47% of sampled colonies contained at least one immigrant and that average within colony relatedness was below 0.5. These data align with previous research on the costs and benefits of group‐living for D. cancerides, suggesting that spiders actively seek and regulate group membership based on interests of both the immigrant and the colony. © 2012 The Linnean Society of London, Biological Journal of the Linnean Society, 2012, ?? , ??–??. 相似文献
108.
TRANSPORT PROCESSES AND WATER RELATIONS 总被引:4,自引:3,他引:1
109.
LINDA L. PIFER 《The Journal of eukaryotic microbiology》1989,36(1):23S-24S
The in vitro cultivation of Pneumocystis carinii in chick lung cell culture made it possible to observe the organism proceeding through its life cycle It provided the foundation for extensive scrocpidcmiologic studies, for in vitro drug screening, and for essential biological, metabolic, and morphologic research, in vitro culture made possible the discovery of P. carinii antigencmia, its biochemical nature, and its relevance to subclinical and clinical infection. Its utility in the presumptive diagnosis of P. carinii pneumonia and in monitoring responses to drug therapy illustrate the value and clinical application of basic research. 相似文献
110.
The lectin, Concanavalin A (Con A), inhibits cell pairing during mating in Tetrahymena and binds to the surface of pairing cells via receptors concentrated around the conjugation junction. Concanavalin A is also ingested in large amounts into food vacuoles. To dispel the possibility that Con A inhibits pairing via uptake into food vacuoles or through induction of food vacuole formation and to strengthen the idea that pairing is blocked through binding of Con A to cell surface receptors, we have conducted three types of experiments: 1) attempts to inhibit pairing by feeding with nutrients and with tantalum, a non-nutritive reagent; 2) a temporal analysis of the presence of food vacuoles in mating cells fed with tantalum; and 3) analysis of the restoration of pairing following the addition of α-methyl mannoside to cells previously treated with inhibitory concentrations of Con A. The results of these studies support the idea that Con A inhibits pairing by binding to receptors located on the cell surface and not by induction of or uptake into food vacuoles. We also present evidence that cells grown in an enriched proteose peptone medium are able to pair and undergo morphogenesis more readily than cells grown in 2% proteose peptone. 相似文献