Morphological changes in the small intestine of rats after its temporary exclusion from blood circulation]

The experiments were performed in 56 albino rats (15of them served as a control. The small intestine was excluded from the blood circulation for 45minutes and then pieces of the proximal and distal parts of the intestine were taken forhistological and histochemical study. It was shown that such a term of anemia resulted indeep but reversible changes in the mucous sheath and in the intramural nervous apparatus of thesmall intestine. The state of the organ wall became normal in 15 days after operation.     

The effect of epidermal growth factor on protein phosphorylation in normal and transformed hepatocytes

Qualitative differences in the content of tyrosine-phosphorylated proteins in normal and transformed hepatocytes have been found using the method of two-dimensional electrophoresis. Epidermal growth factor (EGF) has induced quantitative changes in the spectra of phosphotyrosine-containing proteins in normal cells and qualitative changes in the transformed ones. Results of immunoprecipitation with antibodies against phosphotyrosine permit revealing a protein with Mm 50 kDa which is subjected to EGF-dependent tyrosine-phosphorylation in normal hepatocytes. The intensity of protein phosphorylation is 10 times higher in the transformed cells but the dependence of this process on the EGF is not exhibited. The role of protein-tyrosine-kinases in the transmission of a mitogenic signal and in liver carcinogenesis is discussed.