全文获取类型
收费全文 | 1067篇 |
免费 | 100篇 |
出版年
2021年 | 9篇 |
2020年 | 7篇 |
2019年 | 10篇 |
2018年 | 10篇 |
2017年 | 14篇 |
2016年 | 28篇 |
2015年 | 41篇 |
2014年 | 45篇 |
2013年 | 62篇 |
2012年 | 67篇 |
2011年 | 67篇 |
2010年 | 52篇 |
2009年 | 52篇 |
2008年 | 57篇 |
2007年 | 39篇 |
2006年 | 39篇 |
2005年 | 40篇 |
2004年 | 36篇 |
2003年 | 28篇 |
2002年 | 32篇 |
2001年 | 25篇 |
2000年 | 33篇 |
1999年 | 21篇 |
1998年 | 20篇 |
1997年 | 25篇 |
1996年 | 18篇 |
1995年 | 25篇 |
1994年 | 23篇 |
1993年 | 21篇 |
1992年 | 21篇 |
1991年 | 16篇 |
1990年 | 11篇 |
1989年 | 13篇 |
1988年 | 17篇 |
1987年 | 9篇 |
1986年 | 10篇 |
1985年 | 6篇 |
1984年 | 12篇 |
1983年 | 9篇 |
1982年 | 21篇 |
1981年 | 5篇 |
1980年 | 4篇 |
1979年 | 8篇 |
1978年 | 5篇 |
1977年 | 8篇 |
1976年 | 9篇 |
1975年 | 5篇 |
1973年 | 6篇 |
1972年 | 4篇 |
1958年 | 3篇 |
排序方式: 共有1167条查询结果,搜索用时 31 毫秒
101.
Stanislav Isayenkov Jean-Charles Isner Frans JM Maathuis 《Plant signaling & behavior》2011,6(8):1201-1204
Potassium (K) is one of the major nutrients that is essential for plant growth and development. The majority of cellular K+ resides in the vacuole and tonoplast K+ channels of the TPK (Two Pore K) family are main players in cellular K+ homeostasis. All TPK channels were previously reported to be expressed in the tonoplast of the large central lytic vacuole (LV) except for one isoform in Arabidopsis that resides in the plasma membrane. However, plant cells often contain more than one type of vacuole that coexist in the same cell. We recently showed that two TPK isoforms (OsTPKa and OsTPKb) from Oryza sativa localize to different vacuoles with OsTPKa predominantly found in the LV tonoplast and OsTPKb primarily in smaller compartments that resemble small vacuoles (SVs). Our study further revealed that it is the C-terminal domain that determines differential targeting of OsTPKa and OsTPKb. Three C-terminal amino acids were particularly relevant for targeting TPKs to their respective endomembranes. In this addendum we further evaluate how the different localization of TPKa and TPKb impact on their physiological role and how TPKs provide a potential tool to study the physiology of different types of vacuole.Key words: TPK channels, small vacuoles, vacuolar targeting, potassiumThe roles of plant vacuolar K+ channels are diverse and include potassium homeostasis, turgor regulation and responses to abiotic stress. Vacuolar K+-selective channels belong to two-pore K+ (TPK) channel families which have been found in genomes of many plant species such as Arabidopsis, poplar, Physcomitrella, Eucalyptus, barley, potato, rice and tobacco (Fig. 1). TPKs have structural similarity to mammalian “tandem P domain” channels with a secondary structure that contains four transmembrane domains and two pore regions (Fig. 2).1–5 TPK channels have pore regions with a GYGD signature that endows K+ selectivity and a variable number of Ca2+ binding EF domains in the C terminus.3–8 One of the best characterized members of the TPK family is AtTPK1 from Arabidopsis thaliana. AtTPK1 activity is voltage independent but sensitive to cytosolic Ca2+, cytosolic pH and N-terminal phosphorylation by 14-3-3 proteins.5,6,8,9 In Arabidopsis, AtTPK1 expresses in the large lytic vacuole (LV) and plays roles in cellular K+ homeostasis, K+-release during stomatal closure and seed germination.4,5 Other members of the Arabidopsis TPK family (AtTPK2, AtTPK3, AtTPK5) have been shown to localize to the LV but also showed some expression in smaller, vesicle-like, compartments.4 However, none of these isoforms appears to form functional channels in planta although our experiments with heterologous expression of AtTPK3 and AtTPK5 in the K+ uptake deficient E. coli LB2003 demonstrates complementation of bacterial growth phenotype (Isayenkov S, et al. unpublished results). Equally intriguing, is the plasma membrane localization of the Arabidopsis TPK4 isoform, in spite of its sequence being very similar to that of other TPKs.10Open in a separate windowFigure 1Phylogenetic tree of plant TPKs. The three main clusters of TPKs comprise: Cluster 1 with AtTPK1-like channels; Cluster 2 with AtTPK3/TPK5-like channels; Cluster 3 with barley HvTPKb. Bootstrap analysis was performed using ‘Molecular Evolutionary Genetics Analysis, MEGA4’ software available at www.megasoftware.net/mega4/megaOpen in a separate windowFigure 2Two-pore potassium channel secondary structure. TPK channels comprise four transmembrane domains (1–4) and two pore regions (P) per subunit. Functional channels are formed from two subunits. In most TPKs, both P regions contain a K+ selectivity signature, GYGD. However, the tobacco NtTPKa isoform has different motifs in the second P domain. In the N terminal region, TPKs have a 14-3-3 binding domain that impact on channel activity, with the binding of 14-3-3 protein leading to channel activation. C-termini of TPKs show a varying number of putative Ca2+ binding “EF hands” which may vary from zero to two. 相似文献
102.
Antoine Kremer Barbara Vinceti Ricardo Alia Jaroslav Burczyk Stephen Cavers Bernd Degen Reiner Finkeldey Silvia Fluch Dusan G?m?ry Felix Gugerli Hans Peter Koelewijn Jarkko Koskela Fran?ois Lef��vre Michele Morgante Gerhard Mueller-Starck Christophe Plomion Gail Taylor Jozef Turok Outi Savolainen Birgit Ziegenhagen 《Tree Genetics & Genomes》2011,7(4):869-875
This article is a summary report of the international conference "Forest ecosystem genomics and adaptation" organized by the EVOLTREE Network of Excellence in San Lorenzo de El Escorial (Madrid), Spain, from 9 to 11 June 2010. Main achievements and results of the network are presented for the eight thematic sessions and a stakeholder session. The conference has shown that adaptive responses of trees to biotic or abiotic selection pressures can now be investigated at the gene level for traits of adaptive significance. Candidate genes have been catalogued for phenological and drought-related traits in important tree families (Salicaceae, Fagaceaea and Pinaceae), and their variation in natural populations is being explored. Genomics can now be integrated in ecological research to investigate evolutionary response to climate changes in a wide range of species. New avenues of research were also highlighted as the exploration of gene networks involved in adaptive responses and the combination of experimental and modelling approaches to disentangle components of evolutionary changes triggered by climate change. The main focus of the conference was the adaptation of trees to environmental changes. The conference was organized in eight thematic sessions ranging from genomic approaches aiming at identifying genes of adaptive significance to practical issues regarding mitigation options for combating climate change. A dialogue between scientists and end users took place in the form of an ad hoc stakeholder session. A panel of end users from various forest and policy-making institutions expressed their expectations, and the discussions with the scientists addressed the potential applications of research findings to the management of genetic resources in the context of climate changes. The conference was introduced by two keynote speakers Dr. Pierre Mathy from the European Commission, Directorate General of Research, and Dr. Allen Solomon, former National Program Leader for Global Change, US Forest Service. All the thematic sessions were introduced by high-level invited speakers from the respective fields. 相似文献
103.
104.
Mestre O Luo T Dos Vultos T Kremer K Murray A Namouchi A Jackson C Rauzier J Bifani P Warren R Rasolofo V Mei J Gao Q Gicquel B 《PloS one》2011,6(1):e16020
Background
The Beijing family is a successful group of M. tuberculosis strains, often associated with drug resistance and widely distributed throughout the world. Polymorphic genetic markers have been used to type particular M. tuberculosis strains. We recently identified a group of polymorphic DNA repair replication and recombination (3R) genes. It was shown that evolution of M. tuberculosis complex strains can be studied using 3R SNPs and a high-resolution tool for strain discrimination was developed. Here we investigated the genetic diversity and propose a phylogeny for Beijing strains by analyzing polymorphisms in 3R genes.Methodology/Principal Findings
A group of 3R genes was sequenced in a collection of Beijing strains from different geographic origins. Sequence analysis and comparison with the ones of non-Beijing strains identified several SNPs. These SNPs were used to type a larger collection of Beijing strains and allowed identification of 26 different sequence types for which a phylogeny was constructed. Phylogenetic relationships established by sequence types were in agreement with evolutionary pathways suggested by other genetic markers, such as Large Sequence Polymorphisms (LSPs). A recent Beijing genotype (Bmyc10), which included 60% of strains from distinct parts of the world, appeared to be predominant.Conclusions/Significance
We found SNPs in 3R genes associated with the Beijing family, which enabled discrimination of different groups and the proposal of a phylogeny. The Beijing family can be divided into different groups characterized by particular genetic polymorphisms that may reflect pathogenic features. These SNPs are new, potential genetic markers that may contribute to better understand the success of the Beijing family. 相似文献105.
Large-scale candidate gene analysis of HDL particle features 总被引:1,自引:0,他引:1
Kaess BM Tomaszewski M Braund PS Stark K Rafelt S Fischer M Hardwick R Nelson CP Debiec R Huber F Kremer W Kalbitzer HR Rose LM Chasman DI Hopewell J Clarke R Burton PR Tobin MD Hengstenberg C Samani NJ 《PloS one》2011,6(1):e14529
Background
HDL cholesterol (HDL-C) is an established marker of cardiovascular risk with significant genetic determination. However, HDL particles are not homogenous, and refined HDL phenotyping may improve insight into regulation of HDL metabolism. We therefore assessed HDL particles by NMR spectroscopy and conducted a large-scale candidate gene association analysis.Methodology/Principal Findings
We measured plasma HDL-C and determined mean HDL particle size and particle number by NMR spectroscopy in 2024 individuals from 512 British Caucasian families. Genotypes were 49,094 SNPs in >2,100 cardiometabolic candidate genes/loci as represented on the HumanCVD BeadChip version 2. False discovery rates (FDR) were calculated to account for multiple testing. Analyses on classical HDL-C revealed significant associations (FDR<0.05) only for CETP (cholesteryl ester transfer protein; lead SNP rs3764261: p = 5.6*10−15) and SGCD (sarcoglycan delta; rs6877118: p = 8.6*10−6). In contrast, analysis with HDL mean particle size yielded additional associations in LIPC (hepatic lipase; rs261332: p = 6.1*10−9), PLTP (phospholipid transfer protein, rs4810479: p = 1.7*10−8) and FBLN5 (fibulin-5; rs2246416: p = 6.2*10−6). The associations of SGCD and Fibulin-5 with HDL particle size could not be replicated in PROCARDIS (n = 3,078) and/or the Women''s Genome Health Study (n = 23,170).Conclusions
We show that refined HDL phenotyping by NMR spectroscopy can detect known genes of HDL metabolism better than analyses on HDL-C. 相似文献106.
Background
New diagnosis tests are urgently needed to address the global tuberculosis (TB) burden and to improve control programs especially in resource-limited settings. An effective in vitro diagnostic of TB based on serological methods would be regarded as an attractive progress because immunoassays are simple, rapid, inexpensive, and may offer the possibility to detect cases missed by standard sputum smear microscopy. However, currently available serology tests for TB are highly variable in sensitivity and specificity. Lipolytic enzymes have recently emerged as key factors in lipid metabolization during dormancy and/or exit of the non-replicating growth phase, a prerequisite step of TB reactivation. The focus of this study was to analyze and compare the potential of four Mycobacterium tuberculosis lipolytic enzymes (LipY, Rv0183, Rv1984c and Rv3452) as new markers in the serodiagnosis of active TB.Methods
Recombinant proteins were produced and used in optimized ELISA aimed to detect IgG and IgM serum antibodies against the four lipolytic enzymes. The capacity of the assays to identify infection was evaluated in patients with either active TB or latent TB and compared with two distinct control groups consisting of BCG-vaccinated blood donors and hospitalized non-TB individuals.Results
A robust humoral response was detected in patients with active TB whereas antibodies against lipolytic enzymes were infrequently detected in either uninfected groups or in subjects with latent infection. High specifity levels, ranging from 93.9% to 97.5%, were obtained for all four antigens with sensitivity values ranging from 73.4% to 90.5%, with Rv3452 displaying the highest performances. Patients with active TB usually exhibited strong IgG responses but poor IgM responses.Conclusion
These results clearly indicate that the lipolytic enzymes tested are strongly immunogenic allowing to distinguish active from latent TB infections. They appear as potent biomarkers providing high sensitivity and specificity levels for the immunodiagnosis of active TB. 相似文献107.
108.
109.
110.
The biochemical regulation of photosynthate partitioning was investigated in a starchless mutant (TC7) of Arabidopsts thaliana (L.) Henyh, that was deficient in chloroplast phosphoglucomutase (Caspar et al. 1985. Plant Physiol. 79: 11–17). Plants were raised at 20°C with a 20 h light and 4 h dark period, so that the growth rates of the mutant and wild type were similar. Two or 3 isoforms of phosphoglucomutase were separated by ion-exchange chromatography using mutant and wild type leaf preparations, respectively. Initial rate kinetics of all isoforms were similar. Light-saturated photosynthetic oxygen evolution rates of the mutant and wild type were 224 and 302 nmol g-1 chlorophyll h-1 , respectively. Starch, sucrose and hexose concentrations were unchanged in wild type leaves after a dark to light transition, whereas sucrose and hexose increased in mutant leaves. Hexose-phosphates accumulated in both genotypes in the light, although the steady-state leaf concentrations of glucose 6-phosphate were 3-fold higher in mutant than in wild type samples. Fructose 2,6-bisphosphate and glucose 1,6-bisphosphate were lower in the mutant than in the wild type at the end of the dark period when mutant leaves were depleted of carbohydrates. Levels of UTP were lower in the mutant than in the wild type, possibly indicating that growth conditions had induced phosphate limited photosynthesis. These results are discussed in relation to the regulation of photosynthetic carbon metabolism. 相似文献