Seed preference and energy intake of Goldfinches Carduelis carduelis in the breeding season

The food of a population of Europeans Goldfinches breeding in Orchards was studied. More than 5% of the total foodstuff consumed was met by the seeds of seven food plants. Water, lipid, carbohydrate and protein content was determined from 20 different plants which Goldfinches used during the breeding season in order to calculate energy content. The energy of fresh seeds varied from 1.88 kJ/g in Tussilago farfara to 12.14 kJ/g in Dactylis glomerata. The dependence of the rate of energy intake (profitability) on the pecking rate and the size (weight) of the seeds was calculated. The profitability of various seeds is in agreement with the relative number of birds using them. Calculation of the feeding time that a Goldfinch needs during daylight hours, if it feeds only on a particular plant, indicates that there are several foods that cannot fill the energy demands of the bird during the breeding season. These are eaten only if others with a high energy content are not available. Thus food selection in Goldfinches depends, apart from availability, on seed profitability. The oxygen consumption of a male Goldfinch was significantly higher when the animal was feeding on seed heads of Tussilago farfara and Taraxacum officinale than when it fed on loose dry seeds, and so, different feeding techniques have to be taken into account in calculating net energy gain.     

Molecular phylogeny of the Raphidiidae (Raphidioptera)*

We present a molecular phylogeny of the family Raphidiidae including representatives of 21 of the 26 genera. Sequences from the nuclear gene for the large subunit ribosomal RNA (28S rRNA) and the mitochondrial cytochrome c oxidase subunit 3 gene (cox3) were used. For the phylogenetic reconstructions we applied automated and manual approaches for sequence alignment and different evolutionary models and tree building algorithms. The trees based on the two alignment approaches were rather similar in their overall topology. A combination of both marker sequences increased the resolution of the trees. The six clades within the raphidiid family that emerged represent either single genera or groups of genera, namely: (i) the Nearctic genus Agulla Navás, (ii) the Nearctic/Central American genus Alena Navás, (iii) the Central Asiatic and Eastern Palaearctic genus Mongoloraphidia H. Aspöck & U. Aspöck, (iv) the Palaearctic Puncha clade, (v) the western Mediterranean Ohmella clade, and (vi) the Palaearctic Phaeostigma clade. The New World taxa Agulla and Alena are placed as successive out‐groups to a monophyletic Palaearctic clade. The Mongoloraphidia clade is distributed in the eastern Palearctic while the remaining three clades are exclusively (Ohmella clade) or mainly distributed in the western Palaearctic. The early radiation of extant Raphidiidae is interpreted based on the phylogenetic tree obtained in the present study, and the geological and palaeobiological processes around the K–T boundary.     

Chicken major histocompatibility complex congenic lines differ in the percentages of lymphocytes bearing CD4 and CD8 antigens

In the experiments to be described two congenic inbred lines CB and CC and two recombinant lines CB.R1 and CC.R1 were used. All four lines differ only in regard to the major histocompatibility complex (MHC). To determine the percentage distributions of the two cell subsets in peripheral blood lymphocytes (PBL) in these lines, monoclonal antibodies to these two antigens were used. By FACScan there were more CD4+PBL in CB and CB.R1 lines (share B-F/B-L region, controlling class I/class II antigens with line CB) than CC and CC.R1, while the reverse was true with CD8+ subsets. There were more CD8+ PBL in the CC and CC.R1 lines and less in CB and CB.R1 lines. The ratio of CD4+ to CD8+ in CB chickens was 3.4 +/- 0.2 and in CC chickens 1.6 +/- 0.1.