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951.
952.
Kimiko Hirayama Shota Kawamura Tatsuya Nishimura Hikaru Takahara 《Journal of plant research》2010,123(5):655-663
Recently, populations of Castanopsis cuspidata have often expanded into secondary forests in western Japan. To determine the establishment processes of this species, we
analyzed its spatial distribution in a secondary forest dominated by Quercus variabilis and Quercus serrata that is located adjacent to a stand dominated by C. cuspidata. Saplings, defined as ≥30 cm stem length (SL) and <5 cm diameter at breast height (DBH), were abundant and their size distribution
was inversely J-shaped, indicating continuous recruitment. Although seedlings (SL < 30 cm) and small saplings (30 ≤ SL < 50 cm)
of C. cuspidata were aggregated near flowering trees of this species, some were found ≥40 m from the nearest adults, suggesting that there
is animal-aided dispersal of acorns. The distribution of larger-sized individuals (≥100 cm SL) of C. cuspidata was unrelated to distance from the nearest flowering C. cuspidata or dominant Quercus species (≥5 cm DBH), but was associated with dead Pinus densiflora trees, which were abundant at the site. Thus, the establishment of C. cuspidata in this forest is controlled mainly by two factors, viz. patterns of acorn dispersal by animals, and forest disturbance regime
(i.e., deaths of pine trees). 相似文献
953.
Yoshiki Nishimura 《Journal of plant research》2010,123(2):149-162
An intriguing feature of most eukaryotes is that chloroplast (cp) and mitochondrial (mt) genomes are inherited almost exclusively
from one parent. Uniparental inheritance of cp/mt genomes was long thought to be a passive outcome, based on the fact that
eggs contain multiple numbers of organelles, while male gametes contribute, at best, only a few cp/mtDNA. However, the process
is likely to be more dynamic because uniparental inheritance occurs in organisms that produce gametes of identical sizes (isogamous).
In Chlamydomonas reinhardtii, the uniparental inheritance of cp/mt genomes is achieved by a series of mating type-controlled events that actively eliminate
the mating type minus (mt−) cpDNA. The method by which Chlamydomonas selectively degrades mt− cpDNA has long fascinated researchers, and is the subject of this review. 相似文献
954.
The unusual basic amino acid, hypusine [Nε-(4-amino-2-hydroxybutyl)-lysine], is a modified lysine with the addition of the 4-aminobutyl moiety from the polyamine spermidine.
This naturally occurring amino acid is a product of a unique posttranslational modification that occurs in only one cellular
protein, eukaryotic translation initiation factor 5A (eIF5A, eIF-5A). Hypusine is synthesized exclusively in this protein
by two sequential enzymatic steps involving deoxyhypusine synthase (DHS) and deoxyhypusine hydroxylase (DOHH). The deoxyhypusine/hypusine
synthetic pathway has evolved in archaea and eukaryotes, and eIF5A, DHS and DOHH are highly conserved suggesting a vital cellular
function of eIF5A. Gene disruption and mutation studies in yeast and higher eukaryotes have provided valuable information
on the essential nature of eIF5A and the deoxyhypusine/hypusine modification in cell growth and in protein synthesis. In view
of the extraordinary specificity and functional significance of hypusine-containing eIF5A in mammalian cell proliferation,
eIF5A and the hypusine biosynthetic enzymes are novel potential targets for intervention in aberrant cell proliferation. 相似文献
955.
956.
Kyosuke Nakata Yumiko Ishikawa Yasuhiro Funada Richard S. Blumberg Masaru Yoshida Yoshihiro Nishimura 《Biochemical and biophysical research communications》2010,395(2):238-243
Asthma is a chronic inflammatory airway disease characterized by airway hyperreactivity, increased mucus production, and reversible airway contraction. Asthma is a complex genetic trait caused by environmental factors in genetically predisposed individuals. The transportation of maternal antigen-specific IgG via amniotic fluid, placenta and breast milk plays an important role in passive immunity. First, to examine whether maternal passive immunity by the transportation of antigen-specific IgG via FcRn regulates allergic airway inflammation, ovalbumin-immunized FcRn+/− female mice were bred with FcRn−/− male mice to evaluate the degree of ovalbumin-induced allergic airway inflammation of FcRn−/− offspring. Maternal passive immunity regulated allergic airway inflammation in an FcRn-dependent manner. Second, to examine the role of maternal antigen-specific IgG1 injection into mothers, we intravenously injected ovalbumin-specific IgG1 into wild-type or FcRn+/− mice immediately after they gave birth. The offspring were sensitized and challenged with ovalbumin. Antigen-specific IgG1 administered to lactating mice reduced allergic airway inflammation in their offspring in an FcRn-dependent manner. Last, to exclude the factor of maternal passive immunity other than ovalbumin-specific IgG1, we administered ovalbumin-specific IgG1 orally to offspring after birth. Oral administration of ovalbumin-specific IgG1 to offspring during the lactating period prevented the development of allergic airway inflammation in an FcRn-dependent manner. These data show that the transfer of maternal antigen-specific IgG regulates the development of allergic airway inflammation early in life in an FcRn-dependent manner. 相似文献
957.
958.
Misato Kobayashi Tamio Ohno Natsuko Hada Masato Fujiyoshi Masako Kuga Masahiko Nishimura Atsushi Murai Fumihiko Horio 《Journal of lipid research》2010,51(12):3463-3469
Each abdominal fat depot, such as mesenteric or epididymal, differently contributes to the development of insulin resistance. The aim of this study was to identify the genetic regions that contribute to fat accumulation in epididymal/mesenteric fat and to examine whether or not the genetic regions that affect glucose metabolism and body fat distribution are coincident. We previously mapped a major quantitative trait locus (QTL) (T2dm2sa) for impaired glucose tolerance on chromosome 2 and revealed that SM.A-T2dm2sa congenic mice showed not only glucose tolerance but also fat accumulation. In the present study, to identify the loci/genes that control the accumulation of abdominal fat, we perfomed QTL analyses of epididymal/mesenteric fat weight by using (A/J×SM.A-T2dm2sa)F2 mice in which the effect of T2dm2sa was excluded. As a result, two highly significant QTLs for mesenteric fat, as well as three significant QTLs for epididymal/mesenteric fat, were mapped on the different chromosomal regions. This suggests that the fat accumulations in individual fat depots are controlled by distinct genomic regions. Our comparison of these QTLs for abdominal fat distribution with those for glucose metabolism revealed that the major genetic factors affecting body fat distribution do not coincide with genetic factors affecting glucose metabolism in (A/J×SM.A-T2dm2sa)F2. 相似文献
959.
960.
Shirakawa M Ueda H Shimada T Koumoto Y Shimada TL Kondo M Takahashi T Okuyama Y Nishimura M Hara-Nishimura I 《The Plant journal : for cell and molecular biology》2010,64(6):924-935
SYP2 proteins are a sub-family of Qa-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) that may be responsible for protein trafficking between pre-vacuolar compartments (PVC) and vacuoles. Arabidopsis thaliana SYP22/VAM3/SGR3 and SYP21/PEP12 proteins function independently, but are both reported to be essential for male gametophytic viability. Here, we systematically examined the redundancy of three SYP2 paralogs (i.e. SYP21, 22 and 23) using a Col-0 ecotype harboring a SYP2 paralog (SYP23/PLP) that lacked a transmembrane domain. Surprisingly, no visible phenotypes were observed, even in the double knockout syp21/pep12 syp23/plp. Deficiency of either SYP21/PEP12 or SYP23/PLP in the syp22 background resulted in a defect in vacuolar protein sorting, characterized by abnormal accumulation of protein precursors in seeds. SYP21/PEP12 knockdown enhanced the syp22 phenotype (i.e. semi-dwarfism, poor leaf vein development and abnormal development of myrosin cells), and additional knockout of SYP23/PLP further aggravated the phenotype. A GFP-SYP23/PLP fusion localized to the cytosol, but not to the PVC or vacuolar membrane, where SYP21/PEP12 or SYP22/VAM3, respectively, were localized. Immunoprecipitation analysis showed that SYP23/PLP interacted with the vacuolar Qb- and Qc-SNAREs, VTI11 and SYP5, respectively, suggesting that SYP23/PLP is able to form a SNARE complex anchoring the membrane. Unexpectedly, we found that expression of multiple copies of a genomic fragment of SYP23/PLP suppressed the abnormal syp22-3 phenotype. Thus, SYP2 proteins, including cytosolic SYP23/PLP, appear to function redundantly in vacuolar trafficking and plant development. 相似文献