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21.
Kevbrin VV Zengler K Lysenko AM Wiegel J 《Extremophiles : life under extreme conditions》2005,9(5):391-398
A facultative aerobic, moderately thermophilic, spore forming bacterium, strain JW/VK-KG4 was isolated from an enrichment culture obtained from the Geyser valley, a geothermally heated environment located in the Kamchatka peninsula (Far East region of Russia). The cells were rod shaped, motile, peritrichous flagellated stained Gram positive and had a Gram positive type cell wall. Aerobically, the strain utilized a range of carbohydrates including glucose, fructose, trehalose, proteinuous substrates, and pectin as well. Anaerobically, only carbohydrates are utilized. When growing on carbohydrates, the strain required yeast extract and vitamin B12. Anaerobically, glucose was fermented to lactate as main product and acetate, formate, ethanol as minor products. Aerobically, even in well-aerated cultures (agitated at 500 rpm), glucose oxidation was incomplete and lactate and acetate were found in culture supernatants as by-products. Optimal growth of the isolate was observed at pH25 C 6.8–8.5 and 60°C. The doubling times on glucose at optimal growth conditions were 34 min (aerobically) and 40 min (anaerobically). The G+C content was 42.3 mol% as determined by Tm assay. Sequence analysis of the 16S rRNA gene indicated an affiliation of strain JW/VK-KG4 with Anoxybacillus species. Based on its morphology, physiology, phylogenetic relationship and its low DNA-DNA homology with validly published species of Anoxybacillus, it is proposed that strain JW/VK-KG4 represents a new species in the genus Anoxybacillus as A. kamchatkensis sp. nov. The type strain for the novel species is JW/VK-KG4T (=DSM 14988, =ATCC BAA-549). The GenBank accession number for the 16S rDNA sequence is AF510985. 相似文献
22.
Lytic action of an anaerobic proteolytic bacterium Proteinivorax tanatarense on organisms with different cell wall types was studied. In the absence of photosynthetic oxygen release, this proteolytic was able to grow on intact biomass of cyanobacteria belonging to various systematic groups. It is probably their usual saprotrophic satellite responsible for the regulation of abundance of primary producers during the dark phase. Growth also occurred on the biomass of a nonphototrophic gram-negative microorganism Halomonas campisalis, a common component of alkaliphilic microbial communities. Comparative analysis of the interaction of the proteolytic with H. campisalis cells at different physiological states revealed the lytic action to be restricted to dead and/or weakened cells, rather than to the actively dividing ones. Strict specificity of the action of the proteolytic bacterium on gram-negative microorganisms with no effect on gram-positive ones was shown. 相似文献
23.
Cellobiose metabolism was studied in Alkaliflexus imshenetskii, a haloalkaliphilic hydrolytic bacterium capable of utilizing certain polymers of plant origin, as well as mono- and disaccharides. The major products of cellobiose fermentation by the bacterium were succinate and acetate, and formate was a minor product. Cellobiose could be split into glucose molecules by both β-glucosidase (hydrolytic pathway) and phosphorylase (phosphorolytic pathway); the activity of the former enzyme was two orders of magnitude higher (3600 nmol/(min mg) versus 36 nmol/(min mg)). In cell extracts of the bacterium, high activities of the Embden-Meyerhof-Parnas pathway enzymes—hexokinase, glucose-phosphate isomerase, and phosphofructokinase—were revealed, as well as the activities of glucose-6-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, and key enzymes of the Entner-Doudoroff pathway—6-phospho-gluconate dehydratase and 2-keto-3-deoxy-6-phospho-gluconate aldolase. Neither the activity of the key enzyme of the hexose-mono-phosphate pathway, 6-phospho-gluconate dehydrogenase, nor the activities of the key enzymes of the modified Entner-Doudoroff pathway, glucose dehydrogenase and 2-keto-3-deoxy-gluconate kinase, were revealed. 相似文献
24.
Frolova A. A. Merkel A. Yu. Kevbrin V. V. Kopitsyn D. S. Slobodkin A. I. 《Microbiology》2023,92(1):21-29
Microbiology - An alkaliphilic, facultatively anaerobic bacterium (strain T05bT) was isolated from a terrestrial mud volcano on the Taman Peninsula, Russia. The cells of the isolate were motile... 相似文献
25.
Vadim V. Kevbrin Tatjana N. Zhilina Fred A. Rainey George A. Zavarzin 《Current microbiology》1998,37(2):94-100
Strain Z-7934, an alkaliphilic, obligately anaerobic, fermentative, asporogenous bacterium with Gram-positive cell wall structure,
was isolated from soda deposits in Lake Magadi, Kenya. The organism ferments only a few amino acids, preferentially arginine
and ornithine, with production of acetate, propionate, and ammonia. It is a true alkaliphile, with pH range for growth ranging
from 7.5 to 10.5 (optimum pH 8.5), and growth is dependent on the presence of sodium ions. The G+C content of the genomic
DNA is 37.6 mol%. 16S rDNA sequence analysis of strain Z-7934 shows that it belongs phylogenetically to cluster XI of the
low G+C Gram-positive bacteria. On the basis of its distinct phylogenetic position and unique physiological properties, we
propose a new genus and new species, Tindallia magadii, for this strain. The type strain is Z-7934T (=DSM 10318).
Received: 5 January 1998 / Accepted: 5 February 1998 相似文献
26.
Microbiology - The haloalkaliphilic microorganism Halonatronomonas betaini Z-7014Т was found to use glycine betaine as an electron acceptor in the Stickland reaction, resulting in its... 相似文献