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21.
Yasuaki Kagotani Setsuji Hisano Yoshihiro Tsuruo Shigeo Daikoku Yasuhiko Okimura Kazuo Chihara 《Cell and tissue research》1990,262(1):47-52
Summary Certain populations of arginine vasopressin (AVP) neurons in the magnocellular paraventricular nucleus became immunoreactive for neuropeptide Y (NPY) when rats were treated with colchicine or monosodium glutamate (MSG). The co-storage of these peptides was examined by empooying a post-embedding electron-microscopic immunohistochemistry technique using goldlabeled antibodies to the two peptides. In colchicinetreated rats, the neuronal perikarya contained numerous secretory granules showing co-storage of the two peptides. The cells of the MSG-treated rats were characterized by having well-developed Golgi bodies with the granular structures also co-storing the two peptides, although the secretory granules in the perikarya were rather fewer than in the colchicine-treated rats. It is concluded that the destruction of the arcuate nucleus by MSG-treatment may potentiate the synthesis of NPY in AVP neurons, the synthesis of which is latent in intact animals. 相似文献
22.
Increased frequency of 6-thioguanine-resistant peripheral blood lymphocytes in Werner syndrome patients 总被引:1,自引:1,他引:0
Ken-ichiro Fukuchi Kiyoji Tanaka Yuichi Kumahara Kazuo Marumo Matthew B. Pride George M. Martin Raymond J. Monnat Jr. 《Human genetics》1990,84(3):249-252
Summary The frequency of spontaneous 6-thioguanine (TG)-resistant peripheral blood lymphocytes in five unrelated Werner syndrome (WS) patients was determined using an autoradiographic labeling assay. The average frequency of TG-resistant lymphocytes was eightfold higher in WS patients than in sex- and age-matched normal control donors. This finding and previous identification of increased spontaneous chromosomal rearrangements and deletions in WS cells or cell lines suggest that WS is a human genomic instability or mutator syndrome. 相似文献
23.
Dr. Kazuo Tamura Masanori Takeuchi Nobuyuki Hirakawa Kiyokazu Toyoda Masaru Minoda 《Biotherapy》1990,2(3):223-226
The 35-year-old man with myelodysplastic syndrome (MDS) and granulocytopenia with dry cough and high fever was eventually found to have a left perinephric abscess ofStaphylococcus aureus. He underwent left nephrectomy and drainage of perinephric space in conjunction with appropriate antibiotics. However, because of persistent granulocytopenia,Staph. aureus never cleared up with formation of only poor granulation. Recombinant human granulocyte colony-stimulating factor (G-CSF) was added to the above treatment leading to prompt improvement in granulocytopenia and emergence of the good granulation tissue. G-CSF will probably become one of the important agents in treating MDS with granulocytopenia. 相似文献
24.
25.
Dr. Matthew D. Collins Reiner M. Kroppenstedt Jin Támaoka Kazuo Komagata Takeshi Kinoshita 《Current microbiology》1988,17(5):275-279
The structures of the tetrahydrogenated menaquinones fromActinomadura angiospora, Faenia rectivirgula, andSaccharothrix australiensis were determined by mass spectrometry and proton nuclear magnetic resonance spectrometry. The positions of saturation of the tetrahydrogenated menaquinones fromFaenia rectivirgula andSaccharothrix australiensis were units II plus III (counting from the ring system), whereas that ofActinomadura angiospora had units III and VIII hydrogenated. The tetrahydrogenated menaquinones fromFaenia rectivirgula andSaccharothrix australiensis are similar to those characterized from other Gram-positive taxa to date, whereas that fromActinomadura angiospora represents a hitherto unknown isomer. 相似文献
26.
Kageyuki Yamaoka Kazuo Ikeda 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1988,163(6):705-714
Summary Electrical excitability of the longitudinal ventrolateral body wall muscle of the third instar larva ofDrosophila melanogaster was demonstrated. This is in contrast to previous papers which have reported that this muscle is electrically inexcitable. It was found that an air supply to the muscle through the tracheoles is essential for maintaining its excitability. In an aerated preparation, the muscle maintained a resting potential of around –80 mV for more than 1.5 h, while a nonaerated muscle depolarized to about –30 mV within 30 min. Muscles with resting potentials larger than –70 mV showed graded regenerative potentials with a double-peaked configuration in response to transmembrane depolarizing current. A tetrodotoxin- (TTX-)sensitive, voltage-dependent inward sodium current, and a tetraethylammonium-(TEA-)sensitive, voltage-dependent outward potassium current were found to be responsible for the first peak of the electrogenic response of this muscle. The rising phase of the second peak was caused by a cobalt/manganese-sensitive, voltage-dependent inward calcium current that had a threshold level near –40 mV. Elimination by TEA or barium of the delayed rectification following the first peak caused the second peak to be triggered at a lower threshold. The second peak was profoundly elongated by barium, and this effect was antagonized by external calcium. Thus, the falling phase of the second peak was most likely driven by a calcium-dependent, outward potassium current. 相似文献
27.
Mitsuru Sakaizumi Yasuhiro Hashimoto Akemi Suzuki Tamio Yamakawa Yoshihiro Kiuchi Kazuo Moriwaki 《Immunogenetics》1988,27(1):57-60
The polymorphic variation of liver GM1 (NeuGc) ganglioside was found in inbred strains of the mouse. The genetic analysis using C57BL/10 (GM1-negative) and SWR (GM1-positive) mice revealed that a single autosomal gene (Ggm-1) was involved in the expression of liver GM1(NeuGc) and that C57BL/10 mice lacking GM1(NeuGc) expression carried a defective gene on Ggm-1. Since our previous study on H-2 congenic mice indicated that Ggm-1 was linked to the H-2 complex, in this study we measured recombination frequencies among Ggm-1, Go-1 and H-2K in the backcross progeny between (C57BL/10 × SWR)F1 and C57BL/10. Ggm-1 was mapped 1 cM centromeric to H-2K on chromosome 17.Abbreviations used in this paper GM1(NeuGc)
Gal1-3GalNAc1-4 (NeuGc2-3)Gal1-4Glc1-ceramide
- GM2(NeuGc)
Gal1-4(Neu Gc2-3)Gal1-4Glc1-ceramide
- GM3(NeuGc)
NeuGc2-3Gal1-4 Glc1-ceramide
- GD1a(NeuGc)
NeuGc2-3Gal1-3GalNAc1-4 (NeuGc2-3)Gal1-4Glc1-ceramide 相似文献
28.
Yasuhiko Asada Yoshio Kimura Masaaki Kuwahara Akira Tsukamoto Kazuo Koide Atsuhiro Oka Mitsuru Takanami 《Applied microbiology and biotechnology》1988,29(5):469-473
Summary A ligninase gene has been cloned from a Phanerochaete chrysosporium genomic DNA library. Nucleotide sequencing of the gene has revealed that the ligninase structural gene contains 1116 bp of the protein-encoding sequence, of which 84 bp encode the signal peptide. The protein-encoding sequence is interrupted by eight introns which conform to the universal G-T/A-G splicing rule observed for the 3 and 5 intron boundaries. The putative eukaryotic regulatory sequences, i.e. CAAT and TATA box-like sequences, are present in the 5 flanking region. 相似文献
29.
Levels of IAA, Cytokinins, ABA and Ethylene in Rice Plants as Affected by a Gibberellin Biosynthesis Inhibitor, Uniconazole-P. 总被引:1,自引:0,他引:1
Izumi Kazuo; Nakagawa Sachiko; Kobayashi Masatomo; Oshio Hiromichi; Sakurai Akira; Takahashi Nobutaka 《Plant & cell physiology》1988,29(1):97-104
Effects of uniconazole-P, a triazole-type growth retardant,on endogenous levels of IAA, cytokinins, ABA and ethylene inrice seedlings were investigated. Endogenous levels of IAA andABA were similar between control and uniconazole-P-treated riceshoots. Evolution of ethylene was promoted slightly, being 1.8times greater under 0.3 ppm uniconazole-P treatment than thatof control. The most obvious effect was the increase of trans-Zand trans-RZ in shoots. Shoots treated with uniconazole-P (10mg/m2 nursery box) contained 3.4 times and 3 times more trans-Zand trans-RZ than control, respectively. No significant differencesof cytokinin levels were recognized in roots except for cis-RZ.The increase of ethylene and active forms of cytokinins, andthe decrease of gibberellin in the shoots may be the basis forphysiological phenomena caused by uniconazole-P, namely thepromotion of flowering in woody plants and the enhancement offemaleness in cucumber. (Received September 9, 1987; Accepted October 20, 1987) 相似文献
30.
Kato Yoji; Otsuki Tatsuya; Nakajima Tasuku; Ojima Kunihiko; Matsuda Kazuo 《Plant & cell physiology》1988,29(4):539-547
-Glucans (average mol wt, 1.3 ? 104) extracted with perchloricacid from 8-day-old suspension-cultured nonglutinous (var. Sasanishiki)and glutinous rice (var. Miyakogane) cells were compared. Theresults of hydrolysis by alpha;-, ß- and iso-amylasesand methylation analysis of the -glucans suggested that theirbasic structures are almost the same. These -glucans are highly-branchedpolysaccharides with an average chain length of about 910,with exterior and interior chain lengths of about 67and 23, respectively.
1Current address: Laboratory of Food Science, Faculty of Education,Hirosaki University, Hirosaki, Aomori 036, Japan. (Received April 27, 1987; Accepted March 2, 1988) 相似文献