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341.
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343.
Masaki Fujita Tetsuhiro Moriya S. Fujimoto Nobuyuki Hara Kazunobu Amako 《Archives of microbiology》1997,167(4):196-201
The surface array protein (SAP) of Campylobacter fetus strain TK is encoded by seven homologous sapA genes clustered on the chromosomal DNA. The spontaneously arising variant strain TK(SAP–) produces no SAP and carries an approximately 10-kb chromosomal deletion. To elucidate the mechanism underlying the loss
of SAP synthesis, we analyzed the region containing the sapA homologues and the deletion. We constructed a physical map of the sapA cluster region by aligning the clones that contain sapA homologues. These analyses demonstrated that all sapA homologues were located within a limited region of about 50 kb of chromosomal DNA of strain TK. The TK(SAP–) deletion was located within this cluster and was 13.3 kb in size. The deletion occurred between two sapA homologues and resulted in the formation of a chimeric sapA homologue in the variant strain. Sequence analysis of the upstream regions and the conserved regions of all sapA homologues revealed a high degree of similarity. However, only one sapA homologue contained a putative promoter sequence. This promoter sequence was located in the deleted region. Thus, the deletion
of the promoter appears to be responsible for the loss of SAP expression in TK(SAP–).
Received: 17 May 1996 / Accepted: 6 December 1996 相似文献
344.
Sachiko Kaidzu Kazunobu Sugihara Masahiro Sasaki Aiko Nishiaki Tatsushi Igarashi Masaki Tanito 《Free radical research》2019,53(6):611-617
Two hundred twenty-two nanometres ultraviolet (UV) light produced by a krypton–chlorine excimer lamp is harmful to bacterial cells but not skin. However, the effects of 222-nm UV light exposure to the eye are not fully known. We evaluated acute corneal damage induced by 222- and 254-nm UV light in albino rats. Under deep anaesthesia, 6-week-old Sprague–Dawley albino rats were exposed to UV light. The exposure levels of corneal radiation were 30, 150, and 600?mJ/cm2. Epithelial defects were detected by staining with fluorescein. Superficial punctate keratitis developed in corneas exposed to more than 150?mJ/cm2 of UV light, and erosion was observed in corneas exposed to 600?mJ/cm2 of UV light. Haematoxylin and eosin staining also showed corneal epithelial defects in eyes exposed to 254-nm UV light. However, no damage developed in corneas exposed to 222-nm UV light. Cyclobutane pyrimidine dimer-positive cells were observed only in normal corneas and those exposed to 254-nm UV light. Although some epithelial cells were stained weakly in normal corneas, squamous epithelial cells were stained moderately, and the epithelial layer that was detached from the cornea exposed to 600?mJ/cm2 of light was stained intensely in corneas exposed to 254-nm UV light. In the current study, no corneal damage was induced by 222-nm UV light, which suggested that 222-nm UV light may not harm rat eyes within the energy range and may be useful for sterilising or preventing infection in the future. 相似文献
345.
Takahisa Kouwaki Toru Okamoto Ayano Ito Yukari Sugiyama Kazuo Yamashita Tatsuya Suzuki Shinji Kusakabe Junki Hirano Takasuke Fukuhara Atsuya Yamashita Kazunobu Saito Daisuke Okuzaki Koichi Watashi Masaya Sugiyama Sachiyo Yoshio Daron M. Standley Tatsuya Kanto Masashi Mizokami Kohji Moriishi Yoshiharu Matsuura 《Journal of virology》2016,90(7):3530-3542
346.
Morisaka Tadamichi Furuichi Tomo Kogi Kazunobu Yoshioka Motoi 《Journal of Ethology》2023,41(2):141-151
Journal of Ethology - The group definition for an animal population is important for building associations among individuals (i.e., social network). Indo-Pacific bottlenose dolphins (Tursiops... 相似文献
347.
Kazunobu Amako Norio Baba Nobuyuki Suzuki Sun Nyunt Wai Akiko Umeda 《Microbiology and immunology》1997,41(11):855-859
A negatively stained electron micrograph of regularly arranged porin proteins of Campylobacter jejuni on the isolated outer membrane of bacteria was analyzed in detail by the correlation averaging method using a computer-assisted program. The results showed that the porin of C. jejuni had a trimeric structure separated by about 10.4±0.15 nm. In addition, the pores in the trimers were also separated by about 4.3 ±0.1 nm. 相似文献
348.
Fine surface structure of enterotoxemic Escherichia coli O139:K12 strains associated with swine edema disease 总被引:1,自引:0,他引:1
The fine structure of the cell surface of seven enterotoxemic Escherichia coli (ETEEC) O139:K12 strains isolated from piglets with edema disease were examined electron microscopically using both the negative-staining
method and the freeze-substitution fixation method. Densely packed, fine fibers were observed; they consisted of a capsule
layer approximately 25 nm thick around the cell surfaces of strains 107/86, IW-2, ED-3, ED-43, and ED-61, all of which have
a capacity to adhere strongly to HEp-2 cells. In contrast, no such structure was observed on the surface of strains RK-O139
or ED-1, both of which adhere only weakly to HEp-2 cells. These results suggest that the capsule structure might be associated
with the ability to adhere to HEp-2 cells and, as a result, also potentially play some role in ETEEC infection.
Received: 29 April 1996 / Accepted: 13 August 1996 相似文献