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141.
142.
143.
Kanji Takeo Reiko Tanaka Makoto Miyaji Kazuko Nishimura 《FEMS microbiology letters》1995,129(2-3):231-235
Abstract Stationary-phase cells of Cryptococcus neoformans displayed two morphological characteristics: virtually all the cells were unbudded even in the early stationary phase and even when grown in rich media, and average cell size increased from that of exponential-phase cells. DNA contents for small and large stationary-phase cells were determined by quantitative fluorescence microscopy after DNA staining with propidium iodide or DAPI. Small cells contained G, DNA, whereas large unbudded cells had either a G2 or G1 DNA content, indicating that Cr. neoformans can enter into the stationary phase from either the G1 or G2 period. 相似文献
144.
Takuto Kojima Yasutomi Asano Osamu Kurasawa Yasuhiro Hirata Naoki Iwamura Tzu-Tshin Wong Bunnai Saito Yuta Tanaka Ryosuke Arai Kazuko Yonemori Yasufumi Miyamoto Yoji Sagiya Masahiro Yaguchi Sachio Shibata Akio Mizutani Osamu Sano Ryutaro Adachi Yoshinori Satomi Shinichi Imamura 《Bioorganic & medicinal chemistry》2018,26(9):2452-2465
We pursued serine palmitoyltransferase (SPT) inhibitors as novel cancer therapeutic agents based on a correlation between SPT inhibition and growth suppression of cancer cells. High-throughput screening and medicinal chemistry efforts led to the identification of structurally diverse SPT inhibitors 4 and 5. Both compounds potently inhibited SPT enzyme and decreased intracellular ceramide content. In addition, they suppressed cell growth of human lung adenocarcinoma HCC4006 and acute promyelocytic leukemia PL-21, and displayed good pharmacokinetic profiles. Reduction of 3-ketodihydrosphingosine, the direct downstream product of SPT, was confirmed under in vivo settings after oral administration of compounds 4 and 5. Their anti-tumor efficacy was observed in a PL-21 xenograft mouse model. These results suggested that SPT inhibitors might have potential to be effective cancer therapeutics. 相似文献
145.
146.
Kazuko Iwamoto Shuichi Kamo Yuichi Takada Ayana Ieda Takatoshi Yamashita Toshiro Sato Nobuhiro Zaima Tatsuya Moriyama 《Biochemistry and Biophysics Reports》2018
Soyasapogenol is a soyasaponin aglycone, which has been suggested to exert a more potent function than the glycoside form. In this study, the effect of soyasapogenol A and B on cultured adipocyte cell function was investigated using mouse 3T3-L1 adipocyte cells. 3T3-L1 cells were treated with insulin, dexamethasone, and 3-isobutyl-1-methylxanthine for differentiation to adipocytes, and the cells were then cultured in the presence of soyasapogenol A or B (6.25 or 12.5 µM). The media were harvested and refreshed every 2 d. After a 10 d culture, the cells were harvested and the triglyceride content of the cells was determined. The triglyceride content of soyasapogenol B-treated cells was significantly lower than those of vehicle-treated cells. Glycerol and free fatty acid levels in the soyasapogenol-treated cell media were higher than those in vehicle cells. However, there was no difference in the level of adipose triglyceride lipase among soyasapogenol A-, soyasapogenol B-, and vehicle-treated cells. The secreted adiponectin and resistin levels of soyasapogenol-treated cell media were also different compared with those of vehicle-treated cells. Especially, the secreted resistin level in soyasapogenol B-treated cell media was obviously reduced compared with that of vehicle-treated cells. Taken together, these results suggest that soyasapogenol B exerted an anti-obesity and anti-diabetic effect on adipocytes by lowering the cellular triglyceride level by accelerating triglyceride lipolysis with reduced resistin secretion. 相似文献
147.
Kazuko Handa Thayer White Sen-Itiroh Hakomori Setsuo Hirohashi 《Glycoconjugate journal》1997,14(1):39-43
Employing a new procedure, we established many monoclonal antibodies (mAbs) which inhibit E- or P-selectin-dependent cell adhesion. One of these mAbs is capable of staining selectin in paraffin-embedded histological sections. The procedure is based on immunization of BALB/c mice with irradiated mouse myeloma NS-1 cells (syngeneic HAT-sensitive fusion partner cells) transfected with cDNA encoding human E- or P-selectin. Resulting NS-1 transfectant cells permanently express human E- or P-selectin as immunogen. The mAbs are useful for detecting selectins by flow cytometric and immunohistological methods, and for inhibiting selectin-dependent adhesion in experimental models. In contrast, the majority of anti-selectin mAbs previously established do not have these capabilities. Abbreviations: Ig, immunoglobulin; mAb, monoclonal antibody 相似文献
148.
Ayako Sano Reiko Tanaka Kazuko Nishimura Cilmery S. Kurokawa Kunie labuki R. Coelho Marcello Franco Mario Rubens Montenegro Makoto Miyaji 《Mycoscience》1997,38(2):117-122
We have studied the physiological and morphological features of 17 isolates ofParacoccidioides brasiliensis in order to define their phenotypes. The isolates were cultured at room temperature on potato dextrose agar (PDA, Difco)
slants for mycelial growth and in 1% dextrose brain heart infusion agar (BHIA, Difco) at 37°C for the study of yeast forms.
Most mycelial and yeast forms grew well between pH 5.6–9.4. In their response to osmotic pressure the isolates were separated
in three groups: intolerant, intermediate and tolerant. They also varied in carbohydrate assimilation tests, which indicated
important metabolic variation. No clear differences were observed in phenol oxidase tests, KNO3, starch, casein and arbutin assimilation tests. Only 1 of the isolates, Bt-19, had gelatinase activity. No correlation was
observed between the above differences and virulence. Two patterns of growth were observed in the mycelial cultures, glabrous
and cottonous, the latter being correlated with increased virulence for ddY mice. Most yeast forms grew as cerebriform colonies,
but Pb-HC and Bt-19 colonies had a cobblestone-like surface. 相似文献
149.
Mikami K; Takahashi S; Katagiri T; Yamaguchi-Shinozaki K; Shinozaki K 《Journal of experimental botany》1999,50(334):729-730
Screening of an Arabidopsis cDNA library allowed the
isolation of a cDNA encoding a pleckstrin homology (PH) domain protein,
AtPH1, which consists of one PH domain with a short N-terminal extension.
According to its structural features, AtPH1 is proposed to be a plant
homologue of human pleckstrin. Northern blot analysis indicated that the
AtPH1 gene was expressed constitutively in all tissues
examined, with variation in the levels. The presence of a plant pleckstrin
homologue offers new insights into the biological function of the PH domain
in plant signalling. 相似文献
150.
Yasuo Yamazaki Hisashi Koike Yusuke Sugiyama Kazuko Motoyoshi Taeko Wada Shigeru Hishinuma Mitsuo Mita Takashi Morita 《European journal of biochemistry》2002,269(11):2708-2715
In this study, we isolated a 25-kDa novel snake venom protein, designated ablomin, from the venom of the Japanese Mamushi snake (Agkistrodon blomhoffi). The amino-acid sequence of this protein was determined by peptide sequencing and cDNA cloning. The deduced sequence showed high similarity to helothermine from the Mexican beaded lizard (Heloderma horridum horridum), which blocks voltage-gated calcium and potassium channels, and ryanodine receptors. Ablomin blocked contraction of rat tail arterial smooth muscle elicited by high K+-induced depolarization in the 0.1-1 microm range, but did not block caffeine-stimulated contraction. Furthermore, we isolated three other proteins from snake venoms that are homologous to ablomin and cloned the corresponding cDNAs. Two of these homologous proteins, triflin and latisemin, also inhibited high K+-induced contraction of the artery. These results indicate that several snake venoms contain novel proteins with neurotoxin-like activity. 相似文献